RESUMO
BACKGROUND: Ischaemia-reperfusion is one of the main causes of kidney complications. The most frequent lesion is acute tubular necrosis. Ozone oxidative preconditioning exerts a modulatory effect of redox state of renal cells in models of ischaemia-reperfusion, by stimulating endogenous antioxidant mechanisms. Similar results have been obtained in more recent studies using ischaemic postconditioning. OBJECTIVES: To evaluate the effect of ozone oxidative postconditioning on renal function and morphology in an ischaemia-reperfusion rat model. METHODS: We used forty female Wistar rats weighing between 150g-200g randomly divided into 4 groups (negative control, positive control, oxygen and ozone). The groups: positive control, oxygen and ozone were subjected to 60 minutes of ischaemia and 10 days of reperfusion. During reperfusion, the oxygen group was given 26mg/kg body weight of oxygen, and the ozone group 0.5mg/kg body weight of ozone, rectally. At the end of the experiment urine and blood samples were taken for renal function tests and kidneys were removed for histological study. RESULTS: The ozone group showed no significant differences for filtration fraction and proteinuria compared to the negative control group. The glomerular filtrate rate, renal plasma flow and creatinine showed a slight improvement in comparison with oxygen and positive control groups. The ozone group showed significantly less overall histological damage than the positive control and oxygen groups. CONCLUSIONS: Ozone postconditioning showed to have a protective effect in preserving renal function and morphology.
Assuntos
Isquemia/tratamento farmacológico , Rim/irrigação sanguínea , Ozônio/uso terapêutico , Traumatismo por Reperfusão/prevenção & controle , Administração Retal , Animais , Esquema de Medicação , Avaliação Pré-Clínica de Medicamentos , Feminino , Taxa de Filtração Glomerular , Rim/patologia , Rim/fisiopatologia , Necrose Tubular Aguda/tratamento farmacológico , Necrose Tubular Aguda/etiologia , Necrose Tubular Aguda/patologia , Necrose Tubular Aguda/prevenção & controle , Masculino , Oxigênio/administração & dosagem , Oxigênio/uso terapêutico , Ozônio/administração & dosagem , Proteinúria/etiologia , Proteinúria/prevenção & controle , Distribuição Aleatória , Ratos , Ratos Wistar , Traumatismo por Reperfusão/tratamento farmacológico , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/patologiaRESUMO
La nefropatía por inmunoglobulina A (NIgA) se reconoce como la forma más frecuente de enfermedad glomerular primaria (EGP) 1,2, .Su evolución variable3 y comúnmente prolongada, está asociada con un importante riesgo de progresión hacia la insuficiencia renal crónica terminal (IRCT) 3,4. Por este motivo se creo un grupo de colaboración entre tres hospitales de la capital cubana, con el propósito de identificar en esta entidad, marcadores de predicción evolutiva
Assuntos
Humanos , Masculino , Feminino , Adulto , Glomerulonefrite por IGA/complicações , Indicadores Básicos de Saúde , Prognóstico , Estudos RetrospectivosRESUMO
Samples of normal human endometrial tissue from eight young women were initiated in tissue culture. Four biopsies were obtained during the first phase of the cycle and four during the secretory phase. The aim of the work was to characterize ultrastructurally the possible changes of the glandular tissue without being dissociated from the stromal component. At eight hours of incubation there was preservation of all cellular components in the tissue from the first phase of the cycle whereas there were minor degenerative changes in the endometrium from the secretory phase but with outstanding preservation of intranuclear canaliculi typical of this phase. After 16 hours minimal changes were seen in proliferative endometrium while severe changes were present in secretory endometrium. The study suggests that whole endometrium in culture can be used to characterize the short time effect of drugs and substances administered during the first phase of the menstrual cycle.