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1.
Sci Rep ; 10(1): 6237, 2020 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-32277077

RESUMO

Tuberculosis (TB) is a significant public health problem in Ecuador with an incidence of 43 per 100,000 inhabitants and an estimated multidrug-resistant-TB prevalence in all TB cases of 9%. Genotyping of Mycobacterium tuberculosis (MTBC) is important to understand regional transmission dynamics. This study aims to describe the main MTBC lineages and sublineages circulating in the country. A representative sample of 373 MTBC strains from 22 provinces of Ecuador, with data comprising geographic origin and drug susceptibility, were genotyped using 24 loci-MIRU-VNTR. For strains with an ambiguous sublineage designation, the lineage was confirmed by Regions of Difference analysis or by Whole Genome Sequencing. We show that lineage 4 is predominant in Ecuador (98.3% of the strains). Only 4 strains belong to lineages 2-sublineage Beijing and two strains to lineage 3-sublineage Delhi. Lineage 4 strains included sublineages LAM (45.7%), Haarlem (31.8%), S (13.1%), X (4.6%), Ghana (0.6%) and NEW (0.3%). The LAM sublineage showed the strongest association with antibiotic resistance. The X and S sublineages were found predominantly in the Coastal and the Andean regions respectively and the reason for the high prevalence of these strains in Ecuador should be addressed in future studies. Our database constitutes a tool for MIRU-VNTR pattern comparison of M. tuberculosis isolates for national and international epidemiologic studies and phylogenetic purposes.


Assuntos
Antituberculosos/farmacologia , Resistência Microbiana a Medicamentos/genética , Mycobacterium tuberculosis/genética , Tuberculose/epidemiologia , Antituberculosos/uso terapêutico , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Equador/epidemiologia , Variação Genética , Humanos , Repetições Minissatélites/genética , Epidemiologia Molecular , Tipagem de Sequências Multilocus , Mycobacterium tuberculosis/efeitos dos fármacos , Mycobacterium tuberculosis/isolamento & purificação , Filogenia , Tuberculose/tratamento farmacológico , Tuberculose/microbiologia , Sequenciamento Completo do Genoma
2.
Int J Mycobacteriol ; 8(4): 366-370, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31793507

RESUMO

Background: Strains of the Beijing sublineage of Mycobacterium tuberculosis have caused large outbreaks of tuberculosis, often involving multidrug resistance strains and this genetically highly conserved family of strains predominates in some geographic areas. For most of the countries of Latin America, no country-wide studies about the prevalence of the Beijing lineage are available. Methods: In this study, we determine the prevalence of the Beijing sublineage in Ecuador, using a large nation-wide sample of 991 isolates from the years 2014-2016 and with the strains, in case-related-proportional representation, emerging from most of the provinces of the country. The isolates were genotyped with asinglenucleotidespecific polymorphism (SNP) polymerase chain reaction for the Beijing sublineage. SNPpositive strains were confirmed as belonging to this lineage with 24 mycobacterial interspersed repetitive unitvariable number of tandem repeat and DNA sequencing. Results: We identified only four Beijing isolates in this collection of 991 strains and calculated a prevalence rate of 0.43%. Conclusions: Our study shows a limited dissemination of the Beijing strains in the Ecuadorian population. This in contrast with the neighbor countries of Peru and Colombia were locally a prevalence of up to 16% has been reported.


Assuntos
Programas de Rastreamento/estatística & dados numéricos , Mycobacterium tuberculosis/genética , Polimorfismo de Nucleotídeo Único , Tuberculose/epidemiologia , Tuberculose/microbiologia , DNA Bacteriano/isolamento & purificação , Farmacorresistência Bacteriana , Equador/epidemiologia , Genótipo , Humanos , Repetições Minissatélites , Mycobacterium tuberculosis/classificação , Mycobacterium tuberculosis/efeitos dos fármacos , Reação em Cadeia da Polimerase , Prevalência , Análise de Sequência de DNA
3.
Microb Drug Resist ; 25(6): 931-937, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30883259

RESUMO

The Beijing family, the most successful Mycobacterium tuberculosis lineage, is considered hypervirulent, associated with clustering and has a strong association with multidrug-resistant tuberculosis. The Beijing strains have spread worldwide and also to Latin America. Genotyping of a countrywide collection of 380 M. tuberculosis strains from Ecuador, with 24-loci mycobacterial interspersed repetitive units-variable number tandem repeats (MIRU-VNTR), revealed only six Beijing strains, but four of these were MDR-TB. There was no clustering as all six strains had very distinct MIRU-VNTR profiles that have not been reported in the rest of Latin America. Although active transmission for Beijing has been described for the neighboring countries Peru and Colombia, there is no evidence that Beijing strains in Ecuador are more frequently transmitted than other strains. Moreover, the low prevalence (1.6%) of the Beijing sublineage in Ecuador challenges the concept of hyperadaptability and transmissibility of the Beijing strains in our country.


Assuntos
Variação Genética/genética , Mycobacterium tuberculosis/genética , Tuberculose Resistente a Múltiplos Medicamentos/genética , Tuberculose Resistente a Múltiplos Medicamentos/microbiologia , Antibacterianos/uso terapêutico , Pequim , Colômbia , DNA Bacteriano/genética , Equador/epidemiologia , Genótipo , Humanos , Repetições Minissatélites/genética , Mycobacterium tuberculosis/efeitos dos fármacos , Peru , Prevalência , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
4.
Arch Virol ; 162(4): 1083-1087, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27900468

RESUMO

The complete genomic sequence of a variant of the recently reported maize-associated totivirus (MATV) from China was obtained from commercial maize in Ecuador. The genome of MATV-Ec (Ecuador) (4,998 bp) is considerably longer than that of MATV-Ch (China) (3,956 bp), the main difference due to a ≈ 1-kb-long capsid-protein-encoding fragment that is completely absent from the Chinese genome. Sequence alignments between MATV-Ec and MATV-Ch showed an overall identity of 82% at the nucleotide level, whereas at the amino acid level, the viruses exhibited 95% and 94% identity for the putative capsid protein and the RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis of the viral RdRp domain indicated that MATV-Ec and MATV-Ch share a common ancestor with other plant-associated totiviruses, with Panax notoginseng virus A as the closest relative. MATV-Ec was detected in 46% (n = 80) of maize plants tested in this study, but not in endophytic fungi isolated from plants positive for the virus.


Assuntos
Genoma Viral , Doenças das Plantas/virologia , Totivirus/genética , Totivirus/isolamento & purificação , Zea mays/virologia , Sequência de Aminoácidos , Sequência de Bases , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Alinhamento de Sequência , Totivirus/química , Totivirus/classificação , Proteínas Virais/química , Proteínas Virais/genética
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