RESUMO
Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 contigs, an N50 of 179,476 bp, and 41.93% G+C content.
RESUMO
BACKGROUND: Although the FACED score has demonstrated a great prognostic capacity in bronchiectasis, it does not include the number or severity of exacerbations as a separate variable, which is important in the natural history of these patients. OBJECTIVE: Construction and external validation of a new index, the E-FACED, to evaluate the predictive capacity of exacerbations and mortality. METHODS: The new score was constructed on the basis of the complete cohort for the construction of the original FACED score, while the external validation was undertaken with six cohorts from three countries (Brazil, Argentina, and Chile). The main outcome was the number of annual exacerbations/hospitalizations, with all-cause and respiratory-related deaths as the secondary outcomes. A statistical evaluation comprised the relative weight and ideal cut-off point for the number or severity of the exacerbations and was incorporated into the FACED score (E-FACED). The results obtained after the application of FACED and E-FACED were compared in both the cohorts. RESULTS: A total of 1,470 patients with bronchiectasis (819 from the construction cohorts and 651 from the external validation cohorts) were followed up for 5 years after diagnosis. The best cut-off point was at least two exacerbations in the previous year (two additional points), meaning that the E-FACED has nine points of growing severity. E-FACED presented an excellent prognostic capacity for exacerbations (areas under the receiver operating characteristic curve: 0.82 for at least two exacerbations in 1 year and 0.87 for at least one hospitalization in 1 year) that was statistically better than that of the FACED score (0.72 and 0.78, P<0.05, respectively). The predictive capacities for all-cause and respiratory mortality were 0.87 and 0.86, respectively, with both being similar to those of the FACED. CONCLUSION: E-FACED score significantly increases the FACED capacity to predict future yearly exacerbations while maintaining the score's simplicity and prognostic capacity for death.
Assuntos
Bronquiectasia/diagnóstico , Indicadores Básicos de Saúde , Nível de Saúde , Pulmão/fisiopatologia , Adulto , Fatores Etários , Idoso , Área Sob a Curva , Argentina , Brasil , Bronquiectasia/mortalidade , Bronquiectasia/fisiopatologia , Bronquiectasia/terapia , Causas de Morte , Chile , Progressão da Doença , Dispneia/fisiopatologia , Feminino , Volume Expiratório Forçado , Hospitalização , Humanos , Pulmão/microbiologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Infecções por Pseudomonas/diagnóstico , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/isolamento & purificação , Curva ROC , Reprodutibilidade dos Testes , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/microbiologia , Fatores de Risco , Índice de Gravidade de Doença , Fatores de TempoRESUMO
Paenibacillus larvae strain MEX14 is a facultative anaerobic endospore-forming bacterium that infects Apis mellifera larvae. Strain MEX14 was isolated from domestic bee larvae collected in a backyard in Mexico City. The estimated genome size was determined to be 4.18 Mb, and it harbors 4,806 protein coding genes (CDSs).
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OBJECTIVE: To determine the profile of children and adolescents diagnosed with autism spectrum disorder (ASD) in a comprehensive care centre in Bogota, Colombia. MATERIAL AND METHODS: A descriptive-correlational study with a sample of patients who had attended the institution from 2003 to 2009. Demographic and clinical aspects were evaluated and a correlation between the diagnosis and severity being analysed in search for risk factors. RESULTS: A total of 138 patients were studied. The average age of onset was 21 months, and diagnosis had been made at 45 months. There was a male predominance (6.15:1). The predominant diagnosis was autistic syndrome (83%), followed by pervasive developmental disorder not otherwise specified (17%). There was no pathological background in the family history, during pregnancy or during the neonatal period. Motor development during the first year was normal but acquisition of language skills was compromised. The specific signs of ASD as regards the disorder in itself, communication skills, spontaneous speech, verbal comprehension, attention, imitation, use of objects, self-care and symbolic play were significantly related to the severity of the disease. CONCLUSIONS: Diagnosis of ASD is still delayed in our population and usually after referral from someone other than a health professional. The most important problems were found in communication skills and relationships with peers. The study did not show significant associated risk factors.
Assuntos
Transtornos Globais do Desenvolvimento Infantil/epidemiologia , Adolescente , Adulto , Atenção , Peso ao Nascer , Cesárea , Criança , Transtornos do Comportamento Infantil/epidemiologia , Transtornos do Comportamento Infantil/etiologia , Transtornos Globais do Desenvolvimento Infantil/psicologia , Transtornos Globais do Desenvolvimento Infantil/reabilitação , Criança Institucionalizada/psicologia , Pré-Escolar , Colômbia , Deficiências do Desenvolvimento/epidemiologia , Deficiências do Desenvolvimento/etiologia , Feminino , Humanos , Lactente , Recém-Nascido , Recém-Nascido Prematuro , Relações Interpessoais , Transtornos do Desenvolvimento da Linguagem/epidemiologia , Transtornos do Desenvolvimento da Linguagem/etiologia , Masculino , Idade Materna , Pessoa de Meia-Idade , Destreza Motora , Idade Paterna , Gravidez , Complicações na Gravidez/epidemiologia , Efeitos Tardios da Exposição Pré-Natal , Fatores de Risco , Índice de Gravidade de Doença , Adulto JovemRESUMO
Total mercury levels (Thg) were quantified in Clarias gariepinus captured from the Sagua la Grande River (Cuba) in the vicinity of an active chlor-alkali plant, and relationships among place of capture; fish size, weight, and sex; and THg levels were assessed. THg levels ranged from 67 to 375 ng/g ww in collected fish, never exceeding the Cuban recommended maximum limit for fish consumption of 500 ng/g ww. No significant correlation was observed between mercury levels and fish allometric characteristics (p < 0.05); however, levels were significantly higher in fish captured below the chlor-alkali facility, suggesting a connection between mercury bioaccumulation and plant discharges.
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Peixes-Gato/metabolismo , Mercúrio/análise , Rios/química , Poluentes Químicos da Água/análise , Animais , Tamanho Corporal , Peso Corporal , Cuba , Feminino , Geografia , Masculino , Mercúrio/farmacocinética , Mercúrio/toxicidade , Medição de Risco , Caracteres Sexuais , Poluentes Químicos da Água/farmacocinética , Poluentes Químicos da Água/toxicidadeRESUMO
This paper provides an estimate of the weekly intake of total mercury (THg) and methyl mercury (MeHg) from consumption of fish from the Sagua la Grande River, Villa Clara, Cuba, by determining the THg levels in different fish species. The levels varied between 0.143 and 0.484 µ g g(-1) on a fresh weight basis. None of the analysed fish was found to have levels above the national and international regulatory levels, although THg levels over 0.2 µ g g(-1), the threshold concentration established by the World Health Organization (WHO) for the consumption by vulnerable population groups, were found in 75% of samples. The MeHg level was found to be 84% of the THg content. A Food Frequency Survey was given to 127 townspeople to estimate river fish consumption. The weekly intake of MeHg was found to be greater than the value established by the provisional tolerable weekly intake (PTWI) in 50% of children, in 80% of pregnant women, and in 75% of women in childbearing age. These weekly intakes can represent an important risk to the population's health, especially for vulnerable groups.
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Peixes/metabolismo , Contaminação de Alimentos/análise , Mercúrio/análise , Compostos de Metilmercúrio/análise , Adolescente , Adulto , Idoso , Animais , Criança , Cuba , Ingestão de Alimentos , Monitoramento Ambiental , Feminino , Humanos , Masculino , Concentração Máxima Permitida , Mercúrio/toxicidade , Compostos de Metilmercúrio/toxicidade , Pessoa de Meia-Idade , Gravidez , Medição de Risco , Rios , Adulto JovemRESUMO
The Almendares River is central to recreational and other activities in Havana, Cuba. However, monitoring indicated significant heavy metal contamination in river sediments, especially below Calle 100, the largest landfill in Havana. This work extended previous sediment studies by determining complementary Cu, Pb, Ni, Cr, Cd, and Zn levels in indigenous water hyacinths (Eichhornia crassipes; EC) above and below the landfill. Pb, Cu, and Zn were significantly elevated in EC roots below the landfill and also correlated with sediment data (p < 0.05), implying elevated levels likely result from landfill activity and might be useful biomonitors as river remediation proceeds.
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Eichhornia/química , Metais Pesados/análise , Rios/química , Poluentes Químicos da Água/análise , Monitoramento AmbientalRESUMO
A denitrifying upflow anaerobic sludge blanket (UASB) reactor was operated at different nitrate loading rates at a C/N ratio of 1.2, with acetate as an electron donor. This resulted in an increase in the accumulation of nitrite. After this, the UASB reactor was supplemented with 100 mg NH4+-Nl(-1) d(-1), while acetate was gradually limited in the medium. This prevented nitrite accumulation at a C/N ratio of 0.6 due to an enhanced nitrite reduction rate achieved in the reactor. An increasing amount of ammonium was consumed when the C/N ratio was lowered in the medium. This suggested that ammonium was used as an alternative electron donor during denitrification, which is supported by nitrogen balances. Nitrite was shown to be toxic for the nitrogen removal process at 200-400 mg NO2--N(l(-1) when the C/N ratio was decreased to 0.4 leading to formation of ammonium. The present study showed that addition of ammonium as an alternative electron donor for denitrification achieved a nitrogen removal process with negligible accumulation of undesirable intermediates.
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Acetatos/metabolismo , Nitritos/análise , Nitrogênio/metabolismo , Compostos de Amônio Quaternário/metabolismo , Eliminação de Resíduos Líquidos/métodos , Anaerobiose , Reatores Biológicos , Carbono/metabolismo , Temperatura Alta , Nitratos/análise , Oxirredução , Fatores de TempoRESUMO
Heterotopic pregnancy (HP) is a very rare pathologic event. The incidence of ectopic pregnancy has increased as the consequence of assisted reproduction and the wider use of ovulation induction agents. The diagnosis of HP is frequently done not as earlier as it should be, and it has serious repercussions. The concept of screening, to find relevant rise factors and the correct diagnosis of HP should be pursued as early as possible. In this report we describe an unusual case of a female patient with therapeutic failure to clomiphene citrate. She was treated with two cycles of combined clomiphene citrate and prednisone, and ovulation induction assent. The after she developed HP. Overall, we conclude that the possible association observed could be an accident. The land of investigation is open, to fill cover. The holes on understanding that we still have.