RESUMO
Melipona quadrifasciata anthidioides and Scaptotrigona depilis are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions and evaluate the antimicrobial activity of propolis produced by M. q. anthidioides and S. depilis. The ethanolic extracts of propolis of M. q. anthidioides (EEP-M) and S. depilis (EEP-S) were prepared, and their chemical constituents were characterized by HPLC-ESI-MS. The antimicrobial activity was evaluated against bacteria and fungi, isolated from reference strains and hospital origin resistant to the action of antibiotics. From EEP-M, phenolic compounds were annotated, including gallic acid, ellagic acid, and flavonoids, as well as diterpenes and triterpenes. EEP-S showed mainly triterpene in its chemical composition. Both extracts inhibited the growth of medically relevant bacteria and fungi, including hospital-acquired and antimicrobial-resistant. In general, EEP-S showed better antimicrobial activity compared to EEP-M. The MIC of EEP-S against vancomycin-resistant Enterococcus faecalis was 3.50 mg/mL, while the MIC of EEP-M was 5.33 ± 0.16 mg/mL. In conclusion, this study shows that propolis produced by M. q. anthidioides and S. depilis has the potential to be used for the prevention or treatment of microbial infections.
RESUMO
Natural products are important sources of biomolecules possessing antitumor activity and can be used as anticancer drug prototypes. The rich biodiversity of tropical and subtropical regions of the world provides considerable bioprospecting potential, including the potential of propolis produced by stingless bee species. Investigations of the potential of these products are extremely important, not only for providing a scientific basis for their use as adjuvants for existing drug therapies but also as a source of new and potent anticancer drugs. In this context, this article organizes the main studies describing the anticancer potential of propolis from different species of stingless bees with an emphasis on the chemical compounds, mechanisms of action, and cell death profiles. These mechanisms include apoptotic events; modulation of BAX, BAD, BCL2-L1 (BCL-2 like 1), and BCL-2; depolarization of the mitochondrial membrane; increased caspase-3 activity; poly (ADP-ribose) polymerase (PARP) cleavage; and cell death induction by necroptosis via receptor interacting protein kinase 1 (RIPK1) activation. Additionally, the correlation between compounds with antioxidant and anti-inflammatory potential is demonstrated that help in the prevention of cancer development. In summary, we highlight the important antitumor potential of propolis from stingless bees, but further preclinical and clinical trials are needed to explore the selectivity, efficacy, and safety of propolis.
Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Própole/farmacologia , Animais , Antineoplásicos/química , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Humanos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/fisiologia , Própole/química , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacosRESUMO
Propolis is a complex bioactive mixture produced by bees, known to have different biological activities, especially in countries where there is a rich biodiversity of plant species. The objective of this study was to determine the chemical composition and evaluate the antioxidant and cytotoxic properties of Brazilian propolis from the species Plebeia droryana and Apis mellifera found in Mato Grosso do Sul, Brazil. In the ethanolic extracts of P. droryana propolis (ExEP-P) and A. mellifera (ExEP-A) acids, phenolic compounds, terpenes and tocopherol were identified as major compounds. Both extracts presented antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, the maximum activities being 500 µg/mL (ExEP-P) and 300 µg/mL (ExEP-A). However, only ExEP-A was able to inhibit lipid peroxidation induced by the oxidizing agent 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), which inhibited oxidative hemolysis and reduced the levels of malondialdehyde (MDA) in human erythrocytes for 4 h of incubation. The extracts also reduced the cell viability of the K562 erythroleukemia tumour line, with a predominance of necrotic death. Thus, it is concluded that the propolis produced by P. droryana and A. mellifera contain important compounds capable of minimizing the action of oxidizing substances in the organism and reducing the viability of erythroleukemia cells.
Assuntos
Antioxidantes/farmacologia , Himenópteros/química , Própole/farmacologia , Animais , Antioxidantes/química , Antioxidantes/toxicidade , Brasil , Caspase 3/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Fracionamento Químico , Cromatografia Líquida de Alta Pressão , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Radicais Livres/antagonistas & inibidores , Cromatografia Gasosa-Espectrometria de Massas , Hemólise/efeitos dos fármacos , Humanos , Células K562 , Peroxidação de Lipídeos/efeitos dos fármacos , Malondialdeído/metabolismo , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Própole/química , Própole/toxicidadeRESUMO
Propolis is a natural mixture of compounds produced by various bee species, including stingless bees. This compound has been shown to exhibit antioxidant, antiproliferative, and antitumor activities. The present study aimed to determine the chemical constituents as well as the antioxidant, cytotoxic, and toxic activities of ethanol extracts of propolis obtained from the stingless bees Scaptotrigona depilis and Melipona quadrifasciata anthidioides, which are found in Brazil. Phytosterols, terpenes, phenolic compounds, and tocopherol were identified in the ethanol extracts of propolis (EEPs) in different concentrations. The compounds stigmasterol, taraxasterol, vanilic acid, caffeic acid, quercetin, luteolin, and apigenin were found only in EEP-M. The EEPs were able to scavenge the free radicals 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and protected human erythrocytes against lipid peroxidation, with the latter effect being demonstrated by their antihemolytic activity and inhibition of malondialdehyde formation. The EEPs showed cytotoxic activity against erythroleukemic cells and necrosis was the main mechanism of death observed. In addition, the concentrations at which the EEPs were cytotoxic were not toxic against Caenorhabditis elegans. In this context, it is concluded that EEP-S and EEP-M show antioxidant and cytotoxic activities and are promising bioactive mixtures for the control of diseases associated with oxidative stress and tumor cell proliferation.
Assuntos
Abelhas/fisiologia , Eritrócitos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Própole/farmacologia , Própole/toxicidade , Animais , Anti-Helmínticos/química , Anti-Helmínticos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Abelhas/classificação , Brasil , Caenorhabditis elegans/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Fracionamento Químico , Etanol/química , Humanos , Concentração Inibidora 50 , Células K562 , Própole/químicaRESUMO
Neste trabalho objetivou-se avaliar a ecofisiologia da germinação de sementes de Alibertia edulis (Rich) A. Rich. ex DC. (marmelo) sob diferentes condições ambientais. Dois experimentos foram realizados no Laboratório de Nutrição e Metabolismo de Plantas da UFGD. O experimento I avaliou quatro temperaturas (15, 20, 25 e 30°C) e duas condições de iluminação (presença e ausência de luz) e o experimento II o efeito de soluções salinas de KCl , NaCl e CaCl2 nos potenciais osmóticos de 0,0 (controle); -0,4; -0,8; -1,2; -1,6; -2,0 MPa na germinação das sementes. As sementes apresentam fotoblástismo neutro e não germinam a 15°C. A 25°C foi observada maior porcentagem e índice de velocidade de germinação e comprimento de parte aérea de plântulas. As sementes são sensíveis salinidade causada por NaCl, KCl e CaCl2, que afetam negativamente a germinação das sementes e no crescimento das plântulas.
The target of this study was to evaluate seed ecophysiology germination of Alibertia edulis (Rich) A. Rich. ex DC. (quince) at different environmental condition. Two experiments were done at the Laboratory of Nutrition and Metabolism of Plant from UFGD (Universidade Federal da Grande Dourados). Experiment I evaluated 4 temperatures (15, 20, 25 e 30°C) and two light conditions (absence and presence of light); experiment II evaluated the salt effect of KC1, NaCl and CaCl2 at osmotic potencial of 0,0 (control); -0,4; -0,8; -1,2; -1,6; -2,0 MPa seed germination. The seeds are photoblastism neutral and do not germinate at 15 ° C. The 25 ° C showed a higher rate of speed and percentage of germination and shoot length of seedlings. The seeds are sensitive to salinity caused by NaCl, KCl and CaCl2, which negatively affect seed germination and seedling growth.