RESUMO
Primary hyperparathyroidism is the main cause of hypercalcemia in ambulatory patients. Classic surgical resolution of this disease includes bilateral cervical exploration with exhibition of the four parathyroid glands and evaluation of the suspicious gland with a rapid biopsy. We report a 79 years old female with a primary hyperparathyroidism in whom the adenoma causing the disease was located with the help of an intraoperative gamma-probe that detected the zone of higher radiation counting of Tc99m Sestamibi, that was injected preoperatively. The patient was discharged with normal serum calcium and phosphate levels.
Assuntos
Adenoma/cirurgia , Hiperparatireoidismo/cirurgia , Neoplasias das Paratireoides/cirurgia , Compostos Radiofarmacêuticos , Tecnécio Tc 99m Sestamibi , Adenoma/diagnóstico por imagem , Idoso , Feminino , Humanos , Procedimentos Cirúrgicos Minimamente Invasivos , Neoplasias das Paratireoides/diagnóstico por imagem , Período Pós-Operatório , CintilografiaRESUMO
1. Microsomal cytochrome P-450 and peroxisomal fatty acid oxidation was studied in the kidney of rats 7 days after bile duct ligation (BDL) and a comparative study between kidney and liver was done. 2. Only in the liver did cholestasis decrease the cytocrome P-450 content and the peroxisomal fatty acid beta-oxidation, the catalase activity, and the microsomal metabolism of lauric acid and aminopyrine. 3. In contrast, cholestasis did not influence these activities in the kidney. The microsomal and peroxisomal activities studied responded in a coordinate way to cholestasis. 4. These results could suggest the possibility of a cause-and-effect relationship between microsomal cytochrome P-450 and peroxisomal activity.
Assuntos
Aminopirina/metabolismo , Catalase/metabolismo , Colestase/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Ácidos Graxos/metabolismo , Rim/metabolismo , Fígado/metabolismo , Animais , Ductos Biliares/cirurgia , Ácidos Láuricos/metabolismo , Ligadura , Masculino , Microcorpos/metabolismo , Microssomos/metabolismo , Ratos , Ratos WistarRESUMO
Microsomal cytochrome P450 and peroxisomal activity were studied in liver of rats 7 days after two-thirds hepatectomy or bile duct ligation (BDL). Both surgical models decreased the hepatic microsomal cytochrome P450 content, but only cholestasis, produced by BDL, decrease the microsomal metabolism of lauric acid and aminopyrine, peroxisomal fatty acid beta-oxidation and catalase activity. The microsomal and peroxisomal activities responded in a coordinate way to cholestasis and two-thirds hepatectomy. These results suggest a cause-effect relationship between the microsomal cytochrome P450 and peroxisomal activity.
Assuntos
Ductos Biliares/fisiologia , Ácidos Graxos/metabolismo , Fígado/fisiologia , Microcorpos/metabolismo , Microssomos Hepáticos/metabolismo , Animais , Ductos Biliares/cirurgia , Catalase/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Hepatectomia , Fígado/enzimologia , Fígado/cirurgia , Masculino , Microssomos Hepáticos/enzimologia , Oxirredução , Ratos , Ratos WistarRESUMO
Lauric acid hydroxylation and aminopyrine N-demethylation were studied in kidney and liver microsomes from rats treated with fish oil. Different doses of fish oil containing 20% eicosapentaenoic acid and 10% docosahexaenoic acid were provided daily to the rats for seven days. In all the groups studied, the lauric acid metabolism was higher in kidney microsomes and the aminopyrine metabolism in the liver microsomes. Although no effect on the renal cytochrome P-450 concentration was detectable, all four fish oil doses increased the hepatic concentration of cytochrome P-450 by a mean 27%. The higher fish oil doses used increased the renal and hepatic microsomal metabolism of aminopyrine. The lauric acid metabolism was increased by fish oil only in the liver. Fish oil, a known inducer of fatty acid peroxisomal beta-oxidation, also induced microsomal activity. These results show that liver and kidney respond in different ways to dietary factors such as fish oil. In addition, our study would suggest that fish oil increased the activity of two different families of liver cytochrome P-450. The activity of kidney lauric acid 11- and 12-hydroxylation, however, was not modulated by fish oil.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Gorduras Insaturadas na Dieta/farmacologia , Óleos de Peixe/farmacologia , Rim/enzimologia , Microssomos Hepáticos/enzimologia , Oxigenases/metabolismo , Aminopirina/metabolismo , Animais , Gorduras Insaturadas na Dieta/administração & dosagem , Ácidos Docosa-Hexaenoicos/administração & dosagem , Ácido Eicosapentaenoico/administração & dosagem , Óleos de Peixe/administração & dosagem , Rim/ultraestrutura , Ácidos Láuricos/metabolismo , Masculino , Microssomos/enzimologia , Ratos , Ratos WistarRESUMO
The catecholamines dopamine, norepinephrine and epinephrine were studied in the brains of male and female tropical bats of four species, with different feeding habits (insectivorous, frugivorous, omnivorous and pollen eater). They were trapped in a refuge at 18 degrees 24'24''N, 99 degrees 02'08''W with a mean annual temperature of 25.8 degrees C, in a tropical deciduous forest. The three catecholamines occur in both sexes of all four species, in levels which are statistically different among species as well as between sexes. Dopamine and norepinephrine levels were higher in males than females, but the opposite occurs with epinephrine. These findings suggest that changes in catecholamine levels are intimately involved in the reproductive pattern of the species studied.