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Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences.
Fernández Iglesias, A; González Núñez, L; Calunga Fernández, J L; Rodríguez Salgueiro, S; Santos Febles, E.
Afiliação
  • Fernández Iglesias A; Departamento de Investigaciones, Instituto de Nefrología Dr. Abelardo Buch, Ave. 26 y Ave. Rancho Boyeros, La Habana, Cuba. aimeefdez@infomed.sld.cu
Nefrologia ; 31(4): 464-70, 2011.
Article em En, Es | MEDLINE | ID: mdl-21738249
BACKGROUND: Ischaemia-reperfusion is one of the main causes of kidney complications. The most frequent lesion is acute tubular necrosis. Ozone oxidative preconditioning exerts a modulatory effect of redox state of renal cells in models of ischaemia-reperfusion, by stimulating endogenous antioxidant mechanisms. Similar results have been obtained in more recent studies using ischaemic postconditioning. OBJECTIVES: To evaluate the effect of ozone oxidative postconditioning on renal function and morphology in an ischaemia-reperfusion rat model. METHODS: We used forty female Wistar rats weighing between 150g-200g randomly divided into 4 groups (negative control, positive control, oxygen and ozone). The groups: positive control, oxygen and ozone were subjected to 60 minutes of ischaemia and 10 days of reperfusion. During reperfusion, the oxygen group was given 26mg/kg body weight of oxygen, and the ozone group 0.5mg/kg body weight of ozone, rectally. At the end of the experiment urine and blood samples were taken for renal function tests and kidneys were removed for histological study. RESULTS: The ozone group showed no significant differences for filtration fraction and proteinuria compared to the negative control group. The glomerular filtrate rate, renal plasma flow and creatinine showed a slight improvement in comparison with oxygen and positive control groups. The ozone group showed significantly less overall histological damage than the positive control and oxygen groups. CONCLUSIONS: Ozone postconditioning showed to have a protective effect in preserving renal function and morphology.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ozônio / Traumatismo por Reperfusão / Isquemia / Rim Tipo de estudo: Etiology_studies Limite: Animals Idioma: En / Es Revista: Nefrologia Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ozônio / Traumatismo por Reperfusão / Isquemia / Rim Tipo de estudo: Etiology_studies Limite: Animals Idioma: En / Es Revista: Nefrologia Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha