RESUMEN
Sulfolobus acidocaldarius is a thermoacidophilic crenarchaeon with optimal growth at 80°C and pH 2 to 3. Due to its unique physiological properties, allowing life at environmental extremes, and the recent availability of genetic tools, this extremophile has received increasing interest for biotechnological applications. In order to elucidate the potential of tolerating process-related stress conditions, we investigated the response of S. acidocaldarius toward the industrially relevant organic solvent 1-butanol. In response to butanol exposure, biofilm formation of S. acidocaldarius was enhanced and occurred at up to 1.5% (vol/vol) 1-butanol, while planktonic growth was observed at up to 1% (vol/vol) 1-butanol. Confocal laser-scanning microscopy revealed that biofilm architecture changed with the formation of denser and higher tower-like structures. Concomitantly, changes in the extracellular polymeric substances with enhanced carbohydrate and protein content were determined in 1-butanol-exposed biofilms. Using scanning electron microscopy, three different cell morphotypes were observed in response to 1-butanol. Transcriptome and proteome analyses were performed comparing the response of planktonic and biofilm cells in the absence and presence of 1-butanol. In response to 1% (vol/vol) 1-butanol, transcript levels of genes encoding motility and cell envelope structures, as well as membrane proteins, were reduced. Cell division and/or vesicle formation were upregulated. Furthermore, changes in immune and defense systems, as well as metabolism and general stress responses, were observed. Our findings show that the extreme lifestyle of S.acidocaldarius coincided with a high tolerance to organic solvents. This study provides what may be the first insights into biofilm formation and membrane/cell stress caused by organic solvents in S. acidocaldariusIMPORTANCEArchaea are unique in terms of metabolic and cellular processes, as well as the adaptation to extreme environments. In the past few years, the development of genetic systems and biochemical, genetic, and polyomics studies has provided deep insights into the physiology of some archaeal model organisms. In this study, we used S. acidocaldarius, which is adapted to the two extremes of low pH and high temperature, to study its tolerance and robustness as well as its global cellular response toward organic solvents, as exemplified by 1-butanol. We were able to identify biofilm formation as a primary cellular response to 1-butanol. Furthermore, the triggered cell/membrane stress led to significant changes in culture heterogeneity accompanied by changes in central cellular processes, such as cell division and cellular defense systems, thus suggesting a global response for the protection at the population level.
Asunto(s)
1-Butanol/efectos adversos , Biopelículas/efectos de los fármacos , Plancton/efectos de los fármacos , Proteoma , Solventes/efectos adversos , Sulfolobus acidocaldarius/fisiología , Transcriptoma , Aclimatación , Proteínas Bacterianas/metabolismo , Genes Bacterianos , Microscopía Electrónica de Rastreo , Plancton/fisiología , Estrés Fisiológico , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/genética , Sulfolobus acidocaldarius/ultraestructuraRESUMEN
Sulfolobus acidocaldarius is the closest experimentally tractable archaeal relative of eukaryotes and, despite lacking obvious cyclin-dependent kinase and cyclin homologs, has an ordered eukaryote-like cell cycle with distinct phases of DNA replication and division. Here, in exploring the mechanism of cell division in S. acidocaldarius, we identify a role for the archaeal proteasome in regulating the transition from the end of one cell cycle to the beginning of the next. Further, we identify the archaeal ESCRT-III homolog, CdvB, as a key target of the proteasome and show that its degradation triggers division by allowing constriction of the CdvB1:CdvB2 ESCRT-III division ring. These findings offer a minimal mechanism for ESCRT-III-mediated membrane remodeling and point to a conserved role for the proteasome in eukaryotic and archaeal cell cycle control.
Asunto(s)
Proteínas Arqueales/fisiología , División Celular , Complejos de Clasificación Endosomal Requeridos para el Transporte/fisiología , Complejo de la Endopetidasa Proteasomal/fisiología , Sulfolobus acidocaldarius/citología , Proteínas Arqueales/química , Bortezomib/química , Bortezomib/farmacología , Complejos de Clasificación Endosomal Requeridos para el Transporte/química , Modelos Moleculares , Complejo de la Endopetidasa Proteasomal/química , Inhibidores de Proteasoma/química , Inhibidores de Proteasoma/farmacología , Proteolisis , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/enzimologíaRESUMEN
Several species of Sulfolobales have been used as model organisms in the study of response mechanisms to ultraviolet (UV) irradiation in hyperthermophilic crenarchaea. To date, the transcriptional responses of genes involved in the initiation of DNA replication, transcriptional regulation, protein phosphorylation, and hypothetical function have been observed in Sulfolobales species after UV irradiation. However, due to the absence of knockout experiments, the functions of these genes under in situ UV irradiation have not yet been demonstrated. In the present study, we constructed five gene knockout strains (cdc6-2, tfb3, rio1, and two genes encoding the hypothetical proteins, Saci_0951 and Saci_1302) of Sulfolobus acidocaldarius and examined their sensitivities to UV irradiation. The knockout strains exhibited significant sensitivities to UV-B irradiation, indicating that the five UV-regulated genes play an important role in responses to UV irradiation in vivo. Furthermore, Δcdc6-2, Δrio1, ΔSaci_0951, and Δtfb3 were sensitive to a wide variety of helix-distorting DNA lesions, including UV-induced DNA damage, an intra-strand crosslink, and bulky adducts. These results reveal that cdc6-2, tfb3, rio1, and Saci_0951 are play more important roles in broad responses to helix-distorting DNA damage than in specific responses to UV irradiation.
Asunto(s)
Daño del ADN/genética , Genes Arqueales/fisiología , Sulfolobus acidocaldarius/genética , Rayos Ultravioleta , 4-Nitroquinolina-1-Óxido/farmacología , Proteínas Arqueales/genética , Proteínas Arqueales/metabolismo , Cisplatino/farmacología , Reparación del ADN/genética , Técnicas de Inactivación de Genes , Genes Arqueales/genética , Metronidazol/farmacología , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/crecimiento & desarrollo , Sulfolobus acidocaldarius/efectos de la radiaciónRESUMEN
The thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius is an important model organism for Archaea and genetic systems are well established. To date, the organism is routinely cultivated on complex media based on protein hydrolysates and no common defined medium is established. In this work we address this lack of a standardized defined medium and replaced the complex protein hydrolysate with sodium glutamate as primary substrate. Starting from an existing medium formulation we stepwise managed to improve the medium regarding formation of precipitates, buffer capacity, concentration of basal salts and trace elements, and optimized growth rates. The differences on the cellular level between the original medium and our new formulation, called VD Medium, were investigated by comparative gene expression analysis and significant differences were discussed. The final formulation of the VD Medium contains 1.75 g/L Na-glutamate, 3 g/L D-glucose and 0.5 g/L citric acid as carbon sources. Using the described medium for the cultivation of S. acidocaldarius DSM 639 in shake flasks yields 1.1 g/L dry cell weight (OD600 = 1.7) after a typical incubation time of 95 h with an overall biomass yield of 0.33 gDCW/gsubstrate.
Asunto(s)
Medios de Cultivo , Sulfolobus acidocaldarius , Ácido Cítrico/metabolismo , Medios de Cultivo/química , Medios de Cultivo/metabolismo , Medios de Cultivo/farmacología , Ácido Edético/metabolismo , Ácido Glutámico/metabolismo , Concentración de Iones de Hidrógeno , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/metabolismoRESUMEN
The thermostable Phosphotriesterase-Like Lactonase from Sulfolobus solfataricus (SsoPox) hydrolyzes lactones and, at a lower rate, neurotoxic organophosphorus compounds. The persistent demand of detoxification tools in the field of agricultural wastes and restoring of conditions after terrorist acts prompted us to exploit SsoPox as a "starter" to evolve its ancillary nerve agents hydrolytic capability. A directed evolution strategy yielded, among several variants, the single mutant W263F with k(cat) and specificity constant against paraoxon 16- and 6-fold enhanced, respectively, compared to the wild type. Furthermore, a phenomenon of enzyme activation by SDS has been observed, which allowed to increase those values 150- and 28-fold, respectively. The activity of SsoPox against the deadly nerve gas Cyclosarin has been reported for the first time and proved to be substantially unaffected for variant W263F. Finally, outperforming efficiency of W263F was demonstrated, under severe stressing conditions, with respect to the best known phosphotriesterase PTE from Brevundimonas diminuta.
Asunto(s)
Hidrolasas de Éster Carboxílico/metabolismo , Sustancias para la Guerra Química/metabolismo , Sulfolobus acidocaldarius/enzimología , Sulfolobus solfataricus/enzimología , Temperatura , Biodegradación Ambiental/efectos de los fármacos , Hidrolasas de Éster Carboxílico/química , Dominio Catalítico , Evolución Molecular Dirigida , Pruebas de Enzimas , Estabilidad de Enzimas/efectos de los fármacos , Concentración de Iones de Hidrógeno/efectos de los fármacos , Hidrólisis/efectos de los fármacos , Cinética , Ligandos , Mutación/genética , Hidrolasas de Triéster Fosfórico/genética , Hidrolasas de Triéster Fosfórico/metabolismo , Estructura Secundaria de Proteína , Dodecil Sulfato de Sodio/farmacología , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus solfataricus/efectos de los fármacosRESUMEN
In contrast to the cell division machineries of bacteria, euryarchaea, and eukaryotes, no division components have been identified in the second main archaeal phylum, Crenarchaeota. Here, we demonstrate that a three-gene operon, cdv, in the crenarchaeon Sulfolobus acidocaldarius, forms part of a unique cell division machinery. The operon is induced at the onset of genome segregation and division, and the Cdv proteins then polymerize between segregating nucleoids and persist throughout cell division, forming a successively smaller structure during constriction. The cdv operon is dramatically down-regulated after UV irradiation, indicating division inhibition in response to DNA damage, reminiscent of eukaryotic checkpoint systems. The cdv genes exhibit a complementary phylogenetic range relative to FtsZ-based archaeal division systems such that, in most archaeal lineages, either one or the other system is present. Two of the Cdv proteins, CdvB and CdvC, display homology to components of the eukaryotic ESCRT-III sorting complex involved in budding of luminal vesicles and HIV-1 virion release, suggesting mechanistic similarities and a common evolutionary origin.
Asunto(s)
Archaea/citología , Archaea/fisiología , Proteínas Arqueales , División Celular/fisiología , Operón , Antibacterianos/farmacología , Archaea/clasificación , Archaea/genética , Proteínas Arqueales/genética , Proteínas Arqueales/metabolismo , División Celular/efectos de los fármacos , Análisis por Micromatrices , Sulfolobus acidocaldarius/citología , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/fisiología , Tunicamicina/farmacologíaRESUMEN
BACKGROUND: The ubiquitous Rad50 and Mre11 proteins play a key role in many processes involved in the maintenance of genome integrity in Bacteria and Eucarya, but their function in the Archaea is presently unknown. We showed previously that in most hyperthermophilic archaea, rad50-mre11 genes are linked to nurA encoding both a single-strand endonuclease and a 5' to 3' exonuclease, and herA, encoding a bipolar DNA helicase which suggests the involvement of the four proteins in common molecular pathway(s). Since genetic tools for hyperthermophilic archaea are just emerging, we utilized immuno-detection approaches to get the first in vivo data on the role(s) of these proteins in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius. RESULTS: We first showed that S. acidocaldarius can repair DNA damage induced by high doses of gamma rays, and we performed a time course analysis of the total levels and sub-cellular partitioning of Rad50, Mre11, HerA and NurA along with the RadA recombinase in both control and irradiated cells. We found that during the exponential phase, all proteins are synthesized and display constant levels, but that all of them exhibit a different sub-cellular partitioning. Following gamma irradiation, both Mre11 and RadA are immediately recruited to DNA and remain DNA-bound in the course of DNA repair. Furthermore, we show by immuno-precipitation assays that Rad50, Mre11 and the HerA helicase interact altogether. CONCLUSION: Our analyses strongly support that in Sulfolobus acidocaldarius, the Mre11 protein and the RadA recombinase might play an active role in the repair of DNA damage introduced by gamma rays and/or may act as DNA damage sensors. Moreover, our results demonstrate the functional interaction between Mre11, Rad50 and the HerA helicase and suggest that each protein play different roles when acting on its own or in association with its partners. This report provides the first in vivo evidence supporting the implication of the Mre11 protein in DNA repair processes in the Archaea and showing its interaction with both Rad50 and the HerA bipolar helicase. Further studies on the functional interactions between these proteins, the NurA nuclease and the RadA recombinase, will allow us to define their roles and mechanism of action.
Asunto(s)
Proteínas Arqueales/metabolismo , ADN Helicasas/metabolismo , ADN/metabolismo , Endodesoxirribonucleasas/metabolismo , Exodesoxirribonucleasas/metabolismo , Rayos gamma , Sulfolobus acidocaldarius/enzimología , Sulfolobus acidocaldarius/efectos de la radiación , Anticuerpos/farmacología , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Cromosomas/metabolismo , Daño del ADN , Reparación del ADN/efectos de los fármacos , Reparación del ADN/efectos de la radiación , Inmunoprecipitación , Unión Proteica/efectos de los fármacos , Unión Proteica/efectos de la radiación , Recombinasas/metabolismo , Fracciones Subcelulares/efectos de los fármacos , Fracciones Subcelulares/efectos de la radiación , Sulfolobus acidocaldarius/citología , Sulfolobus acidocaldarius/efectos de los fármacos , Factores de TiempoRESUMEN
BACKGROUND: Transcript half-lives differ between organisms, and between groups of genes within the same organism. The mechanisms underlying these differences are not clear, nor are the biochemical properties that determine the stability of a transcript. To address these issues, genome-wide mRNA decay studies have been conducted in eukaryotes and bacteria. In contrast, relatively little is known about RNA stability in the third domain of life, Archaea. Here, we present a microarray-based analysis of mRNA half-lives in the hyperthermophilic crenarchaea Sulfolobus solfataricus and Sulfolobus acidocaldarius, constituting the first genome-wide study of RNA decay in archaea. RESULTS: The two transcriptomes displayed similar half-life distributions, with medians of about five minutes. Growth-related genes, such as those involved in transcription, translation and energy production, were over-represented among unstable transcripts, whereas uncharacterized genes were over-represented among the most stable. Half-life was negatively correlated with transcript abundance and, unlike the situation in other organisms, also negatively correlated with transcript length. CONCLUSION: The mRNA half-life distribution of Sulfolobus species is similar to those of much faster growing bacteria, contrasting with the earlier observation that median mRNA half-life is proportional to the minimal length of the cell cycle. Instead, short half-lives may be a general feature of prokaryotic transcriptomes, possibly related to the absence of a nucleus and/or more limited post-transcriptional regulatory mechanisms. The pattern of growth-related transcripts being among the least stable in Sulfolobus may also indicate that the short half-lives reflect a necessity to rapidly reprogram gene expression upon sudden changes in environmental conditions.
Asunto(s)
Genes Arqueales , ARN de Archaea/genética , ARN Mensajero/genética , Sulfolobus acidocaldarius/genética , Transcripción Genética , ADN de Archaea/genética , ADN Complementario/genética , Dactinomicina/farmacología , Semivida , Cinética , Análisis de Secuencia por Matrices de Oligonucleótidos , ARN de Archaea/metabolismo , ARN Mensajero/metabolismo , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/crecimiento & desarrollo , Transcripción Genética/efectos de los fármacosRESUMEN
Type II DNA topoisomerases have been classified into two families, Topo IIA and Topo IIB, based on structural and mechanistic dissimilarities. Topo IIA is the target of many important antibiotics and antitumoural drugs, most of them being inactive on Topo IIB. The effects and mode of action of Topo IIA inhibitors in vitro and in vivo have been extensively studied for the last twenty-five years. In contrast, studies of Topo IIB inhibitors were lacking. To document this field, we have studied two Hsp90 inhibitors (radicicol and geldanamycin), known to interact with the ATP-binding site of Hsp90 (the Bergerat fold), which is also present in Topo IIB. Here, we report that radicicol inhibits the decatenation and relaxation activities of Sulfolobus shibatae DNA topoisomerase VI (a Topo IIB) while geldanamycin does not. In addition, radicicol has no effect on the Topo IIA Escherichia coli DNA gyrase. In agreement with their different effects on DNA topoisomerase VI, we found that radicicol can theoretically fit in the ATP-binding pocket of the DNA topoisomerase VI 'Bergerat fold', whereas geldanamycin cannot. Radicicol inhibited growths of Sulfolobus acidocaldarius (a crenarchaeon) and of Haloferax volcanii (a euryarchaeon) at the same doses that inhibited DNA topoisomerase VI in vitro. In contrast, the bacteria E.coli was resistant to this drug. Radicicol thus appears to be a very promising compound to study the mechanism of Topo IIB in vitro, as well as the biological roles of these enzymes in vivo.
Asunto(s)
Inhibidores Enzimáticos/farmacología , Proteínas HSP90 de Choque Térmico/antagonistas & inhibidores , Lactonas/farmacología , Inhibidores de Topoisomerasa II , Proteínas Arqueales , Benzoquinonas , Proliferación Celular/efectos de los fármacos , ADN-Topoisomerasas de Tipo II , Inhibidores Enzimáticos/química , Haloferax volcanii/citología , Haloferax volcanii/efectos de los fármacos , Haloferax volcanii/enzimología , Lactamas Macrocíclicas , Lactonas/química , Macrólidos , Quinonas/farmacología , Sulfolobus acidocaldarius/citología , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/enzimologíaRESUMEN
To investigate the generality of efficient double-strand break repair and damage-induced mutagenesis in hyperthermophilic archaea, we systematically measured the effects of five DNA-damaging agents on Sulfolobus acidocaldarius and compared the results to those obtained for Escherichia coli under corresponding conditions. The observed lethality of gamma-radiation was very similar for S. acidocaldarius and E. coli, arguing against unusually efficient double-strand break repair in S. acidocaldarius. In addition, DNA-strand-breaking agents (gamma-radiation or bleomycin), as well as DNA-cross-linking agents (mechlorethamine, butadiene diepoxide or cisplatin) stimulated forward mutation, reverse mutation, and formation of recombinants via conjugation in Sulfolobus cells. Although two of the five DNA-damaging agents failed to revert the E. coli auxotrophs under these conditions, all five reverted S. acidocaldarius auxotrophs.
Asunto(s)
Reactivos de Enlaces Cruzados/farmacología , Daño del ADN , ADN Bacteriano/efectos de los fármacos , ADN Bacteriano/efectos de la radiación , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/efectos de la radiación , Antibacterianos/farmacología , Bleomicina/farmacología , ADN/efectos de los fármacos , ADN/efectos de la radiación , Rayos gamma , Mutagénesis , Recombinación Genética , Sulfolobus acidocaldarius/genéticaRESUMEN
The genomic mutation rate of the archaeon Sulfolobus acidocaldarius, which inhabits a harsh and potentially mutagenic environment, surprisingly agrees well with the previously observed constancy of genomic mutation rates in microbes. The evolutionary explanation for this constancy of genomic mutation rates remains obscure.
Asunto(s)
Proteínas Arqueales , Evolución Molecular , Mutación , Ácido Orótico/análogos & derivados , Sulfolobus acidocaldarius/genética , Proteínas Bacterianas/genética , Genoma Arqueal , Ácido Orótico/farmacología , Sulfolobus acidocaldarius/efectos de los fármacosRESUMEN
Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombination can be assayed by conjugation and simple selections. Crosses among spontaneous pyr mutants were able to resolve closely spaced chromosomal mutations, identify deletions and rearrangements, and map mutations to a given deletion interval. Frameshift mutations in pyrE exerted polar effects that depressed orotidine-5'-monophosphate decarboxylase activity (encoded by pyrF), whereas base pair substitutions and an 18-bp deletion had no effect.
Asunto(s)
Proteínas Arqueales , Proteínas Bacterianas/metabolismo , Genes Arqueales , Sulfolobus acidocaldarius/genética , Transactivadores/metabolismo , Alelos , Proteínas Bacterianas/genética , Proteínas Bacterianas/fisiología , Conjugación Genética , Proteínas de Unión al ADN , Farmacorresistencia Microbiana , Mutación del Sistema de Lectura , Factores de Integración del Huésped , Ácido Orótico/análogos & derivados , Ácido Orótico/farmacología , Regiones Promotoras Genéticas , Pseudomonas , Recombinación Genética , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/enzimología , Temperatura , Factores de Transcripción/genética , Factores de Transcripción/metabolismoRESUMEN
Few antibiotics targeting members of the archaeal domain are currently available for genetic studies. Since bacterial antibiotics are frequently directed against competing and related organisms, archaea by analogy might produce effective antiarchaeal antibiotics. Peptide antibiotic (halocin) preparations from euryarchaeal halophilic strains S8a, GN101, and TuA4 were found to be toxic for members of the hyperthermophilic crenarchaeal genus Sulfolobus. No toxicity was evident against representative bacteria or eukarya. Halocin S8 (strain S8a) and halocin R1 (strain GN101) preparations were cytostatic, while halocin A4 (strain TuA4) preparations were cytocidal. Subsequent studies focused on the use of halocin A4 preparations and Sulfolobus solfataricus. Strain TuA4 cell lysates were not toxic for S. solfataricus, and protease (but not nuclease) treatment of the halocin A4 preparation inactivated toxicity, indicating that the A4 toxic factor must be a secreted protein. Potassium chloride supplementation of the Sulfolobus assay medium potentiated toxicity, implicating use of a salt-dependent mechanism. The utility of halocin A4 preparations for genetic manipulation of S. solfataricus was assessed through the isolation of UV-induced resistant mutants. The mutants exhibited stable phenotypes and were placed into distinct classes based on their levels of resistance.
Asunto(s)
Antibacterianos/biosíntesis , Antibacterianos/farmacología , Halobacterium/metabolismo , Péptidos , Sulfolobus/efectos de los fármacos , Péptidos Catiónicos Antimicrobianos , Medios de Cultivo , Farmacorresistencia Microbiana/genética , Halobacterium/crecimiento & desarrollo , Halobacterium salinarum/crecimiento & desarrollo , Halobacterium salinarum/metabolismo , Pruebas de Sensibilidad Microbiana/métodos , Mutación , Cloruro de Potasio/metabolismo , Sulfolobus/genética , Sulfolobus/crecimiento & desarrollo , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/genética , Sulfolobus acidocaldarius/crecimiento & desarrolloRESUMEN
Hypusination is an essential posttranslational modification unique to archaeal and eukaryotic protein synthesis initiation factor 5A (aIF5A and eIF5A, respectively). We have investigated the effect of the efficient hypusination inhibitor N(1)-guanyl-1,7-diaminoheptane (GC(7)) on four archaeal and one bacterial species. We found that (i) archaea are sensitive to GC(7), whereas the bacterium Escherichia coli is not, (ii) GC(7) causes rapid and reversible arrest of growth of the archaeon Sulfolobus acidocaldarius, and (iii) the growth arrest is accompanied by a specific reversible arrest of the cell cycle prior to cell division. Our findings establish a link between hypusination and sustained growth of archaea and thereby provide the framework to study molecular details of archaeal cell cycle in connection with in vivo functions of hypusine and of aIF5A and eIF5A.
Asunto(s)
Ciclo Celular/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Guanina/análogos & derivados , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/crecimiento & desarrollo , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Citometría de Flujo , Guanina/farmacología , Lisina/análogos & derivados , Lisina/metabolismo , Sulfolobus acidocaldarius/citologíaRESUMEN
To estimate the efficacy of mechanisms which may prevent or repair thermal damage to DNA in thermophilic archaea, a quantitative assay of forward mutation at extremely high temperature was developed for Sulfolobus acidocaldarius, based on the selection of pyrimidine-requiring mutants resistant to 5-fluoro-orotic acid. Maximum-likelihood analysis of spontaneous mutant distributions in wild-type cultures yielded maximal estimates of (2.8 +/- 0.7) x 10(-7) and (1.5 +/- 0.6) x 10(-7) mutational events per cell per division cycle for the pyrE and pyrF loci, respectively. To our knowledge, these results provide the first accurate measurement of the genetic fidelity maintained by archaea that populate geothermal environments. The measured rates of forward mutation at the pyrE and pyrF loci in S. acidocaldarius are close to corresponding rates reported for protein-encoding genes of Escherichia coli. The normal rate of spontaneous mutation in E. coli at 37 degrees C is known to require the functioning of several enzyme systems that repair spontaneous damage in DNA. Our results provide indirect evidence that S. acidocaldarius has cellular mechanisms, as yet unidentified, which effectively compensate for the higher chemical instability of DNA at the temperatures and pHs that prevail within growing Sulfolobus cells.
Asunto(s)
Calor , Mutación , Sulfolobus acidocaldarius/genética , Daño del ADN , Mutación/efectos de los fármacos , Ácido Orótico/análogos & derivados , Ácido Orótico/farmacología , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/crecimiento & desarrolloRESUMEN
The adaptive response of the archaeon Sulfolobus acidocaldarius BC65 to phosphate starvation was studied. When cells were subjected to phosphate limitation, their growth was affected. In addition, the levels of synthesis and/or the degree of phosphorylation of several proteins changed, as detected by two-dimensional nonequilibrium pH gradient electrophoresis of cells labelled in vivo with [35S]methionine and [35S]cysteine, or H3 32PO4. After another growth-restricting treatment, a heat shock, a general inhibition of protein synthesis was observed. Under phosphate starvation conditions, a 36 kDa protein became phosphorylated without its synthesis being significantly modified, suggesting a probable regulatory role during adaptation of the cell to the change in the external environment. In Southern blot analysis with specific probes from very conserved regions of the phoR and phoB genes from Escherichia coli, a positive hybridization with S. acidocaldarius BC65 chromosomal DNA fragments was found. This suggested the presence in S. acidocaldarius BC65 of genes related to the E. coli genes involved in the phosphate starvation response system. This appears to be the first evidence of the possible existence of a two-component sensory system in a micro-organism from the archaeal kingdom Crenarchaeota.
Asunto(s)
Regulación Bacteriana de la Expresión Génica , Fosfatos/farmacología , Sulfolobus acidocaldarius/fisiología , Adaptación Fisiológica , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Secuencia de Bases , ADN Bacteriano/genética , Electroforesis en Gel Bidimensional , Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Genes Bacterianos , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Fosfoproteínas/metabolismo , Fosforilación , Procesamiento Proteico-Postraduccional , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/genéticaRESUMEN
The effects of novel polyamines on aminoacyl-tRNA formation catalyzed by Escherichia coli., Sulfolobus acidocaldarius, and Thermus thermophilus HB8 S-100 extracts were investigated. These effects were diverse and differed depending on the amino acid and the enzyme used. A quaternary polyamine, tetrakis(3-aminopropyl)ammonium, inhibited phenylalanyl-tRNA synthesis catalyzed by the T. thermophilus extract, but not inhibit the other aminoacyl-tRNA formations tested. The inhibition was observed in hybrid reactions where the thermophile tRNA or extract was replaced by its E. coli counterpart, although the quaternary amine did not inhibit Phe-tRNA formation by the E. coli homologous system. Spermine relieved the inhibition of the reaction of thermophile enzyme and tRNA, but not the inhibition of the hybrid reactions. These results suggest that the branched polyamine interacts with both the thermophile enzyme and tRNA(Phe).
Asunto(s)
Poliaminas Biogénicas/farmacología , Escherichia coli/efectos de los fármacos , Compuestos de Amonio Cuaternario/farmacología , ARN de Transferencia de Fenilalanina/efectos de los fármacos , Sulfolobus acidocaldarius/efectos de los fármacos , Thermus thermophilus/efectos de los fármacos , Catálisis , Sistema Libre de Células , ARN de Transferencia de Fenilalanina/biosíntesisRESUMEN
A highly active inorganic pyrophosphatase was purified to electrophoretical homogeneity from the cytosol of Sulfolobus acidocaldarius strain 7, an extremely thermoacidophilic archaebacterium. The enzyme has an apparent molecular mass of 80 kDa as estimated by gel permeation chromatography, and showed a 21-kDa polypeptide on SDS-PAGE, suggesting that the archaebacterial enzyme is similar to most of the eubacterial pyrophosphatases rather than eukaryotic ones. The pI = 5.1. The enzyme showed relatively high content of Pro and low content of Ser plus Thr. The optimal pH was 6.5 (at 56 degrees C). From the Arrhenius plot an activation energy of 11.2 kcal/mol was obtained between 37-95 degrees C. The specific activity was 617 mumol Pi release min-1 mg-1 at 56 degrees C. The S. acidocaldarius pyrophosphatase was extremely stable. Complete activity remained after incubation at 100 degrees C for 10 min. No dissociation into subunit or unfolding of polypeptide chain occurred in the presence of 8 M urea. Experiments using guanidine-HCl suggested that the transition between a native tetrameric state and an unfolded state is completely reversible, and essentially independent of any additional factors such as divalent metal cation or dithiothreitol.
Asunto(s)
Citosol/enzimología , Pirofosfatasas/aislamiento & purificación , Sulfolobus acidocaldarius/enzimología , Aminoácidos/química , Dicroismo Circular , Difosfatos/química , Activación Enzimática/efectos de los fármacos , Estabilidad de Enzimas/efectos de los fármacos , Concentración de Iones de Hidrógeno , Punto Isoeléctrico , Peso Molecular , Pirofosfatasas/química , Especificidad por Sustrato , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/crecimiento & desarrollo , TemperaturaRESUMEN
Uracil auxotrophs of Sulfolobus acidocaldarius were positively selected by using 5-fluoroorotic acid. The wild-type strain was unable to grow in medium containing 5-fluoroorotic acid, whereas the mutants grew normally. Positive selection could be done for the auxotrophs. Mutants deficient in orotidine-5'-monophosphate pyrophosphorylase activity were isolated.
Asunto(s)
Ácido Orótico/análogos & derivados , Sulfolobus acidocaldarius/crecimiento & desarrollo , Uracilo/metabolismo , Medios de Cultivo , Genotipo , Mutación , Ácido Orótico/farmacología , Orotidina-5'-Fosfato Descarboxilasa/genética , Orotidina-5'-Fosfato Descarboxilasa/metabolismo , Especificidad de la Especie , Sulfolobus acidocaldarius/efectos de los fármacos , Sulfolobus acidocaldarius/genéticaRESUMEN
As a first step toward developing the genetic potential of extremely thermophilic archaebacteria, mutant strains of Sulfolobus acidocaldarius were selected by plating cells directly on solid medium containing one of several growth inhibitors. Three spontaneous resistance phenotypes were observed (5-fluorouracil resistance, novobiocin resistance, and L-ethionine resistance), each at a different average frequency. Characterization of representative strains showed each of the three mutant phenotypes to provide a potentially useful genetic marker.