RESUMEN
Salmonella serotype (ser.) Enteritidis infection in broilers is a main foodborne illness that substantially threatens food security. This study aimed to examine the effects of a novel polysaccharide isolated from alfalfa (APS) on the intestinal microbiome and systemic health of S. ser. Enteritidis-infected broilers. The results indicated that broilers receiving the APS-supplemented diet had the improved (P < 0.05) growth performance and gut health than those fed no APS-supplemented diet. Supplementation with APS enhanced (P < 0.05) the richness of gut beneficial microbes such as Bacteroidetes, Barnesiella, Parabacteroides, Butyricimonas, and Prevotellaceae, while decreased (P < 0.05) the abundance of facultative anaerobic bacteria including Proteobacteria, Actinobacteria, Ruminococcaceae, Lachnospiraceae, and Burkholderiaceae in the S. ser. Enteritidis-infected broilers. The Bacteroides and Odoribacter were identified as the two core microbes across all treatments and combined with their syntrophic microbes formed the hub in co-occurrence networks linking microbiome structure to performance of broilers. Taken together, dietary APS supplementation improved the systemic health of broilers by reshaping the intestinal microbiome regardless of whether S. ser. Enteritidis infection was present. Therefore, APS can be employed as a potential functional additives to inhibit the S. ser. Enteritidis and enhance the food safety in poultry farming.
Asunto(s)
Bacterias/clasificación , Pollos/microbiología , Medicago sativa/metabolismo , Polisacáridos/administración & dosificación , Salmonelosis Animal/dietoterapia , Salmonella enteritidis/crecimiento & desarrollo , Alimentación Animal , Animales , Bacterias/efectos de los fármacos , Bacterias/aislamiento & purificación , Pollos/inmunología , Citocinas/metabolismo , Alimentos Funcionales , Microbioma Gastrointestinal/efectos de los fármacos , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Polisacáridos/farmacología , Salmonelosis Animal/inmunología , Salmonelosis Animal/microbiología , Salmonella enteritidis/efectos de los fármacos , Análisis de Secuencia de ADN , Resultado del TratamientoRESUMEN
This study evaluated the effects of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on the physical growth, intestinal histomorphology, and blood metabolites in Salmonella-challenged birds during the finisher phase. In this study, a total of 600 chicks (Ross 308) were randomly distributed into the following dietary treatments: basal diet with no treatment; infected with Salmonella; T1, infected + avilamycin; T2, infected + Bacillus subtilis; T3, Saccharomyces boulardii; T4, infected + oregano; T5, infected + calcium montmorillonite. Our results indicated that feed consumption, body weight gain, total body weight, and feed conversion ratio increased significantly (P < 0.01) in T1 and T2. Villus width increased significantly (P < 0.01) in T1 while the total area was significantly (P < 0.01) higher in T1 and T2 among the treatment groups. Blood protein was significantly (P < 0.01) high in T3 and T4; however, the glucose concentration was significantly (P < 0.01) high in T2, T3, and T4. The treatments increased significantly (P< 0.01) in the treatment groups compared to the negative control. Aspirate aminotransferase (AST) was significantly (P < 0.05) low in T3 compared to the positive control. In conclusion, the results indicated that supplementation of Bacillus subtilis and calcium montmorillonite improved the production performance compared to other feed additives in broiler chicks infected with Salmonella during the finisher phase.
Asunto(s)
Bentonita/farmacología , Pollos/crecimiento & desarrollo , Intestinos/efectos de los fármacos , Origanum , Salmonelosis Animal/dietoterapia , Alimentación Animal/análisis , Animales , Bacillus subtilis , Sangre/metabolismo , Sangre/microbiología , Peso Corporal/efectos de los fármacos , Calcio/farmacología , Pollos/metabolismo , Pollos/microbiología , Intestinos/microbiología , Intestinos/patología , Oligosacáridos/farmacología , Enfermedades de las Aves de Corral/microbiología , Probióticos/farmacología , Saccharomyces boulardii , Salmonelosis Animal/metabolismo , Salmonella enterica/patogenicidad , Hormonas Tiroideas/sangreRESUMEN
Black soldier fly (Hermetia illucens) larvae (BSFL) are rich in protein and have the potential to be used in animal feed. The aim of the present study was to determine the immunoprophylactic effect of BSFL against Salmonella Gallinarum in broiler chicks as an alternative feed additive. Results showed that BSFL improved body weight gain and increased frequency of CD4+ T lymphocyte, serum lysozyme activity, and spleen lymphocyte proliferation. Moreover, BSFL reinforced bacterial clearance and increased survivability of broiler chicks against S. Gallinarum. These data suggested that BSFL has prophylactic properties with stimulating non-specific immune responses, as well as reduced bacterial burden against S. Gallinarum.
Asunto(s)
Alimentación Animal , Pollos , Enfermedades de las Aves de Corral/dietoterapia , Salmonelosis Animal/dietoterapia , Simuliidae , Animales , Linfocitos T CD4-Positivos/inmunología , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/microbiología , Profilaxis Pre-Exposición , Salmonelosis Animal/inmunología , Salmonelosis Animal/microbiología , Simuliidae/inmunologíaRESUMEN
Feed additives, including prebiotics, are commonly used alternatives to antimicrobial growth promoters to improve gut health and performance in broilers. Wheat bran is a highly concentrated source of (in)soluble fiber which is partly degraded by the gut microbiota. The aim of the present study was to investigate the potential of wheat bran as such to reduce colonization of the cecum and shedding of Salmonella bacteria in vivo. Also, the effect of particle size was evaluated. Bran with an average reduced particle size of 280µm decreased levels of cecal Salmonella colonization and shedding shortly after infection when compared to control groups and groups receiving bran with larger particle sizes. In vitro fermentation experiments revealed that bran with smaller particle size was fermented more efficiently, with a significantly higher production of butyric and propionic acid, compared to the control fermentation and fermentation of a larger fraction. Fermentation products derived from bran with an average particle size of 280µm downregulated the expression of hilA, an important invasion-related gene of Salmonella. This downregulation was reflected in an actual lowered invasive potential when Salmonella bacteria were pretreated with the fermentation products derived from the smaller bran fraction. These data suggest that wheat bran with reduced particle size can be a suitable feed additive to help control Salmonella infections in broilers. The mechanism of action most probably relies on a more efficient fermentation of this bran fraction and the consequent increased production of short chain fatty acids (SCFA). Among these SCFA, butyric and propionic acid are known to reduce the invasion potential of Salmonella bacteria.
Asunto(s)
Alimentación Animal/análisis , Fibras de la Dieta/análisis , Tamaño de la Partícula , Salmonelosis Animal/dietoterapia , Alimentación Animal/microbiología , Animales , Proteínas Bacterianas/genética , Derrame de Bacterias , Ciego/microbiología , Pollos , Dieta/veterinaria , Fibras de la Dieta/administración & dosificación , Ácidos Grasos Volátiles/metabolismo , Regulación Bacteriana de la Expresión Génica , Salmonella/fisiología , Salmonelosis Animal/microbiología , Transactivadores/genéticaRESUMEN
An in vitro and in vivo study was conducted to evaluate the fermentability of isolated galactoglucomannan oligosaccharides (GGMs) and the influence of their feeding on shedding and colonisation of Salmonella typhimurium, growth performance and intestinal morphology in broiler chicks. The in vitro data demonstrated that three probiotic lactic acid bacteria namely Lactobacillus casei, L. plantarum and Enterococcus faecium were able to ferment the extracted oligosaccharides and other tested sugars on a basal de Man Rogosa Sharpe media free from carbohydrate. For the in vivo experiment, 144 one-d-old male Ross 308 broiler chicks were divided into 6 experimental treatments (with 4 replicates) including two positive and negative controls which received a basal maize-soybean diet without any additives, supplementation of three levels of isolated GGMs (0.1%, 0.2% and 0.3%) and a commercial mannanoligosaccharide (MOS) at 0.2% to the basal diet. All birds except those in the negative control group were challenged orally with 1 × 108 cfu of S. typhimurium at 3-d post-hatch. The results revealed that challenge with S. typhimurium resulted in a significant reduction in body weight gain, feed intake, villus height, villus height to crypt depth ratio and villus surface area in all of infected chicks. Birds that were given GGMs or MOS showed better growth performance, increased villus height and villus surface area and decreased S. typhimurium colonisation than the positive control birds. GGM at 0.2% level was more effective than the other treatments in improving growth rate as well as gut health of broiler chicks.
Asunto(s)
Pollos , Mananos/metabolismo , Enfermedades de las Aves de Corral/dietoterapia , Probióticos , Salmonelosis Animal/dietoterapia , Alimentación Animal/análisis , Animales , Pollos/anatomía & histología , Pollos/crecimiento & desarrollo , Dieta/veterinaria , Enterococcus faecium/fisiología , Intestinos/anatomía & histología , Lacticaseibacillus casei/fisiología , Lactobacillus plantarum/fisiología , Masculino , Mananos/administración & dosificación , Pinus/química , Enfermedades de las Aves de Corral/microbiología , Probióticos/administración & dosificación , Distribución Aleatoria , Salmonelosis Animal/microbiología , Salmonella typhimurium/fisiología , Madera/químicaRESUMEN
Diarrhoea in piglets by Salmonella and other pathogens can be a serious health problem. Non-drug treatments such as probiotic microorganisms have various effects on the gastrointestinal microbiota dysbiosis and host immune system modulation. The aim of this study was to demonstrate the suitable use of Weissella