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Nitrate Starvation Induces Lateral Root Organogenesis in Triticum aestivum via Auxin Signaling.
Tang, Chengming; Zhang, Yunxiu; Liu, Xiao; Zhang, Bin; Si, Jisheng; Xia, Haiyong; Fan, Shoujin; Kong, Lingan.
Afiliación
  • Tang C; College of Life Science, Shandong Normal University, Jinan 250014, China.
  • Zhang Y; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
  • Liu X; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
  • Zhang B; College of Life Science, Shandong Normal University, Jinan 250014, China.
  • Si J; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
  • Xia H; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
  • Fan S; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
  • Kong L; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Int J Mol Sci ; 25(17)2024 Sep 03.
Article en En | MEDLINE | ID: mdl-39273513
ABSTRACT
The lateral root (LR) is an essential component of the plant root system, performing important functions for nutrient and water uptake in plants and playing a pivotal role in cereal crop productivity. Nitrate (NO3-) is an essential nutrient for plants. In this study, wheat plants were grown in 1/2 strength Hoagland's solution containing 5 mM NO3- (check; CK), 0.1 mM NO3- (low NO3-; LN), or 0.1 mM NO3- plus 60 mg/L 2,3,5-triiodobenzoic acid (TIBA) (LNT). The results showed that LN increased the LR number significantly at 48 h after treatment compared with CK, while not increasing the root biomass, and LNT significantly decreased the LR number and root biomass. The transcriptomic analysis showed that LN induced the expression of genes related to root IAA synthesis and transport and cell wall remodeling, and it was suppressed in the LNT conditions. A physiological assay revealed that the LN conditions increased the activity of IAA biosynthesis-related enzymes, the concentrations of tryptophan and IAA, and the activity of cell wall remodeling enzymes in the roots, whereas the content of polysaccharides in the LRP cell wall was significantly decreased compared with the control. Fourier-transform infrared spectroscopy and atomic microscopy revealed that the content of cell wall polysaccharides decreased and the cell wall elasticity of LR primordia (LRP) increased under the LN conditions. The effects of LN on IAA synthesis and polar transport, cell wall remodeling, and LR development were abolished when TIBA was applied. Our findings indicate that NO3- starvation may improve auxin homeostasis and the biological properties of the LRP cell wall and thus promote LR initiation, while TIBA addition dampens the effects of LN on auxin signaling, gene expression, physiological processes, and the root architecture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triticum / Transducción de Señal / Raíces de Plantas / Regulación de la Expresión Génica de las Plantas / Ácidos Indolacéticos / Nitratos Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triticum / Transducción de Señal / Raíces de Plantas / Regulación de la Expresión Génica de las Plantas / Ácidos Indolacéticos / Nitratos Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza