RESUMO
Fundamento. La asociación bidireccional entre factores psicosociales y afecciones alérgicas ha sido abordada históricamente con resultados poco concluyentes y el número de estudios que la abordan es escaso en Argentina. Objetivo. Valorar la asociación entre prevalencia de enfermedades alérgicas y variables del contexto de la salud mental. Lugar de aplicación. Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Argentina. Diseño. Estudio observacional de corte transversal. Población. Se integró con 3.183 adultos jóvenes, 66,3% mujeres, entre 17 y 36 años. Método. Se utilizaron cuestionarios para enfermedades alérgicas y factores psicológicos y familiares. Resultados. Se observó ansiedad significativa en asociación con asma, rinitis, eccema, urticaria y angioedema. Se halló dificultad para identificar y distinguir sentimientos de sensaciones corporales y para describir sentimientos en los individuos sintomáticos. Se encontró mayor frecuencia de padres ausentes entre individuos que manifestaban asma o rinitis y de primogénitos en sujetos que referían rinitis. Todas las enfermedades se asociaron a la presencia de antecedentes familiares y al tabaquismo. Conclusión. La significativa asociación entre factores psicológicos y alergia agrega evidencias para sugerir la necesidad del abordaje interdisciplinario de alergia tanto en el campo de la investigación fisiopatológica o epidemiológica como en el terreno de la promoción, prevención y atención de la salud integral de las personas(AU)
Background. Bidirectional relationship between psychosocial factors and allergic processes has been historically assessed but remain inconclusive and not much explored in Argentine. Objective. To asses associations between allergic conditions prevalence and some factors related to mental health. Setting. Medical Sciences Faculty, National University of Rosario, Argentina. Design. Observational cross sectional study. Population. 3183 young adults, 66,3% female aged 17 to 36 years. Methods. Statement with different instruments for the symptomatic exploration of allergy and some psychological and familiar factors. Results. Significant anxiety in association with asthma, rhinitis, eczema, urticaria and angioedema were observed. Difficult to identify and distinguish feelings from corporal sensations and to describe feelings were observed in all allergic conditions. Individual which had shown asthma and rhinitis had more frequently the absence of father alive. Among rhinitis subjects the first order of birth was significantly observed. All diseases were associated with the presence of family history and smoking. Conclusion. Significant association between psychological factors and allergic condition were shown. Factors analyzed were related to genetic heritage as much as individual development in familiar and sociocultural environment. They constitute evidence enough to suggest the need of the interdisciplinary boarding of allergy so much at the field of the physiopathologic or epidemiologic investigation as in the ground of promotion, prevention and attention of the people comprehensive health.(AU)
Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Ansiedade , Saúde Mental , Hipersensibilidade , Asma , Urticária , Rinite , Eczema , AngioedemaRESUMO
Neonatal red cells (umbilical cord blood) were in vitro incubated in isotonic media (thiocyanate as predominant anion). This experimental condition was selected as it was known that this chaotropic anion induced activation of Na+-H+ exchange. The transport amiloride-sensitive mechanism, that decreased the acidic intracellular pH change occurring in this medium, would correspond to Na+-H+ exchange (NHE1 isoform). However, the Na+-Li+ exchange, also determined in the cells in the above-mentioned medium was not affected by amiloride. The present data suggest that an amiloride insensitive Na+-H+ exchange isoform would express Na+-Li+ countertransport in these cells.
Assuntos
Eritrócitos/metabolismo , Trocadores de Sódio-Hidrogênio/metabolismo , Sódio/metabolismo , Amilorida/farmacologia , Cátions Monovalentes , Células Cultivadas , Eritrócitos/efeitos dos fármacos , Humanos , Recém-Nascido , Transporte de Íons , Lítio/metabolismo , Isoformas de Proteínas/metabolismo , Fatores de TempoRESUMO
The plasma membrane Na+/Li+ exchange is of research interest because it is involved in some pathologic states. A kinetic analysis of this transport system would be of greater importance as compared to the flux determination under "standard" conditions (reactants saturating levels) as a way to define normal values. Unidirectional lithium fluxes in red blood cells from normal adults were determined as a function of "trans" (contralateral) sodium ion concentration. Relating Li+ efflux and influx (Na+ "trans") kinetic parameters we found free carrier reorientation between the two phases (asymmetry).
Assuntos
Eritrócitos/metabolismo , Lítio/metabolismo , Sódio/metabolismo , Cátions Monovalentes , Células Cultivadas , Humanos , Transporte de Íons , Cinética , Potássio/metabolismo , Espectrofotometria AtômicaRESUMO
Human red blood cells from prenatal stages of hemopoiesis (umbilical cord blood) and with increased lithium content were submitted to media with varying sodium concentrations in order to characterize extracellular sodium dependent-lithium efflux. On the other hand cells with different sodium contents were submitted to lithium media in order to characterize cellular sodium dependent-lithium influx through the above system. Experimental data were fitted by simple Michaelis-Menten kinetics. The Km value for lithium efflux (extracellular sodium-dependent) was significantly lower than the Km value for lithium influx (cellular sodium -dependent). The Vmax value for lithium influx was significantly greater than that for lithium efflux. The Vmax/Km ratio values were independent from the way in which the fluxes were measured. It is concluded that the simple carrier cannot be excluded as a model of the Na+-Li+ exchange system in these cells.
Assuntos
Eritrócitos/citologia , Eritrócitos/enzimologia , Hematopoese/fisiologia , Lítio/farmacocinética , Sódio/farmacocinética , Adulto , Fatores Etários , Transporte Biológico/fisiologia , Eritrócitos/química , Sangue Fetal/fisiologia , Humanos , Recém-Nascido , Cinética , Trocadores de Sódio-Hidrogênio/metabolismo , ATPase Trocadora de Sódio-Potássio/metabolismoRESUMO
Red cells from umbilical cord with increased lithium content were submitted to different experimental conditions in order to study lithium flux components. There appeared three components: First, an ouabain-sensitive component, related to Na+ replacement with Li+ in the primary active Na+/K+ transport system. The magnitude of this fraction is greater than in adults' red cells. Second, an outside sodium-dependent Li+ efflux fraction, corresponding to the Li+/Na+ countertransport system with Vmax and K(m) values of 0.1 (mmol/l cells.h) and 2.58 (mmol/l), respectively. The Na+o-affinity for lithium efflux in this system is greater in neonatal than in adults' red cells. Third, a leak fraction with an equal value to that reported in adults' red cells. Furthermore, the possible non-existence of a bumetanide-sensitive lithium flux fraction was shown in neonatal red cells.