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Country-wide rapid screening for the Mycobacterium tuberculosis Beijing sublineage in Ecuador using a single-nucleotide polymorphism-polymerase chain reaction method.
Mora-Pinargote, Carlos; Garzon-Chavez, Daniel; Franco-Sotomayor, Greta; Leon-Benitez, Margarita; Granda-Pardo, Juan Carlos; Trueba, Gabriel; de Waard, Jacobus Henri; Garcia-Bereguiain, Miguel Angel.
Afiliação
  • Mora-Pinargote C; Laboratorio Para Investigaciones Biomedicas, Facultad de Ciencias de la Vida, Escuela Superior Politecnica del Litoral, Guayaquil, Ecuador.
  • Garzon-Chavez D; Instituto de Microbiologia, Universidad San Francisco de Quito, Quito, Ecuador.
  • Franco-Sotomayor G; Instituto Nacional de Salud Publica e Investigacion "Leopoldo Izquieta Perez", Guayaquil, Ecuador; Facultad de Ciencias de la Salud, Universidad Catolica Santiago de Guayaquil, Guayaquil, Venezuela.
  • Leon-Benitez M; Instituto Nacional de Salud Publica e Investigacion "Leopoldo Izquieta Perez", Guayaquil, Ecuador.
  • Granda-Pardo JC; Instituto Nacional de Salud Publica e Investigacion "Leopoldo Izquieta Perez", Guayaquil, Ecuador.
  • Trueba G; Instituto de Microbiologia, Universidad San Francisco de Quito, Quito, Ecuador.
  • de Waard JH; One Health Research Group, Universidad de las Americas, Quito; Laboratorio de Tuberculosis, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela, Caracas, Venezuela.
  • Garcia-Bereguiain MA; Laboratorio Para Investigaciones Biomedicas, Facultad de Ciencias de la Vida, Escuela Superior Politecnica del Litoral, Guayaquil, Ecuador; One Health Research Group, Universidad de las Americas, Quito, Venezuela.
Int J Mycobacteriol ; 8(4): 366-370, 2019.
Article em En | MEDLINE | ID: mdl-31793507
Background: Strains of the Beijing sublineage of Mycobacterium tuberculosis have caused large outbreaks of tuberculosis, often involving multidrug resistance strains and this genetically highly conserved family of strains predominates in some geographic areas. For most of the countries of Latin America, no country-wide studies about the prevalence of the Beijing lineage are available. Methods: In this study, we determine the prevalence of the Beijing sublineage in Ecuador, using a large nation-wide sample of 991 isolates from the years 2014-2016 and with the strains, in case-related-proportional representation, emerging from most of the provinces of the country. The isolates were genotyped with asinglenucleotidespecific polymorphism (SNP) polymerase chain reaction for the Beijing sublineage. SNPpositive strains were confirmed as belonging to this lineage with 24 mycobacterial interspersed repetitive unitvariable number of tandem repeat and DNA sequencing. Results: We identified only four Beijing isolates in this collection of 991 strains and calculated a prevalence rate of 0.43%. Conclusions: Our study shows a limited dissemination of the Beijing strains in the Ecuadorian population. This in contrast with the neighbor countries of Peru and Colombia were locally a prevalence of up to 16% has been reported.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Programas de Rastreamento / Polimorfismo de Nucleotídeo Único / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Humans País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Int J Mycobacteriol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Equador País de publicação: Índia
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Programas de Rastreamento / Polimorfismo de Nucleotídeo Único / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Humans País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Int J Mycobacteriol Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Equador País de publicação: Índia