CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;47(4): 273-278, 8/4/2014. graf
Article
em En
| LILACS
| ID: lil-705769
Biblioteca responsável:
BR1.1
ABSTRACT
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Neoplasias da Mama
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Doxorrubicina
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Resistencia a Medicamentos Antineoplásicos
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Inativação Gênica
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Peptídeos e Proteínas de Sinalização Intracelular
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Antibióticos Antineoplásicos
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
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MEDICINA
Ano de publicação:
2014
Tipo de documento:
Article
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Project document
País de afiliação:
China
País de publicação:
Brasil