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A simple agar plate preparation for effective transfer of Ureaplasma colonies onto nitrocellulose membranes for colony immunoblotting.
Zimmerman, Carl-Ulrich R; Stiedl, Thomas; Spergser, Joachim; Rosengarten, Renate.
Afiliación
  • Zimmerman CU; Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria. Electronic address: carl-ulrich.zimmerman@vetmeduni.ac.at.
  • Stiedl T; Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria.
  • Spergser J; Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria.
  • Rosengarten R; Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Austria.
J Microbiol Methods ; 104: 79-81, 2014 Sep.
Article en En | MEDLINE | ID: mdl-24978592
A simple method for preparing agar plates is presented, which allows an efficient transfer of Ureaplasma colonies to nitrocellulose membranes for subsequent immunological detection. This simple and reproducible procedure was used to demonstrate antigenic variation in the phase-variable mba-locus of Ureaplasma parvum serovar 3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ureaplasma / Immunoblotting Tipo de estudio: Evaluation_studies Idioma: En Revista: J Microbiol Methods Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ureaplasma / Immunoblotting Tipo de estudio: Evaluation_studies Idioma: En Revista: J Microbiol Methods Año: 2014 Tipo del documento: Article Pais de publicación: Países Bajos