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The Characterization of Monoclonal Antibodies to Mouse TLT-1 Suggests That TLT-1 Plays a Role in Wound Healing.
Manfredi, Barbara; Morales-Ortíz, Jessica; Díaz-Díaz, Lymarie M; Hernandez-Matias, Liz; Barreto-Vázquez, Delmaliz; Menéndez-Pérez, Javier; Rodríguez-Cordero, J Alejandro; Villalobos-Santos, Juan C; Santiago-Rivera, Edgardo; Rivera-Dompenciel, Adriana; Lozada-Delgado, Eunice L; Kuchibhotla, Madhavi; Carrasquillo-Carrión, Kelvin; Roche-Lima, Abiel; Washington, A Valance.
Afiliação
  • Manfredi B; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Morales-Ortíz J; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Díaz-Díaz LM; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Hernandez-Matias L; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Barreto-Vázquez D; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Menéndez-Pérez J; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Rodríguez-Cordero JA; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Villalobos-Santos JC; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Santiago-Rivera E; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Rivera-Dompenciel A; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Lozada-Delgado EL; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Kuchibhotla M; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
  • Carrasquillo-Carrión K; 2 ARL Lab-Bioinformatics and Health Informatics, Recinto Central Medico, University of Puerto Rico , San Juan, Puerto Rico .
  • Roche-Lima A; 2 ARL Lab-Bioinformatics and Health Informatics, Recinto Central Medico, University of Puerto Rico , San Juan, Puerto Rico .
  • Washington AV; 1 Biology Department, University of Puerto Rico , San Juan, Puerto Rico .
Monoclon Antib Immunodiagn Immunother ; 37(2): 78-86, 2018 Apr.
Article em En | MEDLINE | ID: mdl-29708866
Platelets play a vital role in hemostasis and inflammation. The membrane receptor TREM-like transcript-1 (TLT-1) is involved in platelet aggregation, bleeding, and inflammation, and it is localized in the α-granules of platelets. Upon platelet activation, TLT-1 is released from α-granules both in its transmembrane form and as a soluble fragment (sTLT-1). Higher levels of sTLT-1 have been detected in the plasma of patients with acute inflammation or sepsis, suggesting an important role for TLT-1 during inflammation. However, the roles of TLT-1 in hemostasis and inflammation are not well understood. We are developing the mouse model of TLT-1 to mechanistically test clinical associations of TLT-1 in health and disease. To facilitate our studies, monoclonal murine TLT-1 (mTLT-1) antibodies were produced by the immunization of a rabbit using the negatively charged region of the mTLT-1 extracellular domain 122PPVPGPREGEEAEDEK139. In the present study, we demonstrate that two selected clones, 4.6 and 4.8, are suitable for the detection of mTLT-1 by western blot, immunoprecipitation, immunofluorescent staining, flow cytometry and inhibit platelet aggregation in aggregometry assays. In addition, we found that the topical administration of clone 4.8 delayed the wound healing process in an experimental burn model. These results suggest that TLT-1 plays an important role in wound healing and because both clones specifically detect mTLT-1, they are suitable to further develop TLT-1 based models of inflammation and hemostasis in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Cicatrização / Queimaduras / Receptores Imunológicos / Agregação Plaquetária / Anticorpos Monoclonais Limite: Animals Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Porto Rico País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Cicatrização / Queimaduras / Receptores Imunológicos / Agregação Plaquetária / Anticorpos Monoclonais Limite: Animals Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Porto Rico País de publicação: Estados Unidos