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[In vitro susceptibility of dematiaceous fungi to ten antifungal drugs using an agar diffusion test]. / Sensibilidad de hongos miceliares dematiaceos a diez antifúngicos empleando un método de difusión en agar.
Cermeño-Vivas, J R; Torresrodríguez, J M.
Afiliação
  • Cermeño-Vivas JR; Departamento de Parasitología y Microbiología, Escuela de Medicina, Universidad de Oriente, Ciudad Bolívar, Venezuela.
Rev Iberoam Micol ; 18(3): 113-7, 2001 Sep.
Article em Es | MEDLINE | ID: mdl-15487919
We assessed the usefulness of an agar diffusion method, NeoSensitabs, to determine in vitro sensitivity of 52 isolates of dematiaceous filamentous fungi against ten antifungal agents: amphotericin B, 5-fluorocytosine, ketoconazole, fluconazole, itraconazole, terbinafine, bifonazole, miconazole, clotrimazole, and griseofulvin. For the preparation of the inoculum, a spectrophotometric method including both Shadomy and Casitone agar (CAS) culture media was used. Dematiaceous filamentous fungi were sensitive to itraconazole, terbinafine and bifonazole. Ketoconazole (90.4%), miconazole (71%), and clotrimazole (46%) showed a variable susceptibility pattern. Most species were resistant to griseofulvin and fluconazole (96%). All isolates were resistant to 5-fluorocytosine. Sixty-three percent of strains were susceptible to amphotericin B and 28.8% resistant. Inhibition zones in the antifungal susceptibility testing did not vary according to culture medium, although fungal growth was better in CAS. Variations in antifungal sensitivity in Exophiala spinifera and Fonsecaea pedrosoi spp. would justify an in vitro susceptibility study when indicating antifungal therapy. These results show that NeoSensitabs agar diffusion method is simple, rapid, and low-cost and can be available to many clinical laboratories for the study of in vitro sensitivity of dematiceous moulds.
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Coleções: 01-internacional Base de dados: MEDLINE Idioma: Es Revista: Rev Iberoam Micol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Espanha
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Idioma: Es Revista: Rev Iberoam Micol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Espanha