RESUMO
Molecular techniques were adopted to diagnose rabies viral RNA from skin biopsy samples collected from 20 animals. Nested RT-PCR and TaqMan real time PCR techniques have successfully diagnosed rabies viral RNA in 9 and 11 skin samples, respectively. The sensitivity of both techniques was calculated in comparison to FAT applied on brain samples. Sensitivity of 76.47% was obtained with nested RT-PCR on skin biopsy samples while Taqman real time PCR revealed sensitivity of 92.85%. It was concluded that TaqMan real time PCR is a useful, specific, sensitive and better molecular approach for antemortem rabies diagnosis from skin samples of rabies suspected animals.
RESUMO
The present study aimed at evaluating diagnostic accuracy and efficacy of cytology for rapid diagnosis and differentiation of various mammary affections of canines and the comparison of cytological findings with that of histopathology. Cytological evaluation was conducted on 33 dog patients having mammary gland lesions. Out of these, 28 cases were diagnosed for various mammary affections and rest five cases were inconclusive on the basis of cytological evaluation. Among various affections, 23 were diagnosed as tumors, four as mastitis and one as a case of steatitis. Comparison of cytological findings with histological diagnoses yielded 70% concordance between the two. For malignant mammary tumours, the correlation was 92.30% while for benign mammary tumours, it was quite low (66.66%). Among malignant tumours (n=13), there was one false positive case (7.69%) and one false negative case (7.69%). One case of mixed mammary tumour was misdiagnosed as simple adenocarcinoma. In benign tumours, only one false negative (3.3%) result was found and there was no false positive result. Various cytological techniques like touch impression, fine needle biopsy and teat fluid cytology were employed and these techniques yielded sensitivity and specificity of cytological diagnoses as 87.5% and 85.7%, respectively. The positive and negative predictive value of cytology for diagnosing mammary tumou