RESUMO
To establish if BTV was circulating in Argentina, 94 bovines from the Santo Tomé and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.
Assuntos
Vírus Bluetongue/isolamento & purificação , Bluetongue/virologia , Doenças dos Bovinos/virologia , Ceratopogonidae/virologia , Insetos Vetores/virologia , Animais , Anticorpos Antivirais/sangue , Argentina/epidemiologia , Bluetongue/epidemiologia , Bluetongue/transmissão , Vírus Bluetongue/genética , Vírus Bluetongue/imunologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/transmissão , Células Cultivadas/virologia , Galinhas , Ovos , Ensaio de Imunoadsorção Enzimática , Genoma Viral , RNA Viral/genética , Estações do Ano , Cultura de VírusRESUMO
To establish if BTV was circulating in Argentina, 94 bovines from the Santo TomÚ and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.
Assuntos
Animais , Bovinos , Bluetongue , Ceratopogonidae , Doenças dos Bovinos/virologia , Insetos Vetores , Vírus Bluetongue/isolamento & purificação , Anticorpos Antivirais , Argentina , Bluetongue , Células Cultivadas/virologia , Galinhas , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/transmissão , Ovos , Ensaio de Imunoadsorção Enzimática , Genoma Viral , RNA Viral , Estações do Ano , Vírus Bluetongue/genética , Vírus Bluetongue/imunologia , Cultura de VírusRESUMO
To establish if BTV was circulating in Argentina, 94 bovines from the Santo TomU and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.(AU)
Assuntos
Animais , Bovinos , Bluetongue/virologia , Vírus Bluetongue/isolamento & purificação , Doenças dos Bovinos/virologia , Ceratopogonidae/virologia , Insetos Vetores/virologia , Anticorpos Antivirais/sangue , Argentina/epidemiologia , Bluetongue/epidemiologia , Bluetongue/transmissão , Vírus Bluetongue/genética , Vírus Bluetongue/imunologia , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/transmissão , Células Cultivadas/virologia , Galinhas , Ovos , Ensaio de Imunoadsorção Enzimática , Genoma Viral , RNA Viral/genética , Estações do Ano , Cultura de VírusRESUMO
To establish if BTV was circulating in Argentina, 94 bovines from the Santo Tomé and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.