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Polymorphisms of CCR5, IL-6, IFN-γ and IL-10 genes in Cuban HIV/AIDS patients.
Hernández Requejo, D; de Armas, Y; Iglesias, E; Díaz, H M; Gravier, R; Godínez López, M C; Fonte, L; Plascencia-Cruz, M; Pacheco-Quijano, K; Palomares, J; Pérez-Gómez, H R; Plascencia-Hernández, A; Calderón, E J.
Afiliação
  • Hernández Requejo D; Departamento de Laboratorio Clínico, Centro Hospitalario, Instituto de Medicina Tropical «Pedro Kourí¼, La Habana, Cuba.
  • de Armas Y; Departamento de Patología, Centro Hospitalario, Instituto de Medicina Tropical «Pedro Kourí¼, La Habana, Cuba; Departamento de Diagnóstico Microbiológico Clínico, Centro Hospitalario, Instituto de Medicina Tropical «Pedro Kourí¼, La Habana, Cuba.
  • Iglesias E; Centro de Ingeniería Genética y Biotecnología, La Habana, Cuba.
  • Díaz HM; Hospital Clínico Quirúrgico «Hermanos Ameijeiras¼, La Habana, Cuba.
  • Gravier R; Departamento de Farmacología, Instituto de Medicina Tropical «Pedro Kourí¼, La Habana, Cuba.
  • Godínez López MC; Departamento de Laboratorio Clínico, Centro Hospitalario, Instituto de Medicina Tropical «Pedro Kourí¼, La Habana, Cuba.
  • Fonte L; Departamento de Parasitología, Instituto de Medicina Tropical «Pedro Kourí¼, La Habana, Cuba.
  • Plascencia-Cruz M; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.
  • Pacheco-Quijano K; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.
  • Palomares J; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.
  • Pérez-Gómez HR; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico.
  • Plascencia-Hernández A; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico. Electronic address: aplascenciah@yahoo.com.mx.
  • Calderón EJ; Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, Sevilla, Spain; Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain; Departamento de Medicina, Facultad
Rev Clin Esp (Barc) ; 224(2): 96-104, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38253256
ABSTRACT

INTRODUCTION:

Genetic studies have shown associations of several single nucleotide polymorphisms (SNP) with different rates of progression and variation in susceptibility to HIV infection. This study aimed to estimate the frequency of ccr5Δ32, IL-6-174G/C, IFN-γ+874T/A and IL-10-1082A/G polymorphisms in Cuban HIV-infected patients and a group of sero-discordant couples to assess their influence on risk and disease progression.

METHODS:

A cross-sectional study was carried out on 120 subjects registered at the Institute of Tropical Medicine «Pedro Kour¼ (IPK) and the Ameijeiras Hospital from June 2018 until December 2019. The amplification of fragments of the ccr5, IL-6, IFN-γ and IL-10 genes was performed by polymerase chain reaction followed by identification of polymorphisms using the restriction fragment length polymorphism analysis for IL-6 with the restriction enzymes Nla III. Amplification Refractory Mutation System was used for IFN-γ and IL-10 genes.

RESULTS:

The allelic and genotypic distributions of the genes ccr5, IL-6, IFN-γ and IL-10 did not differ significantly between the two groups. Cell counts and plasma viral load values did not differ significantly between genotypes of the ccr5, IL-6, IFN-γ and IL-10 genes. Only the IL-6 GC genotype was associated with higher viral load values. The combination of alleles of the four considered SNPs showed a highly significant increase in the risk of HIV infection for one of them, but with a very low frequency (<1%).

CONCLUSION:

This study contributes to evaluating the frequency of these polymorphisms and their influence on biomarkers of the progression of HIV infection in the Cuban HIV-population.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por HIV / Síndrome da Imunodeficiência Adquirida Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Cuba Idioma: En Revista: Rev Clin Esp (Barc) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por HIV / Síndrome da Imunodeficiência Adquirida Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Cuba Idioma: En Revista: Rev Clin Esp (Barc) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha