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Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus Metastrongylus, whose eggs are similar to other Strongylida through light microscopy; species-specific identification can be performed with molecular tools. We explored the species composition and the genetic diversity of Metastrongylus infecting pigs in close contact with humans in impoverished rural communities in the state of Piauí, in northeastern Brazil. Fecal samples (n = 78) were collected for parasitologic tests. Egg morphometry and molecular characterization, using the cytochrome c oxidase subunit 1 (cox1) gene, were performed. For strongyliform eggs, 62 of 78 (80%) pigs were positive and 6 of 99 (6%) eggs had dimensions compatible with Metastrongylus. Of the 37 samples submitted to PCR, 10 were identified as M. salmi. We found 3 M. salmi haplotypes, including 2 new and 1 described previously in Europe. Overall, M. salmi demonstrated lower intraspecific genetic diversity: diversity index (H) ± SD = 0.318 ± 0.164, n = 12, compared with published M. pudendotectus sequences (1.000 ± 0.272, n = 3). To our knowledge, M. salmi DNA sequences have not been published previously from pigs in South America.

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Helminths of the genus Oesophagostomum cause enteric diseases and affect domestic animals such as pigs. The aim of this study was to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans in the state of Piauí, Brazil. Eighty-seven fecal samples were collected for parasitological tests and molecular analysis. Through microscopy, the overall positivity rate for strongyliform eggs was 81.6% among the pigs studied. Forty-two strongyliform egg samples were subjected to PCR and six cox1 sequences (637 bp) were identified for the genus Oesophagostomum. The sequences were identified as Oesophagostomum dentatum, O. quadrispinulatum and O. columbianum. In the phylogenetic tree and haplotype network, 89 sequences were separated into seven clusters, which also included reference sequences from GenBank. Oesophagostomum dentatum and O. quadrispinulatum were seen to be closely related species and formed a monophyletic group related to O. aculeatum. Oesophagostomum columbianum showed similarity with sequences from parasites infecting small ruminants and the clade was positioned closer to O. bifurcum. High interspecific diversity was found and intraspecific diversity varied according to the species. This was the first study to characterize Oesophagostomum DNA sequences obtained from pigs in Brazil.

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Helminths of the genus Oesophagostomum cause enteric diseases and affect domestic animals such as pigs. The aim of this study was to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans in the state of Piauí, Brazil. Eighty-seven fecal samples were collected for parasitological tests and molecular analysis. Through microscopy, the overall positivity rate for strongyliform eggs was 81.6% among the pigs studied. Forty-two strongyliform egg samples were subjected to PCR and six cox1 sequences (637 bp) were identified for the genus Oesophagostomum. The sequences were identified as Oesophagostomum dentatum, O. quadrispinulatum and O. columbianum. In the phylogenetic tree and haplotype network, 89 sequences were separated into seven clusters, which also included reference sequences from GenBank. Oesophagostomum dentatum and O. quadrispinulatum were seen to be closely related species and formed a monophyletic group related to O. aculeatum. Oesophagostomum columbianum showed similarity with sequences from parasites infecting small ruminants and the clade was positioned closer to O. bifurcum. High interspecific diversity was found and intraspecific diversity varied according to the species. This was the first study to characterize Oesophagostomum DNA sequences obtained from pigs in Brazil.(AU)

Parasitos do gênero Oesophagostomum causam doenças entéricas e podem afetar a criação de animais, como os suínos. O objetivo deste estudo foi identificar as espécies e explorar a diversidade genética de Oesophagostomum spp. infectando suínos em contato próximo com humanos, no estado do Piauí, Brasil. Oitenta e sete amostras fecais foram coletadas para testes parasitológicos, análise morfométrica dos ovos e análises moleculares. A taxa geral de positividade para ovos estrongiliformes foi de 81,6%. Quarenta e duas amostras de ovos estrongiliformes foram submetidas à PCR e seis sequências cox1 (637 bp) foram identificadas para o gênero Oesophagostomum. As sequências foram identificadas como Oesophagostomum dentatum, O. quadrispinulatum e O. columbianum. Na árvore filogenética e na rede haplotípica, 89 sequências foram separadas em sete clusters, incluindo sequências de referência do GenBank. Oesophagostomum dentatum e O. quadrispinulatum são espécies estreitamente relacionadas e formaram um grupo monofilético com O. aculeatum. Oesophagostomum columbianum apresentou semelhança com sequências de parasitas obtidos de pequenos ruminantes e o clado foi posicionado mais próximo de O. bifurcum. Alta diversidade interespecífica foi encontrada e a diversidade intraespecífica variou de acordo com as espécies. Esse foi o primeiro estudo a caracterizar sequências de DNA de Oesophagostomum isoladas de suínos no Brasil.(AU)

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BACKGROUND: This study assessed the interactions between income, nutritional status and intestinal parasitism in children in Brazil. METHODS: A cross-sectional study (n = 421 children aged 1 to 14 years living in the states of Piauí (rural communities in the city of Teresina) and Rio de Janeiro (rural and periurban communities in the city of Cachoeiras de Macacu) was performed in order to obtain income and anthropometric data, as well as fecal samples for parasitological analyses through the Ritchie technique. RESULTS: Children infected with Ascaris lumbricoides had significantly lower means of height-for-age z scores (- 1.36 ± 0.75 vs. - 0.11 ± 1.02; p < 0.001), weight-for-age z scores (- 1.23 ± 0.74 vs. 0.09 ± 1.15; p = 0.001), and weight-for-height z scores (- 0.68 ± 0.44 vs. 0.23 ± 1.25; p = 0.006) when compared with uninfected children. Infection with hookworm was also associated with lower means of height-for-age z scores (- 1.08 ± 1.17 vs. - 0.12 ± 1.02; p = 0.015) and weight-for-age z scores (- 1.03 ± 1.13 vs. 0.08 ± 1.15; p = 0.012). Children infected with Entamoeba coli presented significantly lower means of height-for-age z scores (- 0.54 ± 1.02 vs. - 0.09 ± 1.02; p = 0.005) and weight-for-age z scores (- 0.44 ± 1.15 vs. 0.12 ± 1.15; p = 0.002). The multivariate multiple linear regression analysis showed that height-for-age z scores are independently influenced by monthly per capita family income (ß = 0.145; p = 0.003), female gender (ß = 0.117; p = 0.015), and infections with A. lumbricoides (ß = - 0.141; p = 0.006) and Entamoeba coli (ß = - 0.100; p = 0.043). Weight-for-age z scores are influenced by monthly per capita family income (ß = 0.175; p < 0.001), female gender (ß = 0.123; p = 0.010), and infections with A. lumbricoides (ß = - 0.127; p = 0.012), and Entamoeba coli (ß = - 0.101; p = 0.039). Monthly per capita family income (ß = 0.102; p = 0.039) and female gender (ß = 0.134; p = 0.007) positively influences mid upper arm circumpherence. CONCLUSIONS: Intestinal parasitism and low family income negatively influence the physical development of children in low-income communities in different Brazilian regions.

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BACKGROUND: Entamoeba species harbored by humans have different degrees of pathogenicity. The present study explores the intra- and interspecific diversity, phylogenetic relationships, prevalence and distribution of tetra- and octonucleated cyst-producing Entamoeba in different Brazilian regions. METHODS: Cross-sectional studies were performed to collect fecal samples (n = 1728) and sociodemographic data in communities located in four Brazilian biomes: Atlantic Forest, Caatinga, Cerrado, and Amazon. Fecal samples were subjected to molecular analysis by partial small subunit ribosomal DNA sequencing (SSU rDNA) and phylogenetic analysis. RESULTS: Light microscopy analysis revealed that tetranucleated cysts were found in all the studied biomes. The highest positivity rates were observed in the age group 6-10 years (23.21%). For octonucleated cysts, positivity rates ranged from 1 to 55.1%. Sixty SSU rDNA Entamoeba sequences were obtained, and four different species were identified: the octonucleated E. coli, and the tetranucleated E. histolytica, E. dispar, and E. hartmanni. Novel haplotypes (n = 32) were characterized; however, new ribosomal lineages were not identified. The Entamoeba coli ST1 subtype predominated in Atlantic Forest and Caatinga, and the ST2 subtype was predominant in the Amazon biome. E. histolytica was detected only in the Amazon biome. In phylogenetic trees, sequences were grouped in two groups, the first containing uni- and tetranucleated and the second containing uni- and octonucleated cyst-producing Entamoeba species. Molecular diversity indexes revealed a high interspecific diversity for tetra- and octonucleated Entamoeba spp. (H ± SD = 0.9625 ± 0.0126). The intraspecific diversity varied according to species or subtype: E. dispar and E. histolytica showed lower diversity than E. coli subtypes ST1 and ST2 and E. hartmanni. CONCLUSIONS: Tetra- and octonucleated cyst-producing Entamoeba are endemic in the studied communities; E. histolytica was found in a low proportion and only in the Amazon biome. With regard to E. coli, subtype ST2 was predominant in the Amazon biome. The molecular epidemiology of Entamoeba spp. is a field to be further explored and provides information with important implications for public health.

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In this study, we assessed the genetic diversity of Ascaris lumbricoides / Ascaris suum circulating in humans and pigs, exploring potential zoonotic cycles in endemic areas in Brazil. We carried out cross-sectional surveys in four municipalities: Santa Isabel do Rio Negro (SIRN-AM) (n = 328); Nossa Senhora de Nazaré (NSN-PI) and Teresina (TER-PI) (n = 605 and n = 297, respectively); and Cachoeiras de Macacu (CAM-RJ) (n = 543). We also studied 61 fecal samples/adult worms obtained from pigs (n = 53 in NSN-PI and n = 8 in TER-PI). A ~450 bp fragment of the Ascaris cytochrome c oxidase subunit 1 (cox1) and ~400 bp of the NADH dehydrogenase subunit 1 (nad1) were amplified and sequenced. Maximum-likelihood (ML) tree and Median-joining (MJ) haplotype network analyses were performed. We also performed scanning electron micrographs of adult specimens. Positivity rates were 93/328 (28.4%) in SIRN-AM, 6/297 (2.0%) in TER-PI, 0/605 (0%) in NSN-PI, and 6/543 (1.1%) in CAM-RJ. In NSN-PI it reached 11/53 (20.7%) in pigs. The MJ network based on cox1 locus (383 bp) revealed three main clusters, one centered around haplotypes H01/H28/H32 and the other around H07/H11. The cox1 haplotypes had a heterogeneous distribution, showing no pattern by geographic region, and high haplotype diversity. The ML trees based on cox1 and nad1 loci showed a similar topology with each other, and with the haplotype networks. Three distinct clusters were observed. Sequences of cox1 and nad1 from humans and animals were distributed throughout the tree and it was not possible to differentiate specimens of human and swine origin. Ascaris populations obtained from humans and swine in different Brazilian regions are not discriminable through the genetic markers used, which indicates the potential for zoonotic transmission and the need for better control of these infections in swine herds, mainly when created in a peridomestic environment.

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INTRODUCTION: The present study aimed to determine the prevalence and factors associated with Giardia intestinalis infection, verifying its impact on the nutritional status of children in northeastern Brazil. METHODOLOGY: A cross-sectional study was conducted to obtain parasitological, sociodemographic, and anthropometric data in two municipalities in the states of Piauí and Ceará, northeastern Brazil. RESULTS: Prevalence of giardiasis was 55/511 (10.8%). G. intestinalis was more frequent in people living in poverty (30/209 [14.4%], p = 0.041), performing open evacuation (26/173 [15%], p = 0.034), and drinking rainwater stored in cisterns (9/56 [16.1%], p = 0.005). The proportion of stunting and being underweight in children infected with G. intestinalis was significantly higher than that in uninfected children (5/23 [21.7%] vs. 10/179 [5.6%], p = 0.017, OR = 4.69, 95% confidence interval [CI] = 1.44-15.25 and 5/23 [21.7%] vs. 13/179 [7.3%], p = 0.038, OR = 3.54, 95% CI = 1.13-11.09, respectively). Infection with G. intestinalis remained significantly associated with stunting and being underweight after adjustment for poverty, municipality, sex, and age in a logistic regression multivariate model. CONCLUSIONS: In rural areas in northeastern Brazil, giardiasis has acquired great public health importance in the soil-transmitted helminths control era, impacting the nutritional status of children and requiring new approaches to diagnosis and treatment and translational research that could generate applicable solutions at the community level.

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BACKGROUND: Current control policies for intestinal parasitosis focuses on soil-transmitted helminths, being ineffective against Giardia intestinalis, a highly prevalent protozoon that impacts children's nutritional status in developing countries. The objective of this study was to explore spatial and molecular epidemiology of Giardia intestinalis in children of Amerindian descent in the Brazilian Amazon. METHODOLOGY/PRINCIPAL FINDINGS: A cross sectional survey was performed in the Brazilian Amazon with 433 children aged 1 to 14 years. Fecal samples were processed through parasitological techniques and molecular characterization. Prevalence of G. intestinalis infection was 16.9% (73/433), reaching 22.2% (35/158) among children aged 2-5 years, and a wide distribution throughout the city with some hot spots. Positivity-rate was similar among children living in distinct socioeconomic strata (48/280 [17.1%] and 19/116 [16.4%] below and above the poverty line, respectively). Sequencing of the ß-giardin gene revealed 52.2% (n = 12) of assemblage A and 47.8% (n = 11) of assemblage B with high haplotype diversity for the latter. The isolates clustered into two well-supported G. intestinalis clades. A total of 38 haplotypes were obtained, with the following subassemblages distribution: 5.3% (n = 2) AII, 26.3% (n = 10) AIII, 7.9% (n = 3) BIII, and 60.5% (n = 23) new B genotypes not previously described. CONCLUSIONS/SIGNIFICANCE: Giardia intestinalis infection presents a high prevalence rate among Amerindian descended children living in Santa Isabel do Rio Negro/Amazon. The wide distribution observed in a small city suggests the presence of multiple sources of infection, which could be related to environmental contamination with feces, possibly of human and animal origin, highlighting the need of improving sanitation, safe water supply and access to diagnosis and adequate treatment of infections.

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    A contaminação ambiental por fezes de cães e gatos tem sido uma preocupação constante em áreas urbanas, devido principalmente à possibilidade de transmissão de zoonoses. É comum o encontro de massas fecais nas calçadas de alguns bairros do município de Niterói. O objetivo deste trabalho foi determinar a prevalência de parasitos em massas fecais recolhidas em oito bairros do município de Niterói. Duzentas e quarenta amostras de massas fecais foram recolhidas em vias públicas e processadas no Laboratório de Doenças Parasitárias da Faculdade de Veterinária da UFF, utilizando as técnicas de sedimentação simples e centrifugo-flutuação em solução de sulfato de zinco.  A prevalência das massas fecais positivas para a presença de formas parasitárias foi de 18,3%. Destas 22,7% foram encontradas em Charitas, 20,7% em Jurujuba, 13,6% no Vital Brazil, 13,6% no Ingá, 11,4% em São Francisco, 9,1% em Santa Rosa, 4,5% em Icaraí e 4,5% em Piratininga. Das formas parasitárias encontradas 76% foram ovos de ancilostomatídeos, 9% cápsulas ovígeras de Dipylidium sp., 9% ovos de Trichuris sp., 4% ovos de Toxocara sp. e 2% de formas vegetativas de Entamoeba sp. Estes dados são úteis como indicadores de risco para a saúde animal e humana, bem como para campanhas de conscientização com enfoque em Saúde Pública. 

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A contaminação ambiental por fezes de cães e gatos tem sido uma preocupação constante em áreas urbanas, devido principalmente à possibilidade de transmissão de zoonoses. É comum o encontro de massas fecais nas calçadas de alguns bairros do município de Niterói. O objetivo deste trabalho foi determinar a prevalência de parasitos em massas fecais recolhidas em oito bairros do município de Niterói. Duzentas e quarenta amostras de massas fecais foram recolhidas em vias públicas e processadas no Laboratório de Doenças Parasitárias da Faculdade de Veterinária da UFF, utilizando as técnicas de sedimentação simples e centrifugo-flutuação em solução de sulfato de zinco.  A prevalência das massas fecais positivas para a presença de formas parasitárias foi de 18,3%. Destas 22,7% foram encontradas em Charitas, 20,7% em Jurujuba, 13,6% no Vital Brazil, 13,6% no Ingá, 11,4% em São Francisco, 9,1% em Santa Rosa, 4,5% em Icaraí e 4,5% em Piratininga. Das formas parasitárias encontradas 76% foram ovos de ancilostomatídeos, 9% cápsulas ovígeras de Dipylidium sp., 9% ovos de Trichuris sp., 4% ovos de Toxocara sp. e 2% de formas vegetativas de Entamoeba sp. Estes dados são úteis como indicadores de risco para a saúde animal e humana, bem como para campanhas de conscientização com enfoque em Saúde Pública.