Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase.
Bioresour Technol
; 101(23): 9204-12, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-20667718
The thermostable Phosphotriesterase-Like Lactonase from Sulfolobus solfataricus (SsoPox) hydrolyzes lactones and, at a lower rate, neurotoxic organophosphorus compounds. The persistent demand of detoxification tools in the field of agricultural wastes and restoring of conditions after terrorist acts prompted us to exploit SsoPox as a "starter" to evolve its ancillary nerve agents hydrolytic capability. A directed evolution strategy yielded, among several variants, the single mutant W263F with k(cat) and specificity constant against paraoxon 16- and 6-fold enhanced, respectively, compared to the wild type. Furthermore, a phenomenon of enzyme activation by SDS has been observed, which allowed to increase those values 150- and 28-fold, respectively. The activity of SsoPox against the deadly nerve gas Cyclosarin has been reported for the first time and proved to be substantially unaffected for variant W263F. Finally, outperforming efficiency of W263F was demonstrated, under severe stressing conditions, with respect to the best known phosphotriesterase PTE from Brevundimonas diminuta.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Temperatura
/
Hidrolasas de Éster Carboxílico
/
Sustancias para la Guerra Química
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Sulfolobus acidocaldarius
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Sulfolobus solfataricus
Idioma:
En
Revista:
Bioresour Technol
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido