RESUMO
INTRODUCTION: Genetic studies have shown associations of several single nucleotide polymorphisms (SNP) with different rates of progression and variation in susceptibility to HIV infection. This study aimed to estimate the frequency of ccr5Δ32, IL-6-174G/C, IFN-γ+874T/A and IL-10-1082A/G polymorphisms in Cuban HIV-infected patients and a group of sero-discordant couples to assess their influence on risk and disease progression. METHODS: A cross-sectional study was carried out on 120 subjects registered at the Institute of Tropical Medicine «Pedro Kour¼ (IPK) and the Ameijeiras Hospital from June 2018 until December 2019. The amplification of fragments of the ccr5, IL-6, IFN-γ and IL-10 genes was performed by polymerase chain reaction followed by identification of polymorphisms using the restriction fragment length polymorphism analysis for IL-6 with the restriction enzymes Nla III. Amplification Refractory Mutation System was used for IFN-γ and IL-10 genes. RESULTS: The allelic and genotypic distributions of the genes ccr5, IL-6, IFN-γ and IL-10 did not differ significantly between the two groups. Cell counts and plasma viral load values did not differ significantly between genotypes of the ccr5, IL-6, IFN-γ and IL-10 genes. Only the IL-6 GC genotype was associated with higher viral load values. The combination of alleles of the four considered SNPs showed a highly significant increase in the risk of HIV infection for one of them, but with a very low frequency (<1%). CONCLUSION: This study contributes to evaluating the frequency of these polymorphisms and their influence on biomarkers of the progression of HIV infection in the Cuban HIV-population.
Assuntos
Síndrome da Imunodeficiência Adquirida , Infecções por HIV , Humanos , Infecções por HIV/genética , Síndrome da Imunodeficiência Adquirida/genética , Interleucina-6/genética , Interleucina-10/genética , Estudos Transversais , Frequência do Gene , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Receptores CCR5/genéticaRESUMO
Pneumocystis pneumonia (PcP) is a serious fungal infection among immunocompromised patients. In developed countries, the epidemiology and clinical spectrum of PcP have been clearly defined and well documented. However, in most developing countries, relatively little is known about the prevalence of pneumocystosis. Several articles covering African, Asian and American countries were reviewed in the present study. PcP was identified as a frequent opportunistic infection in AIDS patients from different geographic regions. A trend to an increasing rate of PcP was apparent in developing countries from 2002 to 2010.
Assuntos
Países em Desenvolvimento/estatística & dados numéricos , Hospedeiro Imunocomprometido , Pneumocystis carinii , Pneumonia por Pneumocystis/epidemiologia , África/epidemiologia , América/epidemiologia , Ásia/epidemiologia , PrevalênciaRESUMO
Os autores atualizam os conhecimentos relativos a glomerulonefrite difusa aguda pos-estreptococica, discutindo seus diferentes aspectos e ressaltando a moderna conduta terapeutica, que preve o tratamento ambulatorial dos pacientes
Assuntos
Glomerulonefrite , Infecções Estreptocócicas , Assistência AmbulatorialRESUMO
Os autores abordam o conceito de asma, sua fisiopatologia - discutindo, em particular, os aspectos imunologicos - a classificacao atual da doenca e os esquemas de tratamento propostos para a crise aguda, o estado de mal asmatico e a imunoterapia. Finalizando, analisam levantamentos estatisticos realizados em Santo Andre, no Estado de Sao Paulo, evidenciando a influencia do fator ambiental no desencadeamento das crises de asma