RESUMO
BACKGROUND: Trauma due to external causes represents one of the greatest challenges for public health services in different regions around the world. This study aimed to determine the prevalence of facial trauma, associated risk factors, and classification of body injuries in individuals who underwent forensic examination in a Brazilian center. MATERIAL AND METHODS: Data were collected at the Ceará State Forensic Medicine unit in a 12-year period. Sociodemographic data related to the etiological agent and lesions resulting from the bodily injury were recorded. RESULTS: Among 1,031 physical injury exams, physical aggression (p<0.001), male victims aged between 21 and 30 years (p<0.001), salaried workers (p<0.001), and soft tissue and dentoalveolar injuries were significant findings. Regarding aggression, domestic violence was prevalent (p<0.001), perpetrated by the victim's partner (p<0.001), using a blunt instrument during the aggression (p<0.001), and directly associated with soft tissue injury (p<0.001). In traffic accidents, the most common type was motorcycle accident (p<0.001), on weekdays (p=0.036), at nighttime (p=0.134), showing a significant association with bone fractures (p=0.001). CONCLUSIONS: Oral and maxillofacial injuries obtained from a Brazilian forensic science center were significantly associated with sociodemographic and etiological factors. Key words:Forensic dentistry, facial trauma, violence, public health.
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So far, there is no report regarding the micro-Raman vibrational fingerprint of the bonds between 10-methacryloyloxy-decyl dihydrogen phosphate (10-MDP) and zirconia ceramics. Thus, the aim of this study was to identify the Raman vibrational peaks related to the bonds of 10-MDP with zirconia, as well as the influence on microshear bond strength. Micro-Raman spectroscopy was employed to assess the vibrational peak of 10-MDP binding to zirconia. Microshear bond strength of the dual-cure resin cement to zirconia with the presence of 10-MDP in composition of experimental ceramic primer and self-adhesive resin cement was also surveyed. Statistical analysis was performed by one-way ANOVA and Tukey's test (p < 0.05). Peaks at 1545 cm-1 and 1562 cm-1 were found to refer to zirconia binding with 10-MDP. The presence of 10-MDP in both experimental ceramic primer and self-adhesive resin cement improved microshear bond strength to zirconia ceramic. It can be concluded that the nondestructive method of micro-Raman spectroscopy was able to characterize chemical bonds of 10-MDP with zirconia, which improves the bond strengths of resin cement.
Assuntos
Metacrilatos/química , Cimentos de Resina/química , Estresse Mecânico , Zircônio/química , Cerâmica/química , Cerâmica/uso terapêutico , Colagem Dentária , Análise do Estresse Dentário , Humanos , Metacrilatos/uso terapêutico , Cimentos de Resina/uso terapêutico , Resistência ao Cisalhamento , Análise Espectral Raman , Vibração , Zircônio/uso terapêuticoRESUMO
OBJECTIVE: To evaluate the anti-inflammatory effect of pretreatment for three days with a fatty acid mixture with high ω-9:ω-6 ratio and low ω-6:ω-3 ratio on rats submitted to dental extraction. MATERIAL AND METHODS: Thirty-two male Wistar rats (270-310g) were randomly distributed in four groups (n=8/group): the sham control group and the negative control group received saline; the high omega-6/low omega-9 group received isolipid fatty acid with high ω-6:ω-3 ratio and low ω-9:ω-6 ratio; the high omega-3/low omega-6 group received fatty acid with low ω-6:ω-3 ratio and high ω-9:ω-6 ratio. Saline and oils were administered by gavage for 4days before exodontia and 3days after surgery, followed by euthanasia. Masseter edema was evaluated clinically and tissue samples were submitted to osteoclast count (H&E), myeloperoxidase assay, and western blotting (tumor necrosis factor-alpha and interleukin-1beta). RESULTS: In the high omega-3/low omega-6 group, a significant decrease was observed in masseter edema (p<0.0001), myeloperoxidase (p<0.0001), osteoclasts (p=0.0001) and TNF-α expression (p<0.0001), but not in IL-1ß expression. CONCLUSION: The ingestion of fatty acid with high ω-9:ω-6 ratio and low ω-6:ω-3 ratio significantly reduced inflammatory response in rats submitted to dental extraction.
Assuntos
Anti-Inflamatórios/farmacologia , Ácidos Graxos Ômega-3/farmacologia , Ácidos Graxos Ômega-6/farmacologia , Ácidos Graxos/farmacologia , Extração Dentária/métodos , Animais , Combinação de Medicamentos , Edema/tratamento farmacológico , Interleucina-1beta/análise , Masculino , Osteoclastos/efeitos dos fármacos , Peroxidase/análise , Ratos , Ratos Wistar , Fatores de Tempo , Resultado do Tratamento , Fator de Necrose Tumoral alfa/análise , Cicatrização/efeitos dos fármacosRESUMO
O ameloblastoma convencional é uma neoplasia benigna originada do tecido epitelial odontogênico encontrado nos ossos maxilares. É caracterizado, clinicamente, como tumor de crescimento lento, na maioria dos casos, é assintomático ou pode causar parestesias, assim como pode ocasionar deslocamento, mobilidade e reabsorção dental. Histologicamente, a variante multicística possui diversos padrões, como: folicular, plexiforme e desmoplásico. Devido a essas variações histo -patológicas, existem diversos métodos de tratamento, desde a enucleação e curetagem até ressecções mais agressivas. Sendo que essas são bem mais indicadas, tendo em vista o potencial agressivo e infiltrativo da lesão. Objetivo: este trabalho visa relatar um caso clínico de ameloblastoma multicístico tratado por ressecção marginal mandibular. Relato de caso: paciente do gênero feminino, 40 anos de idade, com hipótese diagnóstica de ameloblastoma por meio de radiografia panorâmica de rotina. Por intermédio da biópsia incisional, foi diagnosticado ameloblastoma multicístico em região de corpo, ângulo e ramo mandibular esquerdo. O tratamento de escolha foi, assim como na maioria dos casos relatados na literatura, a ressecção cirúrgica. Considerações finais: essa opção de tratamento é mais justificada pelo alto índice de recidiva das lesões tratadas de forma mais conservadora. No presente trabalho, a paciente foi mantida em proservação por trinta meses, período em que não foi observado nenhum sinal de recidiva da lesão ou complicações pós-operatórias.
RESUMO
A fratura do osso frontal é uma lesão rara quando comparada aos demais tipos de fraturas faciais. As fraturas do seio frontal normalmente estão associadas a trauma de grande impacto, estando na maioria dos casos associada à acidentes de trânsito. Pequena parcela dessas fraturas se apresenta proveniente de acidentes desportivos. Estas fraturas podem afetar a parede anterior ou posterior, com ou sem o envolvimento do ducto naso-frontal. O osso frontal está localizado em uma área de estruturas anatômicas importantes do ponto de vista estético e funcional, deste modo, o tratamento dessas fraturas se faz de extrema importância. Seu tratamento varia de acordo com o padrão da fratura, objetivando o retorno da estética e função. Este relato descreve o tratamento cirúrgico de fratura de parede anterior de seio frontal decorrente de acidente desportivo (Takendown), que ao exame clínico inicial apresentava diplopia para supraversão e perda de projeção anteroposterior do osso frontal. O tratamento instituído foi a fixação interna estável com placa e parafuso para o reestabelecimento estético e funcional. Após o acompanhamento ambulatorial o paciente teve alta sem quaisquer prejuízos estético-funcionais.
The fracture of the frontal bone is a rare lesion compared to other types of facial fractures. The frontal sinus fractures are usually associated with high-impact trauma, with the majority of cases associated with traffic accidents. With a smaller portion of these fractures arising from sports accidents. These fractures can affect the anterior or posterior wall, with or without the involvement of the naso-frontal duct. The frontal bone is located in an area of significant anatomical structures of the aesthetic and functional point of view, therefore, the treatment of these fractures becomes of paramount importance. Its treatment varies according to the kind of fracture, aiming at the return of aesthetics and function. This report describes the surgical treatment of fracture on anterior wall of the frontal sinus due to sporting accidents (Takendown), the initial clinical examination revealed diplopia for supraversion and loss of anteroposterior projection of the frontal bone. The treatment was the stable internal fixation with plate and screws for aesthetic and functional reestablishment. After outpatient, treatment the patient was discharged without any aesthetic funcional loses.
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Os ácidos graxos insaturados ômega-3 e ômega-9 possuem ação anti-inflamatóriae antioxidante, enquanto o ômega-6 possuiação pró-inflamatória.O objetivo do presente estudo foi avaliar a ação antioxidante e anti-inflamatória do mix de óleos em concentrações nutracêuticas com alta razão ômega-9: ômega-6, favorecendo ação antioxidante, e baixa razão ômega-6:ômega-3, favorecendo ação anti-inflamatória no terceiro dia pós exodontia em ratos. Foram utilizados trinta e dois ratos Wistar jovens com 270-310g distribuídos aleatoriamente em quatrogrupos de 8 animais: Grupo Sham, Grupo Solução Salina, Grupo mix isolipídico e Mix Teste. Os animais receberam NaCl 0,9%, Grupo Sham e Grupo Solução Salina, ou mix isolipídico,(ácido alfa-linoléico -ALA) mistura contendo ômega-6:ômega-3 óleos (relação de 8:01) e ômega-9:ômega-6 (relação 0,4:1), Grupo Isolipídico,ou Mix Teste (ácido alfa-linolênico -ALA, ácido docosahexaenóico -DHA, ácido eicosapentaenóico -EPA) de ômega-6:ômega-3 (relação 1,4:1) e ômega-9:ômega -6 (3,4:1 relação), Grupo Teste, receberam por gavagem1,2g da solução correspondente a cada grupo por kg/diadurantequatro dias antese três dias após a exodontia do primeiro molar inferior esquerdo. Foi realizadaa avaliação do edema gerado pelo procedimento cirúrgico e coletada a mucosa perialveolar ao sítio da extração dental e o próprio alvéolo dental para análises laboratoriais: mieloperoxidase, contagem de osteoclastos, expressão do TNF-α e IL-1β, glutationa (GSH) e substâncias reativas ao ácido tiobarbitúrico (TBARS)...
Oilfatty acids omega-3 and omega-9 haveanti-inflammatory and antioxidant activities, while the omega-6 haspro-inflammatory action. The aim of this study was to evaluate the presence of antioxidant and anti-inflammatory effects with high nutraceutical ratio of omega-9: omega-6, antioxidant properties, and low ratio omega-6: omega-3,anti-inflammatory propertiesthree days after tooth extraction in rats. Thirty-two young Wistar rats with 270-310g were randomly divided into four groups of 8 animals: Group Sham,Saline Group, Isolipid Group, and Mix Test Group. Theanimals received 0.9% NaCl, Sham Group and Salina Group, or Isolipid Mix(alpha-linolenic acid -ALA) mixture containing omega-6: omega-3 oils (ratio 8:01) and omega-9: omega-6 (ratio 0.4:1), Isolipidgroup, or Mix Test (alpha-linolenic acid -ALA, docosahexaenoic acid -DHA, eicosapentaenoic acid -EPA) omega-6: omega-3 (ratio 1.4: 1) and omega-9: omega -6 (3.4:1 ratio), Mix Text Group by gavage1.2g solutions for kg/dayfor four days before and three days after the extraction of the left mandibular firstmolar. Evaluation of edema generated by the surgical procedure and collected the perialveolar the site of tooth extraction and dental alveolar mucosa itself for laboratory testing wereperformed: myeloperoxidase, osteoclastcount, concentrationsof TNF-αand IL-1β, glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)...
Assuntos
Humanos , Inflamação , Estresse Oxidativo , Ácidos Graxos , MetabolismoRESUMO
Tumor odontogênico adenomatoide (TOA) é uma lesão benigna incomum de origem odontogênica, caracterizado pelo crescimento lento, progressivo, indolor, de consistência firme, cujo tamanho, normalmente não atinge grandes proporções. Há predileção pela região anterior de maxila e o envolvimento de um dente impactado; geralmente o canino, é recorrente, caracterizando a variante folicular. O TOA apresenta três variantes: folicular, extrafolicular e periférica, que não se distinguem microscopicamente, porém são diferenciadas com achados clínicos ou imaginológicos. O presente trabalho descreve um caso de tumor odontogênico adenomatoide folicular, presente em maxila tratado por enucleação e curetagem. O paciente encontra-se em acompanhamento pós-cirúrgico de 30 meses, sem quaisquer sinais clínicos e radiográficos de recidivas da lesão... (AU)
Adenomatoid odontogenic tumor (AOT) is an uncommon benign lesion of odontogenic origin, characterized by slow growth, progressive painless, firm consistency, with size that normally does not reach large proportions. There is a predilection for the anterior maxilla and the involvement of an impacted tooth, usually the canine, is recurrent, featuring follicular variant. The TOA have three variants: follicular, extrafollicular and peripheral, which are not distinguishable microscopically, but clinically or imaging. This paperdescribes a case of follicular adenomatoid odontogenic tumor present in the maxilla, treated by enucleation and curettage with 30 months follow-up without clinical and radiographic signs of injury recurrence... (AU)
Assuntos
Humanos , Feminino , Adulto , Dente Impactado/complicações , Tumores Odontogênicos/cirurgia , Odontoma , Adenoma/cirurgia , Maxila/cirurgia , Dente CaninoRESUMO
