RESUMO
BACKGROUND: Bone marrow-derived stem cell transplantation is a potentially viable therapeutic option for the treatment of neurodegenerative disease. MATERIAL/METHODS: We have isolated bone marrow stem cells by standard method. We then evaluated the survival of rats' bone marrow mononuclear cells implanted in rats' brain. The cells were extracted from rats' femurs, and marked for monitoring purposes by adenoviral transduction with Green Fluorescent Protein (GFP). Labeled cells were implanted within the area of rats' striatum lesions that were induced a month earlier employing quinolinic acid-based method. The implants were phenotyped by monitoring CD34; CD38; CD45 and CD90 expression. Bone marrow stromal cells were extracted from rats' femurs and cultivated until monolayer bone marrow stromal cells were obtained. The ability of bone marrow stromal cells to express NGF and GDNF was evaluated by RT-PCR. RESULTS: Implanted cells survived for at least one month after transplantation and dispersed from the area of injection towards corpus callosum and brain cortex. Interestingly, passaged rat bone marrow stromal cells expressed NGF and GDNF mRNA. CONCLUSIONS: The bone marrow cells could be successfully transplanted to the brain either for the purpose of trans-differentiation, or for the expression of desired growth factors(AU)
Assuntos
Animais , Masculino , Células da Medula Óssea/citologia , Encéfalo/patologia , Transplante de Tecido Encefálico , Sobrevivência de Enxerto , Neurônios/metabolismo , Transplante de Células-TroncoRESUMO
BACKGROUND: Bone marrow-derived stem cell transplantation is a potentially viable therapeutic option for the treatment of neurodegenerative disease. MATERIAL/METHODS: We have isolated bone marrow stem cells by standard method. We then evaluated the survival of rats' bone marrow mononuclear cells implanted in rats' brain. The cells were extracted from rats' femurs, and marked for monitoring purposes by adenoviral transduction with Green Fluorescent Protein (GFP). Labeled cells were implanted within the area of rats' striatum lesions that were induced a month earlier employing quinolinic acid-based method. The implants were phenotyped by monitoring CD34; CD38; CD45 and CD90 expression. Bone marrow stromal cells were extracted from rats' femurs and cultivated until monolayer bone marrow stromal cells were obtained. The ability of bone marrow stromal cells to express NGF and GDNF was evaluated by RT-PCR. RESULTS: Implanted cells survived for at least one month after transplantation and dispersed from the area of injection towards corpus callosum and brain cortex. Interestingly, passaged rat bone marrow stromal cells expressed NGF and GDNF mRNA. CONCLUSIONS: The bone marrow cells could be successfully transplanted to the brain either for the purpose of trans-differentiation, or for the expression of desired growth factors.
Assuntos
Células da Medula Óssea/citologia , Transplante de Tecido Encefálico , Encéfalo/patologia , Sobrevivência de Enxerto , Neurônios/metabolismo , Transplante de Células-Tronco , Adenoviridae , Animais , Antígenos CD/metabolismo , Biomarcadores/metabolismo , Regulação da Expressão Gênica , Leucócitos Mononucleares/virologia , Masculino , Fatores de Crescimento Neural/genética , Fatores de Crescimento Neural/metabolismo , Ácido Quinolínico , Ratos , Ratos Sprague-Dawley , Coloração e Rotulagem , Fatores de TempoRESUMO
El presente estudio prospectivo, de pesquisa, a 2 anos, investiga la relacion entre los procedimientos endoscopicos que se utilizan en el I.G.B.J. en los pacientes con H.D.A. por lesiones no varicosas como son la inyeccion endoscopica y la implantacion de clips, para lo cual utilizamos como drogas esclerosantes el alcohol absoluto, adrenalina o su combinacion y clips Olympus MD 850. El informe preliminar a dos anos reporta la esclerosis de 55 pacientes, a 8 se le aplicaron clips y en 3 se realizaron procedimientos combinados. No encontramos diferencias significativas entre los procedimientos ni entre los esclerosantes; las tasas de complicaciones, resangrado, cirugias y defunciones se encuentran dentro de los limites descritos por las distintas series, con un descenso marcado en el numero de cirugias por la enfermedad en comparacion a 1984.
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Hemorragia Gastrointestinal/terapia , Hemostase Endoscópica/métodos , Hemostase Endoscópica/reabilitação , Resultado do TratamentoRESUMO
El presente estudio prospectivo de pesquisa a 2 años, investiga la relación entre los procedimientos endoscópicos que se utilizan en el I.G.B.J. en los pacientes con H.D.A. por lesiones no varicosas como son la infección endoscópica y la implantación de clips, para lo cual utilizamos como drogas esclerosantes el alcohol absoluto, la adrenalina o su combinación y clips olympus MD 850. El informe preliminar reporta la esclerosis de 21 pacientes y a 2 se le aplicaron clips. no encontramos diferencias significativas entre los procedimientos, ni entre los esclerosantes, las tasas de complicaciones, resangrado, cirugías y difunciones se encuentran dentro de los límites descritos por las distintas series, con un descenso marcado en el número de cirugías por la enfermedad en comparación a 1984.