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Prolonged survival and expression of neural markers by bone marrow-derived stem cells transplanted into brain lesions
Alberti, Esteban; Los, MareK; García, Rocio; Fraga, Jorge L; Serrano, Teresa; Hernández, Elizabeth; Klonisch, Thomas; Macias, René; Martínez, Lisis; Castillo, Lázara; Cuétara, Karelys de la.
Afiliação
  • Alberti, Esteban; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Los, MareK; Deparment of Human Anatomy and Cell Science. Univ Manitoba. Winnipeg. Canadá
  • García, Rocio; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Fraga, Jorge L; Department of Parasitology. Pedro Kouri Institute. Havana. Cuba
  • Serrano, Teresa; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Hernández, Elizabeth; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Klonisch, Thomas; Deparment of Human Anatomy and Cell Science. Univ Manitoba. Winnipeg. Canadá
  • Macias, René; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Martínez, Lisis; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Castillo, Lázara; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
  • Cuétara, Karelys de la; Department of Neurobiology. Internationl Center of Neurological Restotation (CIREN). Havana. Cuba
Med Sci Monit ; 15(2)Feb. 2009. ilus, tab, graf
Article em En | CUMED | ID: cum-39786
Biblioteca responsável: CU1.1
ABSTRACT
BACKGROUND: Bone marrow-derived stem cell transplantation is a potentially viable therapeutic option for the treatment of neurodegenerative disease. MATERIAL/METHODS: We have isolated bone marrow stem cells by standard method. We then evaluated the survival of rats' bone marrow mononuclear cells implanted in rats' brain. The cells were extracted from rats' femurs, and marked for monitoring purposes by adenoviral transduction with Green Fluorescent Protein (GFP). Labeled cells were implanted within the area of rats' striatum lesions that were induced a month earlier employing quinolinic acid-based method. The implants were phenotyped by monitoring CD34; CD38; CD45 and CD90 expression. Bone marrow stromal cells were extracted from rats' femurs and cultivated until monolayer bone marrow stromal cells were obtained. The ability of bone marrow stromal cells to express NGF and GDNF was evaluated by RT-PCR. RESULTS: Implanted cells survived for at least one month after transplantation and dispersed from the area of injection towards corpus callosum and brain cortex. Interestingly, passaged rat bone marrow stromal cells expressed NGF and GDNF mRNA. CONCLUSIONS: The bone marrow cells could be successfully transplanted to the brain either for the purpose of trans-differentiation, or for the expression of desired growth factors(AU)
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Texto completo: 1 Coleções: 06-national / CU Base de dados: CUMED Assunto principal: Encéfalo / Células da Medula Óssea / Transplante de Tecido Encefálico / Transplante de Células-Tronco / Sobrevivência de Enxerto / Neurônios Limite: Animals Idioma: En Revista: Med Sci Monit Ano de publicação: 2009 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
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Texto completo: 1 Coleções: 06-national / CU Base de dados: CUMED Assunto principal: Encéfalo / Células da Medula Óssea / Transplante de Tecido Encefálico / Transplante de Células-Tronco / Sobrevivência de Enxerto / Neurônios Limite: Animals Idioma: En Revista: Med Sci Monit Ano de publicação: 2009 Tipo de documento: Article