Lengua Azul: Aislamiento y caracterización del virus e identificación de vectores en el noreste argentino / Bluetongue: isolation and characterization of the virus and identification of vectors in northeastern Argentina
Rev. argent. microbiol
; Rev. argent. microbiol;34(3): 150-156, jul.-sept. 2002.
Article
em Es
| LILACS
| ID: lil-331790
Biblioteca responsável:
BR1.1
ABSTRACT
To establish if BTV was circulating in Argentina, 94 bovines from the Santo TomÚ and Ituzaingó Departments of Corrientes Province were sampled every 30-60 days during 14 months. Red blood cells from those animals that showed seroconvertion with a c-ELISA were processed for virus isolation by inoculation in embryonated chicken eggs and cell cultures. Cells with CPE were positive by direct and indirect immunofluorescence with BTV specific reagents. These samples examined by electron microscopy showed virus particles with BTV morphological characteristics. Blood samples and tissue culture supernantants were positive by RT-PCR technique with primers corresponding to the segment 3 of the BTV genome. Haematophagous insects were captured in one farm using light traps and Culicoides insignis Lutz was the predominant species detected. This is the first isolation of BTV in Argentina from northeastern bovines without any disease symptom.
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Doenças dos Bovinos
/
Ceratopogonidae
/
Bluetongue
/
Vírus Bluetongue
/
Insetos Vetores
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Argentina
Idioma:
Es
Revista:
Rev. argent. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Argentina