RESUMEN
This study focused on two aspects: to develop a selected functionally competent bacterial community, and its integrated with biostimulant humic acid and seaweed extract which was validated to enhance wheat growth and nutrient content. Wheat and maize-associated bacterial isolates (92) were screened for Plant Growth-Promoting traits (PGPts-72) and Community-Forming traits (CFts-66). 46 isolates possessed both kinds of traits, of which 20 isolates were chosen based on high Bonitur scale ratings. Based on metabolic diversity, growth rate, and compatibility, 11 isolates were grouped to make a synthetic microbial community (SM). Non-microbial biostimulants, humic acid (HA) and seaweed extract (SWE) were used, and 0.2% HA and 1% SWE were found to be optimal for bacterial and plant growth. SM integrated each with 0.2% HA and 1% SWE, leading to products SynBio1 (SM + HA) and SynBio2 (SM + SWE). Under microcosm study, SynBio1 and SynBio2 improved germination by 90.10% and 83.80%, respectively. SynBio1 increased chlorophyll content by 40.5 SPAD units, root length by 15.7%, and shoot length by 18.4%. Field level validations revealed that SynBio1 increased plant height by 15.76%, root length by 27.16%, and flag leaf length by 21.35% compared to the control. The grain yield with SynBio1 was 40.41% higher than that of the control. Macro and micronutrient analysis of seeds treated with SynBio1 showed significant improvements. These findings demonstrate the potential of integrating microbial communities with biostimulants, and they pave the way for developing novel bioinoculants for sustainable agriculture and promoting a healthier environment.
Asunto(s)
Sustancias Húmicas , Triticum , Triticum/crecimiento & desarrollo , Sustancias Húmicas/análisis , Nutrientes/metabolismo , Algas Marinas , Bacterias/metabolismo , Clorofila/metabolismo , Zea mays/crecimiento & desarrollo , Zea mays/efectos de los fármacos , Germinación/efectos de los fármacos , Microbiología del Suelo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/microbiología , Microbiota/efectos de los fármacosRESUMEN
The mechanism for water-saving and high-yield of wide-range precision sowing technology remains unclear. We investigated the impact of wide-range precision sowing on the physiological characteristics of root system, water consumption, and grain yield of wheat 'Jimai 22' during the growing seasons of 2017-2019. We set up two planting modes: wide precision sowing and conventional strip sowing, and three row spacings of 20 cm, 25 cm, and 30 cm under water-saving cultivation with supplemental irrigation to examine the effects of planting modes on root biomass and senescence characteristics of wheat, water utilization characteristics, interplant evaporation, grain yield, and water utilization efficiency. The results showed that the 25 cm treatment (K25) led to an increase in root weight density, root soluble protein content, and root activity by 7.2%-23.9%, 8.7%-25.1%, 10.7%-29.9%, and 7.3%-27.6%, 8.0%-38.5%, 15.2%-32.7%, respectively, compared to the other treatments. At the same row spacing, the wide-range precision sowing treatment showed a significantly higher soil water storage consumption and proportion to total water consumption compared to the conventional strip-tillage treatment. Additionally, irrigation and interplant evaporation were lower in the wide-range precision sowing treatment. The K25 treatment exhibited significantly higher water consumption and modal coefficient of water consumption from flowering to ripening than other treatments. Furthermore, it had significantly higher seed yield, water utilization efficiency, and irrigation utilization efficiency than the other treatments. We found that a 25 cm spacing in the lower rows and density of 180-270 plants·m-2 was the water-saving and high-yielding planting pattern of wide-range precision sowing wheat in Huang-Huai-Hai region.
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Biomasa , Grano Comestible , Triticum , Agua , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Agua/metabolismo , Agua/análisis , Grano Comestible/crecimiento & desarrollo , Riego Agrícola/métodos , Agricultura/métodos , Producción de Cultivos/métodos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismoRESUMEN
The shortage of water resources and the irrational application of nitrogen fertilizer restrict the synergistic enhancement of yield and water- and fertilizer-use efficiencies of wheat in the Huang-Huai-Hai region. In this study, we conducted an experiment following two-factor split zone design with three irrigation levels and four nitrogen application rates. The relative water content of the 0-40 cm soil layer was supplemented to 65% (W1), 75% (W2), and 85% (W3) of field water capacity at the jointing and anthesis stages of wheat. The rates of nitrogen application were 0 (N0), 150 (N1), 180 (N2), and 210 (N3) kg·hm-2. We analyzed the effects of these different managements on post-anthesis photosynthetic matter production, yield, and water- and nitrogen-use efficiencies. The results showed that yield first increased with increases in the levels of irrigation and nitrogen application, peaking under the W2N2 treatment (9103.53 kg·hm-2). However, further increases in water and nitrogen input did not have further enhancement of wheat yield. Under the same nitrogen application condition, compared with W1 treatment, the canopy light interception rate, chlorophyll relative content and actual photochemical efficiency after anthesis increased by 4.5%-6.0%, 19.7%-28.2%, and 7.5%-9.8% in response to the W2 treatment, respectively, without any difference between the W2 and W3 irrigation levels. At the same irrigation level, post-anthesis dry matter accumulation in repose to the N2 treatment increased by 80.1%-88.9% and 16.7%-22.2% compared with N0 and N1 treatments, respectively, without significant difference between the N2 and N3 treatments. Both the irrigation water-use efficiency (IWUE) and the nitrogen partial factor productivity declined with increases in the levels of irrigation and nitrogen application. Under the W1, W2, and W3 treatments, the values obtained for IWUE were 16.23, 11.01, and 7.91 kg·hm-2·m-3, respectively, whereas in response to the N1, N2, and N3 treatments, N partial factor productivity was 50.8%, 48.4%, and 42.5%, respectively. In all, based on soil moisture measurements and assessments of wheat yield and water- and nitrogen-use efficiencies, the optimal water and nitrogen management strategy for enhancing wheat yield in the Huang-Huai-Hai region is supplementation of water content of 0-40 cm soil layer at the jointing and anthesis stages to 75% field capacity combined with the application of 180 kg·hm-2 nitrogen (W2N2). This approach could achieve high yield and efficiency and promote conservation of water and fertilizer.
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Riego Agrícola , Fertilizantes , Nitrógeno , Fotosíntesis , Triticum , Agua , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Nitrógeno/metabolismo , Agua/metabolismo , Riego Agrícola/métodos , China , BiomasaRESUMEN
Low temperature (LT) in spring usually occurs at the booting of winter wheat, resulting in reduction of wheat yield. In this study, we used the LT-sensitive wheat cultivar 'Wanmai 52' and the LT-insensitive wheat cultivar 'Yannong 19' as experimental materials to conduct LT treatment (-2 â and 0 â) at booting stage. After the LT treatment, we sprayed 6-benzylaminoadenine (6-BA) solutions with concentrations of 10, 20, and 30 mg·L-1 respectively, with equal mass distilled water as control to investigate the effects of spraying 6-BA on the physiological characteristics, yield and quality of wheat flag leaves after LT stress at booting stage. The results showed that compared with the control, young ear of wheat treated with exogenous spraying 6-BA was fuller, the floret morphology was improved, and the number of vascular bundles under the spike was increased. 6-BA application promoted the accumulation of soluble sugar, soluble protein, and proline in flag leaves. The activities of peroxidase and superoxide dismutase were increased, and the content of malondialdehyde was decreased. Exogenous 6-BA application decreased the number of degenerated spikes of wheat, increased the number of grains per spike and 1000-grain weight, as well as the contents of grain protein, wet gluten, and sedimentation value. In summary, exogenous 6-BA application could effectively alleviate the effects of LT stress on flag leaf and yield of wheat. Under the conditions of this experiment, the mitigation effect of spraying 6-BA solution on Yannong 19 was higher than that of Wanmai 52, and the mitigation effect of spraying 20 mg·L-1 6-BA solution on low temperature stress was the best.
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Frío , Hojas de la Planta , Purinas , Estrés Fisiológico , Triticum , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Triticum/efectos de los fármacos , Triticum/fisiología , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Purinas/farmacología , Biomasa , Reguladores del Crecimiento de las Plantas/farmacología , Control de Calidad , Compuestos de BenciloRESUMEN
The emergence of the Linear Pottery Culture (LBK) during the Neolithic period within Polish territory 5400-4900 BC, introduced plant cultivation, yet the definitive list of cultivated species remains debated. This study examines plant assemblages (fruits, seeds, pollen, and spores) from the LBK settlement in Biskupice, southern Poland, aiming to identify cultivated and wild species used during the development of the first stable settlements in the Carpathian Foothills. Due to extensive sampling, Biskupice yielded over 11,000 macroscopic plant specimens, enabling detailed analysis of plant diversity, distribution, and implications for agrarian and dietary practices. The analysis revealed a focus on emmer and einkorn wheat cultivation, with barley playing a minor role, alongside evidence of flax and pulses. Radiocarbon dating supported the settlement's existence in the 6th millennium BCE, and confirmed the use of barley. However, a younger date excluded bread wheat cultivation at this site. The plant assemblage included a diverse array of herbaceous wild plants like black bindweed, fat hen and brome species, suggesting their economic use. Additionally, the presence of cockspur grass, linked with Southeast Asia, indicates alternative migration routes of weeds in Europe, as supported by radiocarbon dating. Palynological analysis suggests existence of nearby cereal plots or the processing of cereals at the settlement, supporting archaeological evidence.
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Arqueología , Biodiversidad , Polonia , Humanos , Historia Antigua , Agricultura/historia , Triticum/crecimiento & desarrollo , Productos Agrícolas/crecimiento & desarrollo , Datación Radiométrica , Hordeum/crecimiento & desarrolloRESUMEN
Wheat is an important staple crop not only in Pakistan but all over the globe. Although the area dedicated to wheat cultivation expands annually, the quantity of wheat harvested is declining due to various biotic and abiotic factors. Global wheat production and output have suffered as a result of the drought, which is largely driven by a lack of water and environmental factors. Organic fertilizers have been shown to reduce the severity of drought. The current research was conducted in semi-arid climates to mitigate the negative effects of drought on wheat during its critical tillering (DTS), flowering (DFS), and grain filling (DGFS) stages through the application of three different abscisic acid treatments: ABA0 (0 mgL-1) control, ABA1 (100 mgL-1) and ABA2 (200 mgL-1). Wheat growth and yield characteristics were severely harmed by drought stress across all critical development stages, with the DGFS stage being particularly vulnerable and leading to a considerable loss in yield. Plant height was increased by 24.25%, the number of fertile tillers by 25.66%, spike length by 17.24%, the number of spikelets per spike by 16.68%, grain count per spike by 11.98%, thousand-grain weight by 14.34%, grain yield by 26.93% and biological yield by 14.55% when abscisic acid (ABA) was applied instead of the control treatment. Moreover, ABA2 increased the more physiological indices (water use efficiency (36.12%), stomatal conductance (44.23%), chlorophyll a (24.5%), chlorophyll b (29.8%), transpiration rate (23.03%), photosynthetic rate (24.84%), electrolyte leakage (- 38.76%) hydrogen peroxide (- 18.09%) superoxide dismutase (15.3%), catalase (20.8%), peroxidase (- 18.09%), and malondialdehyde (- 13.7%)) of drought-stressed wheat as compared to other treatments. In the case of N, P, and K contents in grain were maximally improved with the application of ABA2. Through the use of principal component analysis, we were able to correlate our results across scales and provide an explanation for the observed effects of ABA on wheat growth and production under arid conditions. Overall, ABA application at a rate of 200 mgL-1 is an effective technique to boost wheat grain output by mitigating the negative effects of drought stress.
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Ácido Abscísico , Sequías , Triticum , Ácido Abscísico/metabolismo , Triticum/crecimiento & desarrollo , Triticum/efectos de los fármacos , Triticum/metabolismo , Triticum/fisiología , Clorofila/metabolismo , Estrés Fisiológico , Fotosíntesis/efectos de los fármacos , Reguladores del Crecimiento de las Plantas/metabolismo , Reguladores del Crecimiento de las Plantas/farmacologíaRESUMEN
Influence of climate change on the geospatial heterogeneity in agricultural production remains poorly understood. In this study, heterogeneity in climate's impacts on wheat production across the North China Plain (NCP) was explored by integrating APSIM model, process-based factor-control quantitative approach, and geostatistical analyses. The results indicated that increased precipitation and minimum temperature boosted yields, while elevated maximum temperature and reduced radiation exerted adverse effects. The most pronounced negative impact arose from the coupling variation between maximum temperature and radiation, contributing to yields' variations of -5.84% from 2000 to 2010 and -5.22% from 2010 to 2020. In last two decades, climate change has augmented the overall geospatial heterogeneity degree in wheat yields. The chief factor contributing to yields' heterogeneity was the maximum temperature during anthesis-maturation stage, explaining an average of 37.6% of yields' heterogeneity, followed by precipitation throughout the whole growth period and the anthesis-maturation stage, explaining 36.1% and 34.5% respectively. A reciprocal enhancement mechanism exists between factors in driving yields' heterogeneity. Wheat yields in the southwestern NCP benefited more from increased precipitation and minimum temperature. Between 2000 and 2010, yields in the central NCP (junctions of Henan, Hebei, and Shandong) experienced the most pronounced adverse impact from increased maximum temperature. However, by 2010-2020, significant adverse impact shifted to western NCP, expanding spatially. During 2010-2020, the geospatial scope of radiation's significant negative impact expanded compared to the preceding decade, particularly affecting the yields in central and eastern NCP. The identified geospatial heterogeneity pattern of climate's impacts can guide spatially-matched climate-adaptive management adjustments. For instance, intensifying the defense against high-temperature's impacts in northwestern Henan, southern Hebei, and western Shandong, while improving the adaptation to radiation reduction in the central and eastern NCP. The findings are expected to advance regional-scale climate-smart agricultural development.
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Agricultura , Cambio Climático , Triticum , Triticum/crecimiento & desarrollo , China , Temperatura , ClimaRESUMEN
KEY MESSAGE: Mutations in TaCHLI impact chlorophyll levels and yield-related traits in wheat. Natural variations in TaCHLI-7A/B influence plant productivity, offering potential for molecular breeding. Chlorophyll is essential for plant growth and productivity. The CHLI subunit of the magnesium chelatase protein plays a key role inserting magnesium into protoporphyrin IX during chlorophyll biosynthesis. Here, we identify a novel wheat mutant chlorophyll (chl) that exhibits yellow-green leaves, reduced chlorophyll levels, and increased carotenoid content, leading to an overall decline in yield-related traits. Map-based cloning reveals that the chl phenotype is caused by a point mutation (Asp186Asn) in the TaCHLI-7D gene, which encodes subunit I of magnesium chelatase. Furthermore, the three TaCHLI mutants: chl-7b-1 (Pro82Ser), chl-7b-2 (Ala291Thr), and chl-7d-1 (Gly357Glu), also showed significant reductions in chlorophyll content and yield-related traits. However, TaCHLI-7D overexpression in rice significantly decreased thousand kernel weight, yield per plant, and germination. Additionally, natural variations in TaCHLI-7A/B are significantly associated with flag leaf, spike exsertion length, and yield per plant. Notably, the favorable haplotype, TaCHLI-7B-HapII, which displayed higher thousand kernel weight and yield per plant, is positively selected in wheat breeding. Our study provides insights on the regulatory molecular mechanisms underpinning leaf color and chlorophyll biosynthesis, and highlights TaCHLI functions, which provide useful molecular markers and genetic resources for wheat breeding.
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Clorofila , Liasas , Mutación Missense , Fenotipo , Hojas de la Planta , Triticum , Triticum/genética , Triticum/crecimiento & desarrollo , Clorofila/metabolismo , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/genética , Liasas/genética , Liasas/metabolismo , Metanosulfonato de Etilo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Oryza/genética , Oryza/crecimiento & desarrollo , Mapeo Cromosómico , Pigmentación/genética , Carotenoides/metabolismo , Clonación Molecular , FitomejoramientoRESUMEN
MAIN CONCLUSION: Seed-application of the natural products protects sugar beet and wheat plants against infection with plasmodiophorid-transmitted viruses and thus may represent an efficient, environmentally friendly, easy and cost effective biocontrol strategy. In times of intensive agriculture, resource shortening and climate change, alternative, more sustainable and eco-friendly plant protection strategies are required. Here, we tested the potential of the natural plant substances Glycyrrhiza glabra leaf extract (GE) and the rhamnolipid Rhapynal (Rha) applied to seeds to protect against infection of sugar beet and wheat with soil-borne plant viruses. The soil-borne Polymyxa betae- and Polymyxa graminis-transmitted viruses cause extensive crop losses in agriculture and efficient control strategies are missing. We show that GE and Rha both efficiently protect plants against infection with soil-borne viruses in sugar beet and wheat when applied to seeds. Moreover, the antiviral protection effect is independent of the cultivar used. No protection against Polymyxa sp. was observed after seed treatment with the bio-substances at our analysis time points. However, when we applied the bio-substances directly to soil a significant anti-Polymyxa graminis effect was obtained in roots of barley plants grown in the soil as well as in the treated soil. Despite germination can be affected by high concentrations of the substances, a range of antiviral protection conditions with no effect on germination were identified. Seed-treatment with the bio-substances did not negatively affect plant growth and development in virus-containing soil, but was rather beneficial for plant growth. We conclude that seed treatment with GE and Rha may represent an efficient, ecologically friendly, non-toxic, easy to apply and cost efficient biocontrol measure against soil-borne virus infection in plants.
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Beta vulgaris , Glycyrrhiza , Enfermedades de las Plantas , Extractos Vegetales , Semillas , Semillas/virología , Semillas/efectos de los fármacos , Enfermedades de las Plantas/virología , Enfermedades de las Plantas/prevención & control , Beta vulgaris/virología , Beta vulgaris/efectos de los fármacos , Extractos Vegetales/farmacología , Triticum/virología , Triticum/efectos de los fármacos , Triticum/crecimiento & desarrollo , Glucolípidos/farmacología , Virus de Plantas/fisiología , Virus de Plantas/efectos de los fármacos , Raíces de Plantas/virología , Raíces de Plantas/efectos de los fármacos , Suelo/química , Microbiología del Suelo , Hordeum/virología , Hordeum/efectos de los fármacos , Plasmodiophorida/fisiología , Plasmodiophorida/efectos de los fármacosRESUMEN
Plant growth regulators are cost-effective and efficient methods for enhancing plant defenses under stress conditions. This study investigates the ability of two plant growth-regulating substances, thiourea (TU) and arginine (Arg), to mitigate salinity stress in wheat. The results show that both TU and Arg, particularly when used together, modify plant growth under salinity stress. Their application significantly increases the activities of antioxidant enzymes while decreasing the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and relative electrolyte leakage (REL) in wheat seedlings. Additionally, these treatments significantly reduce the concentrations of Na+ and Ca2+ and the Na+/K+ ratio, while significantly increasing K+ levels, thereby preserving ionic osmotic balance. Importantly, TU and Arg markedly enhance the chlorophyll content, net photosynthetic rate, and gas exchange rate in wheat seedlings under salinity stress. The use of TU and Arg, either individually or in combination, results in a 9.03-47.45% increase in dry matter accumulation, with the maximum increase observed when both are used together. Overall, this study highlights that maintaining redox homeostasis and ionic balance are crucial for enhancing plant tolerance to salinity stress. Furthermore, TU and Arg are recommended as potential plant growth regulators to boost wheat productivity under such conditions, especially when applied together.
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Arginina , Homeostasis , Oxidación-Reducción , Estrés Salino , Plantones , Tiourea , Triticum , Triticum/metabolismo , Triticum/efectos de los fármacos , Triticum/crecimiento & desarrollo , Tiourea/farmacología , Tiourea/análogos & derivados , Arginina/metabolismo , Plantones/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Especies Reactivas de Oxígeno/metabolismo , Antioxidantes/metabolismo , Malondialdehído/metabolismo , Fotosíntesis/efectos de los fármacos , Clorofila/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismoRESUMEN
The mitigation mechanisms of a kind of controlled-release nitrogen fertilizer (sulfur-coated controlled-release nitrogen fertilizer, SCNF) in response to O3 stress on a winter wheat (Triticum aestivum L.) variety (Nongmai-88) were studied in crop physiology and soil biology through the ozone-free-air controlled enrichment (O3-FACE) simulation platform and soil microbial metagenomics. The results showed that SCNF could not delay the O3-induced leaf senescence of winter wheat but could enhance the leaf size and photosynthetic function of flag leaves, increase the accumulation of nutrient elements, and lay the foundation for yield by regulating the release rate of nitrogen (N). By regulating the soil environment, SCNF could maintain the diversity and stability of soil bacterial and archaeal communities, but there was no obvious interaction with the soil fungal community. By alleviating the inhibition effects of O3 on N-cycling-related genes (ko00910) of soil microorganisms, SCNF improved the activities of related enzymes and might have great potential in improving soil N retention. The results demonstrated the ability of SCNF to improve leaf photosynthetic function and increase crop yield under O3-polluted conditions in the farmland ecosystem, which may become an effective nitrogen fertilizer management measure to cope with the elevated ambient O3 and achieve sustainable production.
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Fertilizantes , Nitrógeno , Ozono , Fotosíntesis , Hojas de la Planta , Microbiología del Suelo , Triticum , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Triticum/microbiología , Triticum/efectos de los fármacos , Fotosíntesis/efectos de los fármacos , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Nitrógeno/metabolismo , Ozono/farmacología , Estrés Fisiológico , Suelo/química , Bacterias/efectos de los fármacos , Bacterias/metabolismo , Bacterias/genéticaRESUMEN
The lateral root (LR) is an essential component of the plant root system, performing important functions for nutrient and water uptake in plants and playing a pivotal role in cereal crop productivity. Nitrate (NO3-) is an essential nutrient for plants. In this study, wheat plants were grown in 1/2 strength Hoagland's solution containing 5 mM NO3- (check; CK), 0.1 mM NO3- (low NO3-; LN), or 0.1 mM NO3- plus 60 mg/L 2,3,5-triiodobenzoic acid (TIBA) (LNT). The results showed that LN increased the LR number significantly at 48 h after treatment compared with CK, while not increasing the root biomass, and LNT significantly decreased the LR number and root biomass. The transcriptomic analysis showed that LN induced the expression of genes related to root IAA synthesis and transport and cell wall remodeling, and it was suppressed in the LNT conditions. A physiological assay revealed that the LN conditions increased the activity of IAA biosynthesis-related enzymes, the concentrations of tryptophan and IAA, and the activity of cell wall remodeling enzymes in the roots, whereas the content of polysaccharides in the LRP cell wall was significantly decreased compared with the control. Fourier-transform infrared spectroscopy and atomic microscopy revealed that the content of cell wall polysaccharides decreased and the cell wall elasticity of LR primordia (LRP) increased under the LN conditions. The effects of LN on IAA synthesis and polar transport, cell wall remodeling, and LR development were abolished when TIBA was applied. Our findings indicate that NO3- starvation may improve auxin homeostasis and the biological properties of the LRP cell wall and thus promote LR initiation, while TIBA addition dampens the effects of LN on auxin signaling, gene expression, physiological processes, and the root architecture.
