CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo

Wang, X.M.; Gao, S.J.; Guo, X.F.; Sun, W.J.; Yan, Z.Q.; Wang, W.X.; Xu, Y.Q.; Lu, D.

Wang, X.M.; Gao, S.J.; Guo, X.F.; Sun, W.J.; Yan, Z.Q.; Wang, W.X.; Xu, Y.Q.; Lu, D..

Afiliação

Wang, X.M.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Gao, S.J.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Guo, X.F.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Sun, W.J.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Yan, Z.Q.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Wang, W.X.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Xu, Y.Q.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN
Lu, D.; Harbin Medical University. The Second Affiliated Hospital. Department of Oncology. Harbin. CN

Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;47(4): 273-278, 8/4/2014. graf

Article em En | LILACS | ID: lil-705769

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.

Assuntos

Animais; Feminino; Humanos; Antibióticos Antineoplásicos/uso terapêutico; Neoplasias da Mama/tratamento farmacológico; Doxorrubicina/uso terapêutico; Resistencia a Medicamentos Antineoplásicos/genética; Inativação Gênica; Peptídeos e Proteínas de Sinalização Intracelular/genética; Western Blotting; Neoplasias da Mama/genética; Carcinoma/tratamento farmacológico; Carcinoma/genética; Modelos Animais de Doenças; Genes MDR; Vetores Genéticos/genética; Inibidores do Crescimento/genética; Peptídeos e Proteínas de Sinalização Intracelular/metabolismo; Lentivirus/genética; Camundongos Endogâmicos BALB C; Camundongos Nus; Membro 1 da Subfamília B de Cassetes de Ligação de ATP/efeitos dos fármacos; Interferência de RNA; RNA Interferente Pequeno/genética; /efeitos dos fármacos

Palavras-chave

Breast neoplasms; CIAPIN1 gene; MDR1 gene; Multidrug resistance; RNA interference

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Neoplasias da Mama / Doxorrubicina / Resistencia a Medicamentos Antineoplásicos / Inativação Gênica / Peptídeos e Proteínas de Sinalização Intracelular / Antibióticos Antineoplásicos Limite: Animals / Female / Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2014 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil

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