As fraturas faciais na infância são geralmente decorrentes de traumas de alta energia, e seu tratamento está relacionado à fase de crescimento facial. Esses tipos de traumas são objeto de atenção especial no que tange o diagnóstico e o tratamento devido a condições próprias da idade. A fixação interna estável (FIE), utilizando-se miniplacas/parafusos de titânio, pode ser utilizada com inteiro sucesso para o tratamento cirúrgico de fraturas mandibulares em crianças. Recentemente, destaca-se a utilização de materiais absorvíveis, os quais apresentam algumas vantagens sobre dispositivos metálicos em pacientes pediátricos. O sistema de fixação absorvível apresenta resultados satisfatórios em pacientes em fase de crescimento, pois não impede o desenvolvimento fisiológico dos ossos que sofreram fratura, evita a migração do material de fixação durante o período de crescimento, elimina artefatos em exames de imagem, evita sensibilidade térmica nos locais de instalação do material e elimina um segundo ato operatório para a remoção do material. Este trabalho tem por objetivo relatar um caso de fratura mandibular em paciente pediátrico, 11 anos de idade, tratado com miniplacas e parafusos absorvíveis pela equipe de Cirurgia e Traumatologia Buco-Maxilo-Facial do Hospital Universitário João de Barros Barreto... (AU)
Facial fractures in children are usually resulting from high-energy trauma and its treatment is related to facial growth phase. These types of traumas are object of special attention regarding the diagnosis and treatment because very conditions of age. The stable internal fixation using miniplates/titanium screws can be used with full success for the surgical treatment of mandibular fractures in children. Recently, we high light the use of absorbable materials, which exhibit certain advantages over metallic devices in pediatric patients. The absorbable fixation system shows satisfactory results in patients in the growth phase, since not prevent the physiological development of bones fractured, prevents the migration of fixing material during the growth period, eliminates artifacts in imaging studies, avoids thrmal sensitivity the installation locations of the material eliminates one second surgery for removal of the material. This study aims to report a case of mandibular fracture in a pediatric patient, 11 years old, treated with miniplates and absorbable screws by the staff of Surgery and Traumatology Buco- Maxillo-Facial University Hospital João de Barros Barreto... (AU)
Assuntos
Humanos , Masculino , Criança , Placas Ósseas , Acidentes de Trânsito , Ossos Faciais/lesões , Fixação Interna de Fraturas , Fraturas MandibularesRESUMO
Introduction:The dentigerous cyst, also called a follicular cyst is an odontogenic cyst that develops in association with crown of an impacted tooth, predominantly in mandibular third molars of young patients. The Odontoma is a ectomesenquimal tumor of unknown origin that are more considered developmental malformations (harmatomas) than benign neoplasms. Occasionally, the dentigerous cyst is associated with odontoma. Objective:The article aims to report a case of surgical treatment of dentigerous cyst associated with compound odontoma and unerupted tooth in anterior region of the mandible. Case report: A male patient, 17 years of age, without systemic changes, was sent to the Center for Dental Specialties of Horizonte, Ceará, Brazil, specialty of Oral and Maxillofacial Surgery, for diagnosis and treatment of oral lesions visualized after periapical radiographic examination to determine failed eruption of element 42. Observing the periapical radiograph it was possible to visualize radiopaque lesion suggestive of a compound odontoma and a cystic capsule in association with the element 42. A panoramic radiograph was requested and it was planned a surgical removal of odontoma with curettage of bone cavity and removing the cystic capsule and element 42. There were no significant postoperative complications, the suture removal was performed 7 days after surgery where it was possible to observe a good healing, no swelling and no paresthesia. Conclusion: The treatment of choice is surgical excision of the lesions with the tooth associated to the cyst, it should be performed with proper planning, avoiding injury to vital structures and should not be delayed in order to avoid possible occlusion sequelae.
RESUMO
Objetivo: Avaliar se existe relação entre os diferentes procedimentos odontológicos e o comportamento infantil. Material e método: Em crianças com idade entre oito e dez anos, pacientes da Clínica de Odontopediatria do Curso de Odontologia da Universidade Federal do Ceará foram utilizados para aferição de dor e desconforto: o questionário CFSS-DS, a classificação de Frankl, a Escala Visual Analógica, a Escala SOM, a Escala de Ansiedade da Face, e aferição dos batimentos cardíacos da criança. A amostra foi estratificada de acordo os procedimentos clínicos, grupo 1: procedimentos invasivos e grupo 2: procedimentos não invasivos. Resultados: No questionário CFSS-DS a amostra ficou caracterizada como de pouco medo para os dois grupos. Foi encontrado maior desconforto para procedimentos invasivos na Escala Analógica Facial, Escala Analógica Visual e Escala SOM. Por outro lado não foi encontrado um padrão para os batimentos cardíacos dentro de cada grupo. Verificou-se que não há grande diferença entre procedimentos invasivos e procedimentos não invasivos do ponto de vista comportamental da criança. Pois, nos parâmetros utilizados, como: Batimentos Cardíacos, Escala Visual Analógica, Escala Analógica Facial Depois e Escala SOM, houve uma sensação de desconforto em ambos os procedimentos. Percebe-se então, a necessidade constante da utilização de técnicas de controle e condicionamento comportamental e psicológico para a realização do atendimento ao paciente pediátrico.
Objective: To evaluate whether there is a relationship between the different dental procedures and child behavior. Methods: In children aged eight and ten, patients of the Clinic of Pediatric Dentistry of the Federal University of Ceará Dentistry Course were used to measure pain and discomfort: the CFSS-DS questionnaire, the Frankl classification, the scale Visual Analog, Scale SOM, the Face Anxiety Scale, and measurement of the child's heartbeat. The sample was stratified by clinical procedures, group 1: invasive procedures and group 2: non-invasive procedures. Results: In the CFSS-DS questionnaire sample was characterized as little scared for both groups. It found greater discomfort for invasive procedures in Facial Analogue Scale, Visual Analogue Scale and Scale SOM. On the other hand it was not found a pattern to the heartbeat within each group. Conclusion: It was found that no great difference between invasive and non-invasive procedures behavioral point of view of the child. For the used parameters, such as: Heart Rate, Visual Analogue Scale, Analog Scale and Scale Facial After SOM, there was a sense of discomfort in both procedures. It can be seen then, the constant need to use control techniques and behavioral and psychological conditioning for the realization of care to pediatric patients.
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O Granuloma central de células gigantes (GCCG) é uma entidade não neoplásica, tendo em vista suas características clínicas e imaginológicas faz-se a definição do padrão da lesão em agressiva ou não-agressiva que irá determinar o tratamento de escolha, quando agressiva causa destruição óssea considerável. O presente trabalho visa relatar um caso clínico de Granuloma Central de Células Gigantes agressiva em maxila com tratamento cirúrgico de enucleação com acesso cirúrgico de Weber Ferguson, atualmente esse paciente encontra-se com dezoito meses de acompanhamento sem sinais de recidiva... (AU)
Central giant cell granuloma is a non-neoplastic entity in which the clinical and imaging characterisitics identify the injury as an aggressive or nonaggressive lesion, a distinction that will determine the choice of treatment, bearing in mind that the aggressive form may cause significant bone destruction. This paper describes a case of aggressive central giant cell granuloma in the maxilla treated surgically using the Weber Ferguson approach. After eighteen months of follow-up the patient shows no signs of recurrence... (AU)
Assuntos
Humanos , Masculino , Adolescente , Granuloma de Células Gigantes , Granuloma de Células Gigantes/cirurgia , Granuloma de Células Gigantes/diagnóstico por imagem , Doenças Maxilares , Arcada Osseodentária/fisiopatologia , Maxila/patologiaRESUMO
PURPOSE: To evaluate the antioxidant and antiperoxidative effects of oil mixes of high ratio Omega-9:Omega-6 and low ratio Omega-6:Omega-3 in the third day after tooth extraction in rats. METHODS: Thirty-two male Wistar rats (270-310 g) were randomly distributed in two groups: Control (n=24) and Test (n=8). Control group was divided into three subgroups (n=8): G1: Sham-Saline; G2: Saline; G3: Isolipid. G1 and G2 animals received NaCl 0.9% while G3 rats were treated with an isolipid mixture (alpha-linolenic acid - ALA) containing -6/-3 oils (8:1 ratio) and-9/-6 (0.4:1 ratio). Test group animals (G4) received oily mixtures (alpha-linolenic acid - ALA, docosahexaenoic acid - DHA, eicosapentaenoic acid - EPA) of -6/-3 (1.4:1 ratio) and -9/-6 (3.4:1 ratio). Saline and oils were administered by gavage during four days before and three days after first mandibular molar extraction. Following, samples (arterial blood and alveolar mucosa) were collected for glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) assays. RESULTS: Oil mixes induced a significant decrease in GSH and TBARS tissue and plasma concentrations in the third day post-surgery. CONCLUSION: Gavage administration of oil mixes of high ratio Omega-9:Omega-6 and low ratio Omega-6:Omega-3 after molar extraction in rats induces a significant decrease in lipid peroxidation.
Assuntos
Ácidos Graxos Ômega-3/farmacologia , Ácidos Graxos Ômega-6/farmacologia , Ácidos Graxos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extração Dentária/métodos , Animais , Combinação de Medicamentos , Glutationa/análise , Glutationa/efeitos dos fármacos , Masculino , Dente Molar/cirurgia , Distribuição Aleatória , Ratos Wistar , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Fatores de Tempo , Alvéolo Dental/efeitos dos fármacos , Resultado do Tratamento , Cicatrização/efeitos dos fármacosRESUMO
PURPOSE: To evaluate the antioxidant and antiperoxidative effects of oil mixes of high ratio Omega-9:Omega-6 and low ratio Omega-6:Omega-3 in the third day after tooth extraction in rats. METHODS: Thirty-two male Wistar rats (270-310g) were randomly distributed in two groups: Control (n=24) and Test (n=8). Control group was divided into three subgroups (n=8): G1: Sham-Saline; G2: Saline; G3: Isolipid. G1 and G2 animals received NaCl 0.9% while G3 rats were treated with an isolipid mixture (alpha-linolenic acid - ALA) containing -6/-3 oils (8:1 ratio) and-9/-6 (0.4:1 ratio). Test group animals (G4) received oily mixtures (alpha-linolenic acid - ALA, docosahexaenoic acid - DHA, eicosapentaenoic acid - EPA) of -6/-3 (1.4:1 ratio) and -9/-6 (3.4:1 ratio). Saline and oils were administered by gavage during four days before and three days after first mandibular molar extraction. Following, samples (arterial blood and alveolar mucosa) were collected for glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) assays. RESULTS: Oil mixes induced a significant decrease in GSH and TBARS tissue and plasma concentrations in the third day post-surgery. CONCLUSION: Gavage administration of oil mixes of high ratio Omega-9:Omega-6 and low ratio Omega-6:Omega-3 after molar extraction in rats induces a significant decrease in lipid peroxidation. .
Assuntos
Animais , Masculino , /farmacologia , /farmacologia , Ácidos Graxos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extração Dentária/métodos , Combinação de Medicamentos , Glutationa/análise , Glutationa/efeitos dos fármacos , Dente Molar/cirurgia , Distribuição Aleatória , Ratos Wistar , Fatores de Tempo , Resultado do Tratamento , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Alvéolo Dental/efeitos dos fármacos , Cicatrização/efeitos dos fármacosRESUMO
To evaluate the antioxidant and antiperoxidative effects of oil mixes of high ratio Omega-9:Omega-6 and low ratio Omega-6:Omega-3 in the third day after tooth extraction in rats. Thirty-two male Wistar rats (270-310g) were randomly distributed in two groups: Control (n=24) and Test (n=8). Control group was divided into three subgroups (n=8): G1: Sham-Saline; G2: Saline; G3: Isolipid. G1 and G2 animals received NaCl 0.9% while G3 rats were treated with an isolipid mixture (alpha-linolenic acid - ALA) containing -6/-3 oils (8:1 ratio) and-9/-6 (0.4:1 ratio). Test group animals (G4) received oily mixtures (alpha-linolenic acid - ALA, docosahexaenoic acid - DHA, eicosapentaenoic acid - EPA) of -6/-3 (1.4:1 ratio) and -9/-6 (3.4:1 ratio). Saline and oils were administered by gavage during four days before and three days after first mandibular molar extraction. Following, samples (arterial blood and alveolar mucosa) were collected for glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) assays. Oil mixes induced a significant decrease in GSH and TBARS tissue and plasma concentrations in the third day post-surgery. Gavage administration of oil mixes of high ratio Omega-9:Omega-6 and low ratio Omega-6:Omega-3 after molar extraction in rats induces a significant decrease in lipid peroxidation.(AU)
Assuntos
Animais , Ratos , Antioxidantes/análise , Ácidos Graxos Ômega-3/análise , Ácidos Graxos Ômega-6/análise , Extração Dentária/veterinária , Ratos/classificaçãoRESUMO
PURPOSE: To evaluate the effect of bioflavonoid ternatin (TRT) on rat liver regeneration and oxidative stress after 70% partial hepatectomy (PH). METHODS: Thirty six young male Wistar rats were randomly assigned to two groups of 18 animals each - control (G1) and experimental (G2) - and were submitted to PH under inhalatory diethylether anesthesia. G1 rats received daily intraperitoneal (ip) injections of saline (NaCl 0.9% solution) 0.1 mL/kg for 14 days; G2 animals received daily ip injections of TRT 0.1% 1.0mg/kg for 14 days. At 36h (T1), 168h (T2) and 336h (T3) post-PH timepoints, a subgroup of six rats in each group was chosen in a randomized way to complementary hepatectomy (CH) and blood samples haversting. Collected material was saved for laboratory analysis (total bilirubin (TB), D-Glucose, glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) and assessment of liver regeneration. RESULTS: TRT induced a significant decrease in liver and plasma GSH concentrations; liver regeneration process was not affected. TRT promoted a significant decrease in blood glucose levels 168h after partial hepatectomy compared with controls. TB levels remained unchanged. CONCLUSION: Intraperitoneal bioflavonoid ternatin injection in partially hepatectomized rats induces a decrease in oxidative stress and a significant hypoglycemic state, but does not promote any change in the evolution of liver regeneration.
Assuntos
Flavonoides/farmacologia , Regeneração Hepática/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Animais , Antioxidantes/farmacologia , Bilirrubina/sangue , Glucose/análise , Glutationa/sangue , Hepatectomia/métodos , Injeções Intraperitoneais , Fígado/metabolismo , Regeneração Hepática/fisiologia , Masculino , Tamanho do Órgão , Distribuição Aleatória , Ratos , Ratos Wistar , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Fatores de TempoRESUMO
PURPOSE: To evaluate the effect of bioflavonoid ternatin (TRT) on rat liver regeneration and oxidative stress after 70% partial hepatectomy (PH). METHODS: Thirty six young male Wistar rats were randomly assigned to two groups of 18 animals each - control (G1) and experimental (G2) - and were submitted to PH under inhalatory diethylether anesthesia. G1 rats received daily intraperitoneal (ip) injections of saline (NaCl 0.9% solution) 0.1 mL/kg for 14 days; G2 animals received daily ip injections of TRT 0.1% 1.0mg/kg for 14 days. At 36h (T1), 168h (T2) and 336h (T3) post-PH timepoints, a subgroup of six rats in each group was chosen in a randomized way to complementary hepatectomy (CH) and blood samples haversting. Collected material was saved for laboratory analysis (total bilirubin (TB), D-Glucose, glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) and assessment of liver regeneration. RESULTS: TRT induced a significant decrease in liver and plasma GSH concentrations; liver regeneration process was not affected. TRT promoted a significant decrease in blood glucose levels 168h after partial hepatectomy compared with controls. TB levels remained unchanged. CONCLUSION: Intraperitoneal bioflavonoid ternatin injection in partially hepatectomized rats induces a decrease in oxidative stress and a significant hypoglycemic state, but does not promote any change in the evolution of liver regeneration.
Assuntos
Animais , Masculino , Ratos , Flavonoides/farmacologia , Regeneração Hepática/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Antioxidantes/farmacologia , Bilirrubina/sangue , Glucose/análise , Glutationa/sangue , Hepatectomia/métodos , Injeções Intraperitoneais , Regeneração Hepática/fisiologia , Fígado/metabolismo , Tamanho do Órgão , Distribuição Aleatória , Ratos Wistar , Fatores de Tempo , Substâncias Reativas com Ácido Tiobarbitúrico/análiseRESUMO
PURPOSE: To evaluate the effect of bioflavonoid ternatin (TRT) on rat liver regeneration and oxidative stress after 70% partial hepatectomy (PH). METHODS: Thirty six young male Wistar rats were randomly assigned to two groups of 18 animals each - control (G1) and experimental (G2) - and were submitted to PH under inhalatory diethylether anesthesia. G1 rats received daily intraperitoneal (ip) injections of saline (NaCl 0.9% solution) 0.1 mL/kg for 14 days; G2 animals received daily ip injections of TRT 0.1% 1.0mg/kg for 14 days. At 36h (T1), 168h (T2) and 336h (T3) post-PH timepoints, a subgroup of six rats in each group was chosen in a randomized way to complementary hepatectomy (CH) and blood samples haversting. Collected material was saved for laboratory analysis (total bilirubin (TB), D-Glucose, glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) and assessment of liver regeneration. RESULTS: TRT induced a significant decrease in liver and plasma GSH concentrations; liver regeneration process was not affected. TRT promoted a significant decrease in blood glucose levels 168h after partial hepatectomy compared with controls. TB levels remained unchanged. CONCLUSION: Intraperitoneal bioflavonoid ternatin injection in partially hepatectomized rats induces a decrease in oxidative stress and a significant hypoglycemic state, but does not promote any change in the evolution of liver regeneration.(AU)
Assuntos
Animais , Ratos , Regeneração Hepática/fisiologia , Estresse Oxidativo , Hipoglicemia , Ratos/classificação , FlavonoidesRESUMO
OBJECTIVE: To study the effects of polyunsaturated fatty acids (PUFA) omega-3 and omega-6 in the oxidative stress and in liver regeneration in rats subjected to 70% partial hepatectomy (PH, 70% hepatectomy, Higgins-Anderson partial hepatectomy). METHODS: 72 young male Wistar rats were randomly divided into four equal-sized groups: control (G1), partially hepatectomized (G2), partially hepatectomized with two weeks daily intraperitoneal infusion of omega-3 (G3) and partially hepatectomized with two weeks daily intraperitoneal infusion of omega-6 (G4). In moments 36 h (T1), 168 h (T2) and 336 h (T3) post-PH, thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in plasma and liver tissue, while glucose and total bilirubin were measured in blood. The mass of the residual liver in the same moments was the parameter used to estimate the evolution of liver regeneration. RESULTS: omega-3 PUFA inhibited liver regeneration and induced reduction of hepatic GSH concentration seven days post-PH. Omega-6 PUFA, in contrast, showed no inhibitory effect on regeneration. There was an increase of lipid peroxidation both in blood and liver with administration of omega-6. CONCLUSION: Omega-3 PUFA retarded post-PH liver regeneration, probably through inhibition of oxidative stress. Omega-6 PUFA increased TBARS concentrations in blood and liver but did not alter the evolution of the liver regenerative process.
Assuntos
Ácidos Graxos Ômega-3/farmacologia , Ácidos Graxos Ômega-6/farmacologia , Regeneração Hepática/efeitos dos fármacos , Animais , Masculino , Ratos , Ratos WistarRESUMO
OBJETIVO: Estudar os efeitos dos ácidos graxos poli-insaturados (PUFA) ômega-3 e ômega-6 no estresse oxidativo e na regeneração hepática em ratos submetidos à hepatectomia parcial à 70 por cento (HP, hepatectomia a 70 por cento, hepatectomia parcial à Higgins-Anderson). MÉTODOS: 72 ratos Wistar machos jovens foram aleatoriamente distribuídos em quatro grupos de mesmo tamanho: controle, parcialmente hepatectomizados, e parcialmente hepatectomizados com aporte diário intraperitoneal, por duas semanas, de ou ômega-3 ou ômega-6. Nos tempos 36h (T1), 168h (T2) e 336h (T3) pós-HP, substâncias reativas ao ácido tiobarbitúrico (TBARS) e glutationa reduzida (GSH) foram medidos no plasma e no tecido hepático, enquanto glicose e bilirrubina total foram aquilatados no sangue. A massa do fígado residual, nos mesmos tempos, foi o parâmetro utilizado para estimar a evolução da regeneração hepática. RESULTADOS: PUFA ômega-3 inibiu a regeneração hepática e induziu redução na concentração de GSH hepático sete dias pós-HP. PUFA ômega-6, ao contrário, não mostrou efeito inibitório sobre a regeneração. Houve aumento da peroxidação lipídica tanto no sangue como no fígado com a administração de ômega-6. CONCLUSÃO: PUFA ômega-3 retardou a regeneração hepática pós-HP provavelmente por inibição do estresse oxidativo. PUFA ômega-6 aumentou as concentrações de TBARS no sangue e no fígado mas não alterou a evolução do processo regenerativo hepático.
OBJECTIVE: To study the effects of polyunsaturated fatty acids (PUFA) omega-3 and omega-6 in the oxidative stress and in liver regeneration in rats subjected to 70 percent partial hepatectomy (PH, 70 percent hepatectomy, Higgins- Anderson partial hepatectomy). METHODS: 72 young male Wistar rats were randomly divided into four equal-sized groups: control (G1), partially hepatectomized (G2), partially hepatectomized with two weeks daily intraperitoneal infusion of omega-3 (G3) and partially hepatectomized with two weeks daily intraperitoneal infuison of omega-6 (G4). In moments 36h (T1), 168h (T2) and 336h (T3) post-PH, thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in plasma and liver tissue, while glucose and total bilirubin were measured in blood. The mass of the residual liver in the same moments was the parameter used to estimate the evolution of liver regeneration. RESULTS: omega-3 PUFA inhibited liver regeneration and induced reduction of hepatic GSH concentration seven days post-PH. Omega-6 PUFA, in contrast, showed no inhibitory effect on regeneration. There was an increase of lipid peroxidation both in blood and liver with administration of omega-6. CONCLUSION: Omega-3 PUFA retarded post-PH liver regeneration, probably through inhibition of oxidative stress. Omega-6 PUFA increased TBARS concentrations in blood and liver but did not alter the evolution of the liver regenerative process.