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Regulación de la Expresión Génica de las Plantas , Ácidos Indolacéticos , Nitratos , Raíces de Plantas , Transducción de Señal , Triticum , Triticum/metabolismo , Triticum/genética , Triticum/crecimiento & desarrollo , Ácidos Indolacéticos/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Raíces de Plantas/genética , Raíces de Plantas/efectos de los fármacos , Nitratos/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Pared Celular/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Organogénesis de las Plantas/genética , Perfilación de la Expresión GénicaRESUMEN
Wheat (Triticum aestivum L.) is a staple food crop that plays a crucial role in global food security. A suitable planting pattern and optimum nitrogen (N) split management are efficient practices for improving wheat production. Therefore, an experiment was performed to explore the effect of N split management and sowing patterns on wheat at the Agronomy Research Farm, The University of Agriculture Peshawar, during rabi season 2020-21 and 2021-22. The treatments consisted of different nitrogen rates of 0, 80, 120, and 160 kg ha- 1 and planting patterns of W, M, broadcast and line sowing. The pooled analysis of both cropping seasons showed that application of 120 kg N ha- 1 increased spikelets spike- 1, grains spike- 1, 1000 grains weight, grain yield, grain N content, evapotranspiration and water use efficiency by 21.9, 16.7, 21.8, 70, 13, 19.9 and 40% as compared to control, respectively. In addition, W and M were observed the best management practices among all planting patterns. The M planting pattern enhanced chlorophyll a, b, carotenoids and evapotranspiration while W plating pattern improved yield components and yield of wheat as compared to broadcast planting patterns. The principal component analysis biplot showed a close association of M and W planting patterns with 120 kg N ha- 1 in most of the studied traits. Hence, it is concluded that split application of 120 kg N ha- 1 in W and M sowing patterns enhanced growth, biochemical traits and water use efficiency, reducing N fertilization from 160 to 120 kg ha- 1 while increasing grain yield of wheat. Hence, it is recommended that application of 120 kg N ha⻹ in combination with W and M planting patterns offer a sustainable approach to enhancing wheat production in the alkaline soil conditions of the Peshawar valley.
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Fertilizantes , Nitrógeno , Triticum , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Nitrógeno/metabolismo , Fertilizantes/análisis , Producción de Cultivos/métodos , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/metabolismo , Agricultura/métodos , Clorofila/metabolismoRESUMEN
Understanding the mechanisms that regulate plant root growth under soil drying is an important challenge in root biology. We observed that moderate soil drying promotes wheat root growth. To understand whether metabolic and hormonic changes are involved in this regulation, we performed transcriptome sequencing on wheat roots under well-watered and moderate soil drying conditions. The genes upregulated in wheat roots under soil drying were mainly involved in starch and sucrose metabolism and benzoxazinoid biosynthesis. Various plant hormone-related genes were differentially expressed during soil drying. Quantification of the plant hormones under these conditions showed that the concentrations of abscisic acid (ABA), cis-zeatin (CZ), and indole-3-acetic acid (IAA) significantly increased during soil drying, whereas the concentrations of salicylic (SA), jasmonic (JA), and glycosylated salicylic (SAG) acids significantly decreased. Correlation analysis of total root length and phytohormones indicated that CZ, ABA, and IAA are positively associated with wheat root length. These results suggest that changes in metabolic pathways and plant hormones caused by moderate soil drying help wheat roots grow into deeper soil layers.
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Regulación de la Expresión Génica de las Plantas , Reguladores del Crecimiento de las Plantas , Raíces de Plantas , Suelo , Transcriptoma , Triticum , Triticum/metabolismo , Triticum/crecimiento & desarrollo , Triticum/genética , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Raíces de Plantas/genética , Reguladores del Crecimiento de las Plantas/metabolismo , Suelo/química , Ácidos Indolacéticos/metabolismo , Ácido Abscísico/metabolismo , Perfilación de la Expresión Génica/métodos , DesecaciónRESUMEN
The utilisation of coated controlled-release fertilizers (CRFs) leads to the persistence of residual plastic films in agricultural soils, posing a potential threat to crop health. This study investigates the impacts of four residual films (0.39â¯%, w/w) derived from CRFs in soil, including petrochemical polyether, bio-based polyether, castor oil polyester, and wheat straw polyester polyurethane on wheat growth. This study found that PecPEUR significantly reduced wheat plant height, stem diameter, leaf area, and aboveground fresh weight by 24.8â¯%, 20.2â¯%, and 25.7â¯%. Through an in-depth exploration of transcriptomics and metabolomics, it has been discovered that all residual films disrupted glycolysis-related metabolic pathways in wheat roots, affecting seedling growth. Among them, PecPEUR significantly reduced the fresh weight of aboveground parts by 20.5â¯%. In contrast, polyester polyurethane residue had no discernible impact on aboveground wheat growth. This was attributed to the enrichment of wheat root genes in jasmonic acid and γ-aminobutyric acid metabolic pathways, thus mitigating oxidative stress, enhancing stress resistance, and ensuring normal plant growth. This study, for the first time, provides comprehensive insights into the effects of polyurethane film residue on wheat seedling growth, underscoring its potential as a promising alternative to conventional plastics in soil.
Asunto(s)
Metabolómica , Microplásticos , Poliuretanos , Contaminantes del Suelo , Triticum , Poliuretanos/química , Triticum/efectos de los fármacos , Triticum/crecimiento & desarrollo , Triticum/genética , Contaminantes del Suelo/toxicidad , Microplásticos/toxicidad , Transcriptoma/efectos de los fármacos , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/efectos de los fármacos , Fertilizantes , Suelo/químicaRESUMEN
Heat stress poses a significant environmental challenge that profoundly impacts wheat productivity. It disrupts vital physiological processes such as photosynthesis, by impeding the functionality of the photosynthetic apparatus and compromising plasma membrane stability, thereby detrimentally affecting grain development in wheat. The scarcity of identified marker trait associations pertinent to thermotolerance presents a formidable obstacle in the development of marker-assisted selection strategies against heat stress. To address this, wheat accessions were systematically exposed to both normal and heat stress conditions and phenotypic data were collected on physiological traits including proline content, canopy temperature depression, cell membrane injury, photosynthetic rate, transpiration rate (at vegetative and reproductive stage and 'stay-green'. Principal component analysis elucidated the most significant contributors being proline content, transpiration rate, and canopy temperature depression, which exhibited a synergistic relationship with grain yield. Remarkably, cluster analysis delineated the wheat accessions into four discrete groups based on physiological attributes. Moreover, to explore the relationship between physiological traits and DNA markers, 158 wheat accessions were genotyped with 186 SSRs. Allelic frequency and polymorphic information content value were found to be highest on genome A (4.94 and 0.688), chromosome 1A (5.00 and 0.712), and marker Xgwm44 (13.0 and 0.916). Population structure, principal coordinate analysis and cluster analysis also partitioned the wheat accessions into four subpopulations based on genotypic data, highlighting their genetic homogeneity. Population diversity and presence of linkage disequilibrium established the suitability of population for association mapping. Additionally, linkage disequilibrium decay was most pronounced within a 15-20 cM region on chromosome 1A. Association mapping revealed highly significant marker trait associations at Bonferroni correction P < 0.00027. Markers Xwmc418 (located on chromosome 3D) and Xgwm233 (chromosome 7A) demonstrated associations with transpiration rate, while marker Xgwm494 (chromosome 3A) exhibited an association with photosynthetic rates at both vegetative and reproductive stages under heat stress conditions. Additionally, markers Xwmc201 (chromosome 6A) and Xcfa2129 (chromosome 1A) displayed robust associations with canopy temperature depression, while markers Xbarc163 (chromosome 4B) and Xbarc49 (chromosome 5A) were strongly associated with cell membrane injury at both stages. Notably, marker Xbarc49 (chromosome 5A) exhibited a significant association with the 'stay-green' trait under heat stress conditions. These results offers the potential utility in marker-assisted selection, gene pyramiding and genomic selection models to predict performance of wheat accession under heat stress conditions.
Asunto(s)
Estudio de Asociación del Genoma Completo , Respuesta al Choque Térmico , Triticum , Triticum/genética , Triticum/fisiología , Triticum/crecimiento & desarrollo , Respuesta al Choque Térmico/genética , Marcadores Genéticos , Fenotipo , Genotipo , Cromosomas de las Plantas/genética , Genoma de Planta , Sitios de Carácter Cuantitativo , Carácter Cuantitativo Heredable , Fotosíntesis/genéticaRESUMEN
Globally from abiotic stresses, salt stress is the major stress that limits crop production. One of them is wheat that has been utilized by more than 1/3 of the world population as staple food due to its nutritive value. Biochar is an activated carbon that can ameliorate the negative impacts on plants under saline conditions. The present study was conducted to examine the ameliorative impact of "Biochar application" to Triticum aestivum L. plant grown under salinity stress and evaluated on the basis of various growth, yield, physiological, biochemical attributes. Preliminary experiment was done to select the Triticum aestivum L. varieties with 90% germination rate for further experiment. The selected varieties, FSD08 and PUNJAB-11 of wheat were treated with two levels of sodium chloride (0 mM and 120 mM). Two varieties of wheat included FSD08 and PUNJAB-11 were treated with two levels of sodium chloride (0 mM and 120 mM). To address the impact of salt stress two levels of biochar 0% and 5% was used as exogenous application. A three way completely randomized experimentation was done in 24 pots of two wheat varieties with three replicates. The results demonstrated that salt stress affected growth, physiological attributes, yield and inorganic mineral ions (Ca2+ and K+) in roots and shoots parameters of wheat negatively while biochar overall improved the performance of plant. SOD, CAT, APX and POD activities enhanced during salt stress as the plant self-defense mechanism against salinity to minimize the damaging effect. Salt stress also significantly increased the membrane permeability, and levels of H2O2, MDA, Cl and Na ions. Biochar treatment nullified negative impacts of NaCl and improved the plant growth and yield significantly. Hence, biochar amendment can be suggested as suitable supplement for sustainable crop production under salinization.
