RESUMO
The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were obtained for light microscopy (LM), transmission electron microscopy procedures, and to detect apoptosis and p53 antigen by immunohistochemical methods. Blood was collected to quantify testosterone and plasmatic cholinesterase activity. From 8.3 days, Sertoli cell vacuolization, karyolisis of pachytene spermatocytes and Leydig cells and a decreased in average of the diameter of seminiferous tubules was observed. No damage to inter-Sertoli cells junctions was detected. Percentage of seminiferous tubules showing germ cells apoptosis was increased from 8.3 days, plasmatic acetylcholinesterase activity was reduced in the group treated only 24 hours after administration of MP. Serum testosterone levels were low in treated animals at 16. 6 and 33.2 days. p53 was mostly expressed in pachytene spermatocytes from 8d. The findings of this study indicate that MP alters the testicular function affecting the DNA and interfering with spermatogenesis as well as steroidogenesis.
La restricción de los mecanismos de proliferación celular en epitelio seminífero murino, en términos de inducción de muerte celular programada hasta hace poco no había sido completamente analizada.El objetivo de este trabajo fue evaluar el efecto de malathion (MP) sobre la morfología y la función testicular del ratón.Ratones macho albinos de la cepa NMRI-IVIC, con pesos entre 30-40 g fueron utilizados, se dividieron en grupos control y experimental. Los grupos experimentales fueron inyectados por vía intraperitoneal con una dosis única deMP:241mg/kg de peso (1/12 DL50) resuspendido en 0,9% de solución salina.Los animales fueron sacrificados en el día 8,3, 16,6 y 33,2 después de la inyección (primer, segundo y tercer ciclos de la espermatogénesis).Se obtuvieron muestras de testículo para estudio en microscopía de luz (ML), microscopía electrónica de transmisión, para la detección de apoptosis y el antígeno p53 (proliferación celular), por métodos inmunohistoquímicos.Se recogió sangre para cuantificar la testosterona y la actividad plasmática de colinesterasa.Desde el día 8,3 día se observó vacuolización de células de Sertoli, cariolisis de espermatocitos en paquiteno y células de Leydig, y una disminución en el promedio del diámetro de los túbulos seminíferos. No se detectó daño en las uniones entre células de Sertoli. El porcentaje de túbulos seminíferos que mostraban células germinales en apoptosis se incrementó a los 8,3 días, laactividad de la acetilcolinesterasa plasmática se redujo en el grupo tratado sólo 24 horas después de la administración de MP.Los niveles séricos de testosterona disminuyeron en los animales tratados a los 16,6 y 33,2 días.P53 se expresó sobre todo en los espermatocitos en paquiteno desde los 8,3 días.Los resultados de este estudio indican que MP altera la función testicular, afecta al ADN e interfiere con la espermatogénesis, así como con la esteroidogénesis.
Assuntos
Animais , Masculino , Camundongos , Espermatogênese/efeitos dos fármacos , Espermatozoides/citologia , Proliferação de Células/efeitos dos fármacos , Malation/toxicidade , Espermatozoides/efeitos dos fármacos , ApoptoseRESUMO
La Amebiasis Extraintestinal es una presentación de la forma intestinal que implica complicaciones severas. El objetivo de este estudio fue determinar la prevalencia de la amebiasis extraintetinal en el estado Bolívar. Se realizó un estudio descriptivo-retrospectivo de los casos de amebiasis extraintestinal del estado durante el período 1996-2001. La única forma de amebiasis extraintestinal encontrada fue el absceso hepático amebiano (6 casos), con una prevalencia de 7,1/1.000 pacientes hospitalizados con amebiasis. El sexo que predominó fue el masculino (4:2). La edad osciló entre 36 y 73 años. Las manifestaciones clínicas presentadas fueron; fiebre, dolor abdominal, hiporexia y hepatomegalia. Se evidenció anemia (n=6), neutrofilia (n=4) y elevación de las aminotransferasas. El diagnóstico se realizó mediante e ecosografía, tomografía y demostración de anticuerpos específicos para E.histolytica. Para el tratamiento se utilizó metronidazol en todos los casos, asociándose con otro antibiótico en 4 pacientes. La amebiasis extraintestinal es poco frecuente en los principales hospitales del estado Bolívar y el absceso hepático amebiano constituye la única forma de presentación.
Assuntos
Humanos , Masculino , Adulto , Feminino , Pessoa de Meia-Idade , Disenteria Amebiana , Metronidazol , Microbiologia , VenezuelaRESUMO
We assessed the usefulness of an agar diffusion method, NeoSensitabs, to determine in vitro sensitivity of 52 isolates of dematiaceous filamentous fungi against ten antifungal agents: amphotericin B, 5-fluorocytosine, ketoconazole, fluconazole, itraconazole, terbinafine, bifonazole, miconazole, clotrimazole, and griseofulvin. For the preparation of the inoculum, a spectrophotometric method including both Shadomy and Casitone agar (CAS) culture media was used. Dematiaceous filamentous fungi were sensitive to itraconazole, terbinafine and bifonazole. Ketoconazole (90.4%), miconazole (71%), and clotrimazole (46%) showed a variable susceptibility pattern. Most species were resistant to griseofulvin and fluconazole (96%). All isolates were resistant to 5-fluorocytosine. Sixty-three percent of strains were susceptible to amphotericin B and 28.8% resistant. Inhibition zones in the antifungal susceptibility testing did not vary according to culture medium, although fungal growth was better in CAS. Variations in antifungal sensitivity in Exophiala spinifera and Fonsecaea pedrosoi spp. would justify an in vitro susceptibility study when indicating antifungal therapy. These results show that NeoSensitabs agar diffusion method is simple, rapid, and low-cost and can be available to many clinical laboratories for the study of in vitro sensitivity of dematiceous moulds.
RESUMO
Systemic infections caused by opportunistic fungi have shown an increased frequency in the past 10 years, particularly in immunocompromised patients. Hansenula anomala is an ascosporogenous yeast of the Ascomycetes class found in the skin, throat, and digestive tract transient normal flora. This study was conducted to compare the pathogenicity of H. anomala and Candida albicans in a model of immunocompromised mice. Thirty-eight Swiss mice were divided into two groups as follows: 30 animals received an intraperitoneal (i.p.) injection of cyclophosphamide (200 mg/kg) four days before the induction of infection with H. anomala (1 x 10(6) yeasts/mL), and 8 animals received 100 mg/kg of cyclophosphamide at 3-day intervals during 3 weeks before inoculation of 1 x 10(7) yeasts/mL. All animals were treated with amoxicillin/clavulanic acid (40 mg/kg) four days before induction of infection. A group of mice inoculated with C. albicans (ATCC 64548) served as control. Tissue samples from the lung, spleen, liver, and kidney for histological and mycologic studies were obtained at necropsy. In each animal, the number of viable yeasts per gram of kidney was determined. The organs most frequently infected by H. anomala were the kidneys and the liver (20%), and the lung (10%). However, in conditions of sustained immunosuppression, H. anomala was found in 65.5% of the organs examined. It is concluded that in an experimental model of immunocompromised mice, the pathogenicity of H. anomala was low.
Assuntos
Micoses/microbiologia , Pichia/patogenicidade , Amoxicilina/uso terapêutico , Animais , Contagem de Colônia Microbiana , Ciclofosfamida , Feminino , Humanos , Hospedeiro Imunocomprometido , Imunossupressores , Rim/microbiologia , Fígado/microbiologia , Pulmão/microbiologia , Camundongos , Micoses/tratamento farmacológico , Micoses/imunologia , Neutropenia/induzido quimicamente , Penicilinas/uso terapêutico , VirulênciaRESUMO
The aim of this study was to adapt a spectrophotometric method for preparing the inocula of dematiaceous fungi used for in vitro susceptibility tests. Fifty-two isolates of 17 different species of dematiaceous fungi were used for this purpose. Homogeneous suspensions of conidia and hyphae of these isolates were obtained and adjusted for reading at 530 and 550 nm at 40% and 50% of transmittance. The suspensions were standardised to 1-5 x 10 e6 CFU/ml. Quality controls of the inocula were done by quantitative cultures on agar-Sabouraud plates. The inocula obtained by spectrophotometry showed little variability within all the isolates. This method can be useful for in vitro antifungal evaluation of dematiaceous fungi.