RESUMO
Candida tropicalis was identified as the etiologic agent of a severe contamination detected on an industrial fodder yeast production at the Cuban eastern region. After a detailed diagnostic task on raw material carried out on different factory sections, protocols to identify the contamination source and to isolate the microorganism were proposed. The identification was by comparison of the internal transcribed spacers ITS1 and ITS4 from 5.8S ribosomal DNA nucleotide sequences. In parallel, propagation of production strain, Candida utilis NRRL Y-660, at lab scale (2.5 l) was performed. Similar results to those observed in the factory concerning to its kinetic behavior in aerobic propagation with contaminated molasses, were detected at this level. The identification and primary kinetic characterization led to the implementation of sanitary and technological measures to bring production at its normal operational conditions as well as the application of prophylactic surveillance methodologies to avoid future contaminations.
Assuntos
Ração Animal/microbiologia , Candida tropicalis/isolamento & purificação , Contaminação de Alimentos , Microbiologia de Alimentos , Microbiologia Industrial/métodos , Sequência de Bases , Candida/crescimento & desenvolvimento , Candida tropicalis/genética , Cuba , DNA Fúngico/análise , DNA Espaçador Ribossômico/análise , Fermentação , Cinética , Melaço/microbiologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , RNA Ribossômico 5,8S/análiseRESUMO
Se identificò a Candida tropicalis como el agente causal de una severa contaminaciòn detectada en la producciòn de levadura forrajera en una fàbrica de la regiòn oriental de Cuba. Posteriormente a un detallado diagnòstico en las materia primas y diferentes secciones de la fàbrica con el objetivo de identifcar el foco y aislar el microorganismo contaminante se procediò a su identificaciòn mediante comparaiòn de sustancias nucleòtidas de las regiones adyacentes no codificadas...(AU)
Candida tropicalis was identified as the etiologic agent of a severe contamination detected on an industrial fodder yeast production at the Cuban eastern region. After a detailed diagnostic task on raw material carried out on different factory sections, protocols to identify the contamination source and to isolate the microorganism were proposed...(AU)