RESUMEN
A simple method for preparing agar plates is presented, which allows an efficient transfer of Ureaplasma colonies to nitrocellulose membranes for subsequent immunological detection. This simple and reproducible procedure was used to demonstrate antigenic variation in the phase-variable mba-locus of Ureaplasma parvum serovar 3.
Asunto(s)
Immunoblotting/métodos , Ureaplasma/química , Variación Antigénica , Proteínas Bacterianas/análisis , Proteínas Bacterianas/inmunología , Immunoblotting/instrumentación , Ureaplasma/crecimiento & desarrollo , Ureaplasma/inmunologíaRESUMEN
Quantities of Ureaplasma spp DNA were determined by the methods of competitive and multiplex polymerase chain reactions (PCR). The results obtained by each of the methods were shown to be compatible. It was established that the number of Ureaplasma spp. cells per one epithelial cell in the female cervical canal varied from 0.05 to 10.
Asunto(s)
ADN Bacteriano/análisis , Reacción en Cadena de la Polimerasa/métodos , Ureaplasma/genética , Cartilla de ADN , ADN Bacteriano/genética , Femenino , Humanos , Fenilalanina Hidroxilasa/genética , ARN Ribosómico 16S/genética , Sensibilidad y Especificidad , Ureaplasma/química , Infecciones por Ureaplasma/microbiologíaRESUMEN
Se investigo la frecuencia de Mycoplasma hominis (M.hominis) y Ureaplasma urealyticum (U.urealyticum) en muestras obtenidas del canal endocervical de 113 mujeres que resultaron por diferentes patologías ginecológicas. Cuarenta y siete pacientes (42 por ciento) resultaron positivas a la presencia de estos dos microorganismos, siendo 26 casos (23 por ciento) positivos a M. hominis y 21 (19 por ciento; p=NS) a U. urealyticum. La edad promedio fue de 32,1 ñ
Asunto(s)
Humanos , Femenino , Antibacterianos/administración & dosificación , Bacterias/clasificación , Células/citología , Glándulas Vestibulares Mayores/anomalías , Mycoplasma fermentans/citología , Mycoplasma hominis/clasificación , Ureaplasma/químicaRESUMEN
To define the antigenic relatedness among unspeciated ureaplasma strains isolated from dogs, four canine ureaplasma isolates representing four serotypes were compared with the five type strains of the established species in Genus Ureaplasma by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Although all the strains showed distinct electrophoretic patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the Western blotting patterns were much more distinct. By Western blotting, the five type strains of established species reacted strongly with homologous antisera and showed slight cross reactions with heterologous antisera. However canine strains which showed little cross reactions with the established Ureaplasma species showed a variety of cross reactions among the four canine serotypes used.