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Differential gene expression of virulence factors modulates infectivity of TcI Trypanosoma cruzi strains.
Arroyo-Olarte, Ruben D; Martínez, Ignacio; Lujan, Eduardo; Mendlovic, Fela; Dinkova, Tzvetanka; Espinoza, Bertha.
Afiliação
  • Arroyo-Olarte RD; Instituto de Investigaciones Biomédicas, Departamento de Inmunología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
  • Martínez I; Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360, Mexico City, Mexico.
  • Lujan E; Instituto de Investigaciones Biomédicas, Departamento de Inmunología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
  • Mendlovic F; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
  • Dinkova T; Facultad de Medicina, Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
  • Espinoza B; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
Parasitol Res ; 119(11): 3803-3815, 2020 Nov.
Article em En | MEDLINE | ID: mdl-33006041
Trypanosoma cruzi is the etiological agent of Chagas disease, whose clinical outcome ranges from asymptomatic individuals to chronic fatal megasyndromes. Despite being central to pathogenesis, the regulation of parasite virulence factors' expression remains largely unknown. In this work, the relative expression of several parasite virulence factors between two TcI strains (Ninoa, low virulence and Qro, high virulence) was assessed by qRT-PCR of total and of polysome-associated mRNA, as well as by western blots. Trypomastigotes were also incubated with specific anti-sense morpholino oligonucleotides to block the translation of a selected virulence factor, calreticulin, in both strains. Ninoa trypomastigotes showed significantly lower levels of trypomastigote-decay acceleration factor, complement regulatory protein, complement C2 receptor inhibitor trispanning, and glycoproteins 82 and 90 mRNAs compared with Qro. There was a significantly lower recruitment of complement regulatory protein and complement C2 receptor inhibitor trispanning mRNAs to polysomes and higher recruitment of MASP mRNA to monosomes in Ninoa strain. Calreticulin mRNA displayed both a higher total mRNA level and recruitment to translationally active polysomes in the Ninoa strain (low virulence) than in the Qro strain (high virulence). When calreticulin was downregulated by ≈ 50% by anti-sense morpholino oligonucleotides, a significant decrease of parasite invasion in mammalian cells was found in both strains. Calreticulin downregulation, however, only increased significantly the activation of the complement system by Ninoa trypomastigotes. These results suggest a role for the regulation of virulence factors' gene expression in the differential virulence among T. cruzi strains. Furthermore, a possible function of calreticulin in parasite invasion not related to its binding to complement factors is shown.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma cruzi / Virulência / Regulação da Expressão Gênica / Genes de Protozoários / Fatores de Virulência Limite: Animals Idioma: En Revista: Parasitol Res Assunto da revista: PARASITOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma cruzi / Virulência / Regulação da Expressão Gênica / Genes de Protozoários / Fatores de Virulência Limite: Animals Idioma: En Revista: Parasitol Res Assunto da revista: PARASITOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha