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[Detection and kinetic characterization of Candida tropicalis contamination during fodder yeast production]. / Detección y caracterización cinética de una contaminación por Candida tropicalis durante la producción de levadura forrajera.
García Gutiérrez, Roxana; Otero Rambla, Miguel Angel; Fundora Toucet, Norka; Martínez Sánchez, Aidín; Saura Lauria, Gustavo; Casalot, Laurence.
Afiliação
  • García Gutiérrez R; Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar, Apartado postal 4026, La Habana, Cuba. roxana.garcia@icidca.edu.cu
Rev Iberoam Micol ; 23(2): 90-3, 2006 Jun.
Article em Es | MEDLINE | ID: mdl-16854184
Candida tropicalis was identified as the etiologic agent of a severe contamination detected on an industrial fodder yeast production at the Cuban eastern region. After a detailed diagnostic task on raw material carried out on different factory sections, protocols to identify the contamination source and to isolate the microorganism were proposed. The identification was by comparison of the internal transcribed spacers ITS1 and ITS4 from 5.8S ribosomal DNA nucleotide sequences. In parallel, propagation of production strain, Candida utilis NRRL Y-660, at lab scale (2.5 l) was performed. Similar results to those observed in the factory concerning to its kinetic behavior in aerobic propagation with contaminated molasses, were detected at this level. The identification and primary kinetic characterization led to the implementation of sanitary and technological measures to bring production at its normal operational conditions as well as the application of prophylactic surveillance methodologies to avoid future contaminations.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia Industrial / Contaminação de Alimentos / Candida tropicalis / Microbiologia de Alimentos / Ração Animal Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies País/Região como assunto: Caribe / Cuba Idioma: Es Revista: Rev Iberoam Micol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia Industrial / Contaminação de Alimentos / Candida tropicalis / Microbiologia de Alimentos / Ração Animal Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies País/Região como assunto: Caribe / Cuba Idioma: Es Revista: Rev Iberoam Micol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha