RESUMO
Trypanosoma cruzi is the etiological agent of Chagas disease, whose clinical outcome ranges from asymptomatic individuals to chronic fatal megasyndromes. Despite being central to pathogenesis, the regulation of parasite virulence factors' expression remains largely unknown. In this work, the relative expression of several parasite virulence factors between two TcI strains (Ninoa, low virulence and Qro, high virulence) was assessed by qRT-PCR of total and of polysome-associated mRNA, as well as by western blots. Trypomastigotes were also incubated with specific anti-sense morpholino oligonucleotides to block the translation of a selected virulence factor, calreticulin, in both strains. Ninoa trypomastigotes showed significantly lower levels of trypomastigote-decay acceleration factor, complement regulatory protein, complement C2 receptor inhibitor trispanning, and glycoproteins 82 and 90 mRNAs compared with Qro. There was a significantly lower recruitment of complement regulatory protein and complement C2 receptor inhibitor trispanning mRNAs to polysomes and higher recruitment of MASP mRNA to monosomes in Ninoa strain. Calreticulin mRNA displayed both a higher total mRNA level and recruitment to translationally active polysomes in the Ninoa strain (low virulence) than in the Qro strain (high virulence). When calreticulin was downregulated by ≈ 50% by anti-sense morpholino oligonucleotides, a significant decrease of parasite invasion in mammalian cells was found in both strains. Calreticulin downregulation, however, only increased significantly the activation of the complement system by Ninoa trypomastigotes. These results suggest a role for the regulation of virulence factors' gene expression in the differential virulence among T. cruzi strains. Furthermore, a possible function of calreticulin in parasite invasion not related to its binding to complement factors is shown.
Assuntos
Regulação da Expressão Gênica , Genes de Protozoários/genética , Trypanosoma cruzi/genética , Trypanosoma cruzi/patogenicidade , Fatores de Virulência/genética , Virulência/genética , Animais , Western Blotting , Calreticulina/genética , Doença de Chagas/parasitologia , Chlorocebus aethiops , Cobaias , RNA Mensageiro/metabolismo , Células VeroRESUMO
Objetivos: Comparar la recesión clase II de Miller en el post test entre el tratamiento combinado de injerto libre de tejido conectivo y la técnica de Allen con el injerto libre de tejido conectivo. Material y métodos: Para lo cual se conformaron dos grupos de estudio, uno experimental y otro control, en los cuales se midieron y evaluaron las recesiones, el tamaño de los grupos se determinaron mediante formula y la asignación de las unidades de estudio a los grupos fue intencional. Para el procesamiento y análisis de los datos se empleó la estadística descriptiva mediante frecuencias absolutas y relativas y la estadística inferencial a través del chi2, igualmente se utilizó ANOVA para medidas repetidas y para la comparación final de los postest se utilizó T de muestras independientes. Resultados: Se encontró diferencia estadística significativa en las medidas de la recesión vertical entre los últimos pos test de ambos grupos experimental y control. Conclusiones: Se rechaza la hipótesis nula y se acepta la hipótesis alterna donde se refiere a que exista diferencia en la recesión gingival clase II de Miller entre el grupo experimental y control en pacientes de consulta privada, con una significancia de 0,05. (AU)
Objectives: to compare the recession class II of miller in the post test and the combined treatment of free connective tissue graft technique of Allen with free connective tissue graft. Material and Methods: For which formed two groups of study, one pilot and another control, which were measured and assessed the recessions, the size of the groups were determined by formula and the allocation of units of study groups was intentional. For the processing and analysis of the data was used by absolute and relative frequencies descriptive statistics and inferential statistics through the chi2, also used ANOVA for repeated measurements and T independent samples was used for the final comparison of the posttest. Results: There is significant statistical difference in measures of vertical recession among the last pos experimental test between the two groups and control, therefore the null hypothesis is rejected and accepted the alternate hypothesis. Conclusions: There is difference in gingival recession class Miller II between the experimental group and control in patients of private consultation with a 0.05 significance. (AU)
Assuntos
Humanos , Transplante de Tecidos , Tecido Conjuntivo , Retração Gengival/terapiaRESUMO
Introducción: La presente investigación tuvo por objeto determinar la eficacia de la cefalexina, la terramicina y del ácido cítrico como bioacondicionadores en los niveles de cobertura radicular y de inserción en pacientes con recesión gingival clase II de Miller intervenidos a colgajo desplazado coronalmente. Materiales y método: Se trata de un ensayo clínico randomizado intergrupo, con pretest y postest múltiple, se conformaron 3 grupos: dos experimentales (1 y 2) que recibieron respectivamente la cefalexina y la terramicina; y un grupo control en el que se le aplicó ácido cítrico, como bioacondicionadores cementarios. Cada grupo estuvo constituido por 20 recesiones gingivales clase II de Miller, con indicación básica de colgajo desplazado coronalmente, los datos se evluaron mediante las pruebas Chi Cuadrado de Pearson y ANOVA. Resultados: No hubo diferencia estadística significativa en el efecto de la cefalexina, la terramicina y en el ácido cítrico en el nivel de cobertura radicular a los 30 días. El contraste ANOVA mostró en cambio, a los 60 días una diferencia estadística significativa, en el efecto de dichos bioacondicionadores en la ganancia de inserción. Conclusiones:El uso de bioacondicionares genera resultados significativos en el manejo de recesiones gingivales, siendo más afectivos a largo plazo. (AU)
Introduction: The present study was designed to determine the efficacy of cefalexin, the terramycin and citric acid as bioconditioners in root coverage and levels of insertion in patients with class II Miller gingival recession intervened to displaced face flap. Materials and method: is of a trial clinical randomized Intergroup, with pretest and posttest multiple, is formed 3 groups: two experimental (1 and 2) that received respectively the cephalexin and the terramycin; and a group control in which is it applied acid citric, as bioconditioners inserted. Each group was made up of 20 gingival recessions class Miller II, with basic indication of flap displaced face, the data is evluaron using ANOVA and Pearson Chi square tests. Results: There was No statistical significant difference in the effect of cefalexin, the terramycin and citric acid at the level of root coverage within 30 days. The contrast ANOVA showed instead, to them 60 days a difference statistical significant, in the effect of such bioconditioners in the gain of inclusion. Conclusions: the use of bioconditioners generates results significant at the handling of recessions gingival, being more affec tive to long term. (AU)