RESUMO
BACKGROUND: Epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) is the optimal treatment for EGFR-mutant advanced non-small cell lung cancer (NSCLC). However, most patients developed systemic or local progression due to acquired EGFR-TKI resistance. This retrospective study aimed to evaluate the feasibility of continued EGFR-TKI with concurrent radiotherapy (CTCRT) in patients with local progression after front-line EGFR-TKI treatment. METHODS: Advanced NSCLC patients with active EGFR mutation who received EGFR-TKI were treated with CTCRT after local progression. Medical data were analyzed for time to progression (TTP), progression-free survival (PFS), tumor response rate, overall survival (OS) and adverse events. RESULTS: A total of 50 irradiated lesions from 44 patients were included. Median TTP and PFS of measurable lesions (n = 31) were both significantly prolonged after local radiotherapy (TTP1 + TTP2 vs. TTP1: 21.7 vs. 16.0 months, P = 0.010; PFS1 + PFS2 vs. PFS1: 21.3 vs. 16.0 months, P = 0.027). For all lesions (n = 50), objective response rate (ORR) and local tumor control rate (LCR) were 54.0 and 84.0%, respectively. Median OS was 26.6 months. There were no serious adverse events before or after radiotherapy. CONCLUSIONS: The treatment modality of CTCRT is considerable and effective for EGFR-mutant NSCLC patients even with local failure from front-line EGFR-TKI treatment.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Quimiorradioterapia/métodos , Neoplasias Pulmonares/terapia , Inibidores de Proteínas Quinases/uso terapêutico , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Quimiorradioterapia/efeitos adversos , Éteres de Coroa/administração & dosagem , Éteres de Coroa/efeitos adversos , Progressão da Doença , Intervalo Livre de Doença , Receptores ErbB/antagonistas & inibidores , Cloridrato de Erlotinib/administração & dosagem , Cloridrato de Erlotinib/efeitos adversos , Feminino , Gefitinibe , Humanos , Estimativa de Kaplan-Meier , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/patologia , Recidiva Local de Neoplasia/terapia , Quinazolinas/administração & dosagem , Quinazolinas/efeitos adversos , Estudos Retrospectivos , Terapia de Salvação/métodosRESUMO
BACKGROUND: Methylenetetrahyfrofolate reductase (MTHFR) is the key enzyme for one carbon and folate metabolism. Previous studies have drawn different conclusions about the relationship between the mutation of MTHFR and hepatocellular carcinoma (HCC) occurrence. MTHFR polymorphisms' influence on liver transplantation for HCC recurrence has yet not been reported. Aim of this study was to clarify the impact of MTHFR polymorphism on hepatocarcinogenesis and the prognosis of liver transplant recipient with HCC. METHODS: This study enrolled 244 HCC patients and 487 healthy individuals in Chinese Han population to analyze the influence of MTHFR polymorphism on HCC susceptibility first. Furthermore, this research choose another 100 donors' and 104 recipients' specimens to detect the association between polymorphism of MTHFR and post-transplant HCC recurrence. RESULT: rs1801131 polymorphism A to C was associated with the occurrence of HCC in Chinese Han population (p < 0.05), especially in age exceeding 50 years (p < 0.01). No association was observed with rs1801133 polymorphism and HCC occurrence. The mean tumor-free survival for recipients with donor liver graft rs1801133 C to T variants was shorter than CC type (12.63 ± 3.84 vs 22.43 ± 4.74 months, p < 0.05). Multivariate analysis revealed that Donor rs1801133 and Hangzhou criteria were two independent prognostic factors for tumor-free survival (p < 0.05). Neither donor rs1801131 polymorphism nor recipients' MTHFR polymorphisms was associated with HCC recurrence. CONCLUSION: This study demonstrated that MTHFR polymorphism was associated with HCC occurrence and post-transplant HCC recurrence. rs1801131 mutation A to C is a valuable molecular biomarker for predicting HCC occurrence in Chinese Han population. Donor MTHFR rs1801133 C to T polymorphism could present as a promising prognostic biomarkers for HCC recurrence in liver transplant recipients.
Assuntos
Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/genética , Transplante de Fígado/mortalidade , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Recidiva Local de Neoplasia/genética , Adulto , Idoso , Povo Asiático/genética , Biomarcadores Tumorais/genética , Carcinoma Hepatocelular/cirurgia , Intervalo Livre de Doença , Feminino , Predisposição Genética para Doença/genética , Genótipo , Humanos , Estimativa de Kaplan-Meier , Neoplasias Hepáticas/cirurgia , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Prognóstico , Modelos de Riscos Proporcionais , Resultado do TratamentoRESUMO
We report the case of a 45-year-old woman with a painless soft tissue mass on the left side of her neck. At surgery, the lesion was solid and rubbery and revealed gray-white fibrous areas. It was pathologically confirmed as an elastofibroma. Elastofibroma is a rare, benign soft tissue tumour that is most commonly encountered in patients more than 55 years of age. Elastofibroma arising from connective tissue and usually found in the subscapular region is extremely rare in the neck. The pathogenesis of elastofibroma is still unclear but increased mechanical stress or nutritional derangement consequent to vascular involvement and hereditary factors may predispose to this lesion. Marginal excision is the treatment of choice in symptomatic patients.
RESUMO
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
Assuntos
Animais , Feminino , Humanos , Antibióticos Antineoplásicos/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Doxorrubicina/uso terapêutico , Resistencia a Medicamentos Antineoplásicos/genética , Inativação Gênica , Peptídeos e Proteínas de Sinalização Intracelular/genética , Western Blotting , Neoplasias da Mama/genética , Carcinoma/tratamento farmacológico , Carcinoma/genética , Modelos Animais de Doenças , Genes MDR , Vetores Genéticos/genética , Inibidores do Crescimento/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Lentivirus/genética , Camundongos Endogâmicos BALB C , Camundongos Nus , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/efeitos dos fármacos , Interferência de RNA , RNA Interferente Pequeno/genética , /efeitos dos fármacosRESUMO
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
Assuntos
Antibióticos Antineoplásicos/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Doxorrubicina/uso terapêutico , Resistencia a Medicamentos Antineoplásicos/genética , Inativação Gênica , Peptídeos e Proteínas de Sinalização Intracelular/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/efeitos dos fármacos , Animais , Western Blotting , Neoplasias da Mama/genética , Carcinoma/tratamento farmacológico , Carcinoma/genética , Modelos Animais de Doenças , Feminino , Genes MDR , Vetores Genéticos/genética , Inibidores do Crescimento/genética , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Lentivirus/genética , Células MCF-7 , Camundongos Endogâmicos BALB C , Camundongos Nus , Interferência de RNA , RNA Interferente Pequeno/genética , Proteína Supressora de Tumor p53/efeitos dos fármacosRESUMO
Although the efficacy and safety of the Sargenti technique have remained controversial for more than a decade, the technique still is widely used by many dentists throughout the world. The following report describes a retrospective analysis of 367 cases that looked at the contribution of instrumentation technique and fill adequacy to paste dissolution.
Assuntos
Eugenol/farmacocinética , Formaldeído/farmacocinética , Materiais Restauradores do Canal Radicular/farmacocinética , Óxido de Zinco/farmacocinética , Adulto , Cavidade Pulpar/metabolismo , Combinação de Medicamentos , Eugenol/efeitos adversos , Eugenol/química , Extravasamento de Materiais Terapêuticos e Diagnósticos , Feminino , Formaldeído/efeitos adversos , Formaldeído/química , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Materiais Restauradores do Canal Radicular/efeitos adversos , Materiais Restauradores do Canal Radicular/química , Obturação do Canal Radicular/efeitos adversos , Tratamento do Canal Radicular/efeitos adversos , Tratamento do Canal Radicular/instrumentação , Solubilidade , Óxido de Zinco/efeitos adversos , Óxido de Zinco/químicaRESUMO
The type of radio-frequency (RF) coil known as a high-pass birdcage consists of a set of N; wires arranged axially on the surface of a cylinder and connected by capacitors at each end. Such coils are widely used for NMR imaging because of the high degree of field homogeneity they provide. It is shown how to create homogeneous fields at two frequencies, using an unequal distribution of capacitance. A theoretical analysis which uses the discrete Fourier transform of the currents with respect to the angular positions of the N; wires is presented. A perturbation theory analysis indicates a small sacrifice in homogeneity. The root-mean-square deviation of field magnitude around a circle is proposed as a measure of field inhomogeneity. For the case of double resonance at proton and fluorine frequencies, the loss of homogeneity is at worst 1% and is small compared to the natural inhomogeneity for an N=8 wire coil for radii up to one half the coil radius. The presence of a conducting shield degrades the homogeneity. The theoretical ideas were confirmed in a computer simulation of one particular coil design. A working coil was constructed and images obtained of proton and fluorine phantoms.