RESUMO
PURPOSE: The aim of this study was to analyze and compare the expression of osteocalcin, RANK-L, osteoprotegerin, in sinus lift procedures using bovine hydroxyapatite (HA). MATERIAL AND METHODS: Twenty four male rabbits underwent bilateral sinus lift procedures were distributed into two groups, according to the sinus filling material: Group 1) autogenous bone graft; and Group 2) bovine HA. All groups were sacrificed after 14 and 30 days, for microscopic and immunohistochemistry analysis. RESULTS: At 14 days after surgery, discrete osteogenesis was observed in the highly vascularized granulation tissue surrounding HA particles, as well as woven bone deposition on the biomaterial surface. Following 30 days, well organized bone trabeculas were seen surrounding the HA granules presenting areas of osteogenic activity. Morphometric findings did not show remarkable differences between groups. Bovine HA induced similar osteocalcin, RANK-L, osteoprotegerin immunoexpressivity when compared to autogenous bone graft group for both periods evaluated in this setting. CONCLUSION: Taken together, these data are consistent with the notion that HA has a similar biological behavior to autogenous bone graft in sinus lift of rabbits.
Assuntos
Matriz Óssea/transplante , Substitutos Ósseos/uso terapêutico , Minerais/uso terapêutico , Osteocalcina/análise , Osteoprotegerina/análise , Ligante RANK/análise , Levantamento do Assoalho do Seio Maxilar/métodos , Animais , Densidade Óssea/fisiologia , Transplante Ósseo/métodos , Calcificação Fisiológica/fisiologia , Bovinos , Tecido de Granulação/patologia , Masculino , Osteogênese/fisiologia , Coelhos , Fatores de Tempo , Transplante AutólogoRESUMO
Introdução: Na odontologia, a reabsorção óssea alveolar é fator limitante no bem estar dos indivíduos interferindo diretamente no sistema estomatognático, acarretando problemas no âmbito de saúde em geral. Com o objetivo de promover modalidades biológicas que possam estimular a regeneração óssea, várias estratégias biomiméticas têm sido desenvolvidas recorrendo à utilização dos mais diversos materiais possíveis à matriz óssea, culminando com o desenvolvimento de técnicas que promovam tal reparo. Objetivo: Este trabalho consiste no estudo comparativo do desempenho de filmes confeccionados com látex como membrana oclusiva em procedimento de Regeneração Óssea Guiada (ROG) em 3 preparações: Látex preservado em amônia, Látex produzidos por seringueiras dos clones IAN873 e PR255 polimerizados logo após a coleta e sem uso de amônia como conservante. Métodos: Foram utilizados 60 ratos Wistar, divididos randomicamente em 4 grupos de 15 animais, nos quais defeitos ósseos de tamanho crítico (8mm de diâmetro) foram confeccionados cirurgicamente na calvária. O grupo A foi tratado por ROG através da membrana de látex preservada em amônia, o grupo B recebeu a membrana do clone IAN873, o grupo C, a membrana do clone PR255 e o grupo D, não foi tratado por ROG. Após o período de 7, 15 e 50 dias, 5 animais de cada grupo foram eutanasiados, e as peças contendo o defeito ósseo coletadas para análise microscópica (histológica descritiva e histomorfometria). Resultados: Os resultados demonstraram que após 50 dias, houve formação óssea em maiores proporções no grupo D (p<0.05, ANOVA seguido de Tukey), sugerindo que novos experimentos devem ser realizados para se concluir a respeito da presença da amônia e a influência da espécie de seringueira.
Introduction: In dentistry, alveolar bone resorption is a limiting factor in the well being of individuals directly interfering in the stomatognathic system, causing problems in the context of overall health. Aiming to promote biological methods that can stimulate bone regeneration, several biomimetic strategies have been developed by the use of diverse materials possible to the bone matrix, culminating in the development of techniques that promote such repair. Objective: This work is a comparative study of the performance of films made with latex as occlusive membrane for Guided Bone Regeneration (GBR) procedure in three preparations: Latex preserved in ammonia, produced by Latex rubber clones IAN873 and PR255 polymerized immediately after collection and without use ammonia as a preservative. Methods: Sixty Wistar rats were randomly divided into 4 groups of 15 animals in which bone defects of critical size (8mm diameter) were made surgically in the skull. Group A was treated by GBR through the membrane látex preserved with ammonia, Group B received the membrane made of latex from IAN873, Group C, the membrane clone PR255 and group D was not treated by GBR. After a period of 7, 15 and 50 days, 5 animals from each group were euthanized, and specimens containing bone defect collected for microscopic examination (descriptive histology and histomorphometry). Results: The results showed that after 50 days there was bone formation in higher proportions in group D (p <0.05, ANOVA followed by Tukey), suggesting that further experiments should be conducted to conclude about the presence of ammonia and the influence of kind of rubber.
Assuntos
Ratos , Látex/uso terapêutico , Odontologia/tendências , Perda do Osso Alveolar/reabilitação , Regeneração ÓsseaRESUMO
PURPOSE: Considering the clinical discussion on the necessity of using a barrier membrane in the osteotomy area of sinus lift procedures to prevent fibrous tissue formation in this area and as a physical limit, the aim of this study was to analyze and compare the use of bovine hydroxyapatite (HA) with and without a biologic membrane by histopathologic analysis and immune expression of core binding factor 1 and vascular endothelium growth factor in the sinus lift in rabbits. MATERIALS AND METHODS: Sixteen male rabbits underwent bilateral sinus lift procedures and were divided into 2 groups according to the sinus filling material: group 1 received bovine HA (Bio-Oss; Geistlich Pharma AG, Wohlhusen, Switzerland) and group 2 received bovine HA and a nonporous polytetrafluorethylene membrane. All groups were sacrificed after 7, 14, 30, and 60 days for microscopic, histomorphometric, and immunohistochemical analyses. RESULTS: Microscopic analysis showed a similar bone repair pattern between the tested groups. New bone formation, soft tissue, and the remaining material were analyzed by histomorphometric analysis. No statistically significant differences (P > .05) were detected between groups for all periods analyzed. In addition, no remarkable differences were noticed in core binding factor 1 or vascular endothelium growth factor immune expression. CONCLUSION: Taken together, these results show that using a biologic membrane does not improve bone repair induced by bovine HA, as shown by histopathologic and immunohistochemical analyses.
Assuntos
Aumento do Rebordo Alveolar/métodos , Materiais Biocompatíveis/uso terapêutico , Substitutos Ósseos/uso terapêutico , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Durapatita/uso terapêutico , Maxila/cirurgia , Seio Maxilar/cirurgia , Membranas Artificiais , Fator A de Crescimento do Endotélio Vascular/análise , Animais , Matriz Óssea/transplante , Bovinos , Tecido Conjuntivo/patologia , Tecido de Granulação/patologia , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Masculino , Maxila/patologia , Seio Maxilar/patologia , Minerais/uso terapêutico , Osteogênese/fisiologia , Osteotomia/métodos , Politetrafluoretileno , Coelhos , Fatores de TempoRESUMO
OBJECTIVES: To analyze and compare the expression of core binding factor-1 (Cbfa-1)/Runx2 and vascular endothelium growth factor (VEGF) in sinus lift procedures using bovine hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP). MATERIAL AND METHODS: Twenty-four male rabbits that had undergone bilateral sinus lift procedures were divided into three groups, according to the sinus filling material: Group 1: autogenous bone graft; Group 2: bovine HA; and Group 3: beta-TCP. All groups were sacrificed after 7, 14, 30 and 60 days, for microscopic, histomorphometry and immunohistochemistry analysis. RESULTS: Microscopic analysis showed a similar bone repair pattern between the tested groups. New bone formation, soft and medular tissue, remaining material or particulate bone graft area were obtained by histomorphometric analysis. After 14 days, statistically significant differences in new bone formation were found between Group 1 (27.76+/-7.8) and Groups 2 (14.22+/-3.2) and 3 (11.1+/-7.7). After 30 days, statistically significant differences (P<0.05) were detected in bone formation between Groups 1 (31.39+/-36.5) and 2 (14.13+/-3.2). The last period showed improved bone formation in Group 2. Also, Group 2 showed higher Cbfa-1/Runx2 immunoexpression when compared with Group 3. No remarkable differences were observed in VEGF immunoexpression among groups. CONCLUSION: Taken together, both biomaterials allowed bone tissue growth in a conductive pattern and did not interfere with bone remodeling in the late period, with a slight improvement in bone tissue formation when using HA, confirmed by marked expression of Cbfa-1 at initial periods.