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Acute exercise boosts cell proliferation and the heat shock response in lymphocytes: correlation with cytokine production and extracellular-to-intracellular HSP70 ratio.
Heck, Thiago Gomes; Scomazzon, Sofia Pizzato; Nunes, Patrícia Renck; Schöler, Cinthia Maria; da Silva, Gustavo Stumpf; Bittencourt, Aline; Faccioni-Heuser, Maria Cristina; Krause, Mauricio; Bazotte, Roberto Barbosa; Curi, Rui; Homem de Bittencourt, Paulo Ivo.
Afiliação
  • Heck TG; Physiology Research Group, Department of Life Sciences, Postgraduate Program in Integral Attention to Health, Regional University of the Northwestern Rio Grande do Sul State, Rua do Comércio, 3000, Ijuí, RS, 98700-000, Brazil. gomesheck@yahoo.com.
  • Scomazzon SP; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil. gomesheck@yahoo.com.
  • Nunes PR; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil.
  • Schöler CM; Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy.
  • da Silva GS; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil.
  • Bittencourt A; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil.
  • Faccioni-Heuser MC; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil.
  • Krause M; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil.
  • Bazotte RB; Department of Morphological Sciences, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Curi R; Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 2nd floor, suite 350 lab 02, Porto Alegre, RS, 90050-170, Brazil.
  • Homem de Bittencourt PI; Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
Cell Stress Chaperones ; 22(2): 271-291, 2017 03.
Article em En | MEDLINE | ID: mdl-28251488
Exercise stimulates immune responses, but the appropriate "doses" for such achievements are unsettled. Conversely, in metabolic tissues, exercise improves the heat shock (HS) response, a universal cytoprotective response to proteostasis challenges that are centred on the expression of the 70-kDa family of intracellular heat shock proteins (iHSP70), which are anti-inflammatory. Concurrently, exercise triggers the export of HSP70 towards the extracellular milieu (eHSP70), where they work as pro-inflammatory cytokines. As the HS response is severely compromised in chronic degenerative diseases of inflammatory nature, we wondered whether acute exercise bouts of different intensities could alter the HS response of lymphocytes from secondary lymphoid organs and whether this would be related to immunoinflammatory responses. Adult male Wistar rats swam for 20 min at low, moderate, high or strenuous intensities as per an overload in tail base. Controls remained at rest under the same conditions. Afterwards, mesenteric lymph node lymphocytes were assessed for the potency of the HS response (42 °C for 2 h), NF-κB binding activity, mitogen-stimulated proliferation and cytokine production. Exercise stimulated cell proliferation in an "inverted-U" fashion peaking at moderate load, which was paralleled by suppression of NF-κB activation and nuclear location, and followed by enhanced HS response in relation to non-exercised animals. Comparative levels of eHSP70 to iHSP70 (H-index) matched IL-2/IL-10 ratios. We conclude that exercise, in a workload-dependent way, stimulates immunoinflammatory performance of lymphocytes of tissues far from the circulation and this is associated with H-index of stress response, which is useful to assess training status and immunosurveillance balance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-2 / Interleucina-10 / Proteínas de Choque Térmico HSP70 / Resposta ao Choque Térmico Limite: Animals Idioma: En Revista: Cell Stress Chaperones Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-2 / Interleucina-10 / Proteínas de Choque Térmico HSP70 / Resposta ao Choque Térmico Limite: Animals Idioma: En Revista: Cell Stress Chaperones Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda