Antemortem diagnosis of rabies from skin: comparison of nested RT-PCR with TaqMan real time PCR
Braz. J. Vet. Pathol.
; 5(3): 116-119, 2012.
Article
em En
| VETINDEX
| ID: vti-689810
Biblioteca responsável:
BR68.1
ABSTRACT
Molecular techniques were adopted to diagnose rabies viral RNA from skin biopsy samples collected from 20 animals. Nested RT-PCR and TaqMan real time PCR techniques have successfully diagnosed rabies viral RNA in 9 and 11 skin samples, respectively. The sensitivity of both techniques was calculated in comparison to FAT applied on brain samples. Sensitivity of 76.47% was obtained with nested RT-PCR on skin biopsy samples while Taqman real time PCR revealed sensitivity of 92.85%. It was concluded that TaqMan real time PCR is a useful, specific, sensitive and better molecular approach for antemortem rabies diagnosis from skin samples of rabies suspected animals.
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Base de dados:
VETINDEX
Idioma:
En
Revista:
Braz. J. Vet. Pathol.
Ano de publicação:
2012
Tipo de documento:
Article