Asunto(s)
Carbón Orgánico , Estrés Salino , Triticum , Triticum/crecimiento & desarrollo , Triticum/efectos de los fármacos , Triticum/metabolismo , Carbón Orgánico/farmacología , Germinación/efectos de los fármacos , Cloruro de Sodio/farmacología , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , SalinidadRESUMEN
Applying biochar to nitrogen (N)-fertilized soils is recognized as an effective technique for enhancing soil carbon (C) accumulation and improving agroecosystem sustainability. However, the impact of co-application of biochar and N fertilizer on soil C and N stocks, as well as their fractions, within the 0-60 cm soil profile remains unclear. This study examined the soil C and N fractions as well as stocks in soil profiles, and the primary influencing factors in wheat field with different rates of biochar (0, 20 and 40 t ha-1; B0, B1 and B2) and N application (0, 180 and 360 kg N ha-1; N0, N1 and N2). The results revealed that compared to B0N0 treatment, biochar plus N application increased soil organic carbon (SOC) and dissolved organic carbon (DOC), while N application alone decreased microbial biomass carbon (MBC). SOC in topsoil (0-10 cm) and DOC in subsoil (40-60 cm) were more susceptible to biochar and N application. The combined application of biochar and N enhanced soil N fractions, with NO3--N having the highest sensitivity than the other N fractions, whereas biochar application alone decreased topsoil inorganic N content. Biochar and N application significantly altered soil C stocks (4.33%-42.20%) and N stocks (-1.24%-20.91%) within the 0-60 cm soil layers, and belowground biomass and SOC were the main influencing factors, respectively. The combination of moderate biochar (42.35 t ha-1) and N (277.78 kg ha-1) application was the most beneficial for soil C accumulation in the 0-60 cm depth. These findings indicate the positive impacts of co-applying of biochar and N in agroecosystems on soil C and N accumulations, and highlight the importance of C and N stabilization in both topsoil and subsoil under management practice.
Asunto(s)
Carbono , Carbón Orgánico , Fertilizantes , Nitrógeno , Suelo , Triticum , Nitrógeno/análisis , Carbono/análisis , Suelo/química , Fertilizantes/análisis , Triticum/crecimiento & desarrollo , Carbón Orgánico/química , Agricultura/métodosRESUMEN
The regulation solutions and mechanisms of reducing pesticide phytotoxicity to nontarget plants are not well-defined and detailed. Here, we have proposed a new detoxification strategy to control the toxic effects of herbicide imazethapyr (IM) induced in wheat seedlings from the perspective of the plasma membrane (PM) H+-ATPase. We found that the changes in PM H+-ATPase activity have a regulatory effect on the phytotoxic effects induced by IM in plants. Treatment with PM H+-ATPase activators restored the reduced auxin content and photosynthetic efficiency caused by IM, thereby promoting plant growth. Application of a PM H+-ATPase inhibitor further reduced phosphorus content and significantly increased 2,4-dihydroxy-7-methoxy-2H,1,4-benzoxazin-3(4H)one (DIMBOA) and jasmonic acid levels. These effects indicate that auxin and DIMBOA may regulate plant growth trends and detoxification effects mediated by PM H+-ATPase. This work opens a new strategy for regulating herbicide toxicity to nontarget plants from the PM H+-ATPase.
Asunto(s)
Herbicidas , Ácidos Nicotínicos , Proteínas de Plantas , ATPasas de Translocación de Protón , Triticum , Triticum/crecimiento & desarrollo , Triticum/efectos de los fármacos , Triticum/metabolismo , Triticum/enzimología , Herbicidas/toxicidad , ATPasas de Translocación de Protón/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Ácidos Nicotínicos/toxicidad , Ácidos Nicotínicos/farmacología , Ácidos Indolacéticos/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Oxilipinas/farmacología , Ciclopentanos/farmacologíaRESUMEN
Nitrogen (N) is a key factor for plant growth and affects anthocyanin synthesis. This study aimed to clarify the potential mechanisms of N levels (LN, 0 kg·ha-1; MN, 150 kg·ha-1; HN, 225 kg·ha-1) in anthocyanin synthesis and grain quality of colored grain wheat. HN increased the yield component traits and grain morphology traits in colored grain wheat while decreasing the processing and nutrient quality traits. Most quality traits were significantly negatively correlated with the yield composition and morphological traits. Anthocyanin was more accumulated under LN conditions, but other related yield and morphological traits of colored grain wheat declined. The anthocyanin content was the highest in blue wheat, followed by that in purple wheat. Cyanidin-3-O-(6-O-malonyl-ß-d-glucoside) and cyanidin-3-O-rutinoside were the predominant anthocyanins in blue and purple wheat. The identified anthocyanin-related metabolites were associated with flavonoid biosynthesis, anthocyanin biosynthesis, and secondary metabolite biosynthesis. Therefore, the study provided information for optimizing nitrogen fertilizer management in producing high quality colored wheat and verified the close relationship between anthocyanin and low N condition.