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1.
Front Cell Dev Biol ; 9: 768783, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-35155442

RESUMO

The microbiota, the set of microorganisms associated with a particular environment or host, has acquired a prominent role in the study of many physiological and developmental processes. Among these, is the relationship between the microbiota and regenerative processes in various organisms. Here we introduce the concept of the microbiota and its involvement in regeneration-related cellular events. We then review the role of the microbiota in regenerative models that extend from the repair of tissue layers to the regeneration of complete organs or animals. We highlight the role of the microbiota in the digestive tract, since it accounts for a significant percentage of an animal microbiota, and at the same time provides an outstanding system to study microbiota effects on regeneration. Lastly, while this review serves to highlight echinoderms, primarily holothuroids, as models for regeneration studies, it also provides multiple examples of microbiota-related interactions in other processes in different organisms.

2.
Monoclon Antib Immunodiagn Immunother ; 37(2): 78-86, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29708866

RESUMO

Platelets play a vital role in hemostasis and inflammation. The membrane receptor TREM-like transcript-1 (TLT-1) is involved in platelet aggregation, bleeding, and inflammation, and it is localized in the α-granules of platelets. Upon platelet activation, TLT-1 is released from α-granules both in its transmembrane form and as a soluble fragment (sTLT-1). Higher levels of sTLT-1 have been detected in the plasma of patients with acute inflammation or sepsis, suggesting an important role for TLT-1 during inflammation. However, the roles of TLT-1 in hemostasis and inflammation are not well understood. We are developing the mouse model of TLT-1 to mechanistically test clinical associations of TLT-1 in health and disease. To facilitate our studies, monoclonal murine TLT-1 (mTLT-1) antibodies were produced by the immunization of a rabbit using the negatively charged region of the mTLT-1 extracellular domain 122PPVPGPREGEEAEDEK139. In the present study, we demonstrate that two selected clones, 4.6 and 4.8, are suitable for the detection of mTLT-1 by western blot, immunoprecipitation, immunofluorescent staining, flow cytometry and inhibit platelet aggregation in aggregometry assays. In addition, we found that the topical administration of clone 4.8 delayed the wound healing process in an experimental burn model. These results suggest that TLT-1 plays an important role in wound healing and because both clones specifically detect mTLT-1, they are suitable to further develop TLT-1 based models of inflammation and hemostasis in vivo.


Assuntos
Anticorpos Monoclonais/farmacologia , Queimaduras/imunologia , Agregação Plaquetária/efeitos dos fármacos , Receptores Imunológicos/imunologia , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Administração Cutânea , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/biossíntese , Anticorpos Monoclonais/isolamento & purificação , Plaquetas/química , Plaquetas/metabolismo , Western Blotting , Queimaduras/patologia , Células Clonais , Grânulos Citoplasmáticos/química , Grânulos Citoplasmáticos/metabolismo , Expressão Gênica , Imunização , Imunoprecipitação , Masculino , Camundongos , Peptídeos/administração & dosagem , Peptídeos/química , Peptídeos/imunologia , Coelhos , Receptores Imunológicos/química , Pele/imunologia , Pele/patologia , Cicatrização/imunologia
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