1.
Sangre (Barc)
; 41(5): 379-81, 1996 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8986114
RESUMO
The transition G-->A at position 506 of the factor V gene is responsible for resistance to the anticoagulant effect of activated protein C (APC-resistance), and represents the most common hereditary risk factor for venous thrombosis. A comparison of the ability of non-radioactive single-strand conformation polymorphism (SSCP) and of a standard PCR procedure followed by further digestion with MnlI to detect this factor V gene (FVQ 506) transition indicates that these is a good agreement between the two methods. Non-radioactive SSCP analysis therefore represents a rapid and sensitive alternative for the diagnosis of this important point mutation.
Assuntos
Deficiência do Fator V/genética , Fator V/análise , Mutação Puntual , Polimorfismo Conformacional de Fita Simples , Análise Mutacional de DNA , Desoxirribonucleases de Sítio Específico do Tipo II , Suscetibilidade a Doenças , Fator V/genética , Deficiência do Fator V/complicações , Humanos , Coloração pela Prata , Tromboembolia/etiologia
2.
Thromb Haemost
; 70(2): 371, 1993 Aug 02.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7901921