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Molecular detection of Metastrongylus salmi eggs from pigs in low-resource communities in the state of Piauí, northeastern Brazil.
Bacelar, Polyanna A A; Jaeger, Lauren H; Calegar, Deiviane A; Santos, Jéssica P; Coronato-Nunes, Beatriz; Reis, Elis R C; Bóia, Márcio N; Monteiro, Kerla J L; Carvalho-Costa, Filipe A.
Afiliação
  • Bacelar PAA; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
  • Jaeger LH; Oswaldo Cruz Foundation Regional Office-Piauí, Teresina, Piauí, Brazil.
  • Calegar DA; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Juiz de Fora Federal University, Juiz de Fora, Brazil.
  • Santos JP; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
  • Coronato-Nunes B; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
  • Reis ERC; Oswaldo Cruz Foundation Regional Office-Piauí, Teresina, Piauí, Brazil.
  • Bóia MN; Arthur Sá Earp Neto University Center/Petropolis Faculty of Medicine (UNIFASE/FMP), Petrópolis, Rio de Janeiro, Brazil.
  • Monteiro KJL; Municipal Health Department of Nossa Senhora de Nazaré, Nossa Senhora de Nazaré, Piauí, Brazil.
  • Carvalho-Costa FA; Laboratory of Biology and Parasitology of Wild Reservoirs Mammals, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
J Vet Diagn Invest ; 34(4): 689-692, 2022 Jul.
Article em En | MEDLINE | ID: mdl-35610940
Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus Metastrongylus, whose eggs are similar to other Strongylida through light microscopy; species-specific identification can be performed with molecular tools. We explored the species composition and the genetic diversity of Metastrongylus infecting pigs in close contact with humans in impoverished rural communities in the state of Piauí, in northeastern Brazil. Fecal samples (n = 78) were collected for parasitologic tests. Egg morphometry and molecular characterization, using the cytochrome c oxidase subunit 1 (cox1) gene, were performed. For strongyliform eggs, 62 of 78 (80%) pigs were positive and 6 of 99 (6%) eggs had dimensions compatible with Metastrongylus. Of the 37 samples submitted to PCR, 10 were identified as M. salmi. We found 3 M. salmi haplotypes, including 2 new and 1 described previously in Europe. Overall, M. salmi demonstrated lower intraspecific genetic diversity: diversity index (H) ± SD = 0.318 ± 0.164, n = 12, compared with published M. pudendotectus sequences (1.000 ± 0.272, n = 3). To our knowledge, M. salmi DNA sequences have not been published previously from pigs in South America.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Suínos / Infecções por Strongylida / Metastrongyloidea Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Vet Diagn Invest Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Suínos / Infecções por Strongylida / Metastrongyloidea Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Vet Diagn Invest Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos