Environmental acclimatization of the carp modulates the transcription of beta-actin.
J Cell Biochem
; 80(2): 223-8, 2000 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-11074593
A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in beta-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential beta-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Carpas
/
Adaptação Fisiológica
/
Actinas
Limite:
Animals
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Estados Unidos