RESUMO

A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in beta-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential beta-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.

Assuntos

Actinas/genética , Adaptação Fisiológica , Carpas/fisiologia , Transcrição Gênica/fisiologia , Animais , Sequência de Bases , Primers do DNA , Hibridização In Situ , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Ribossômico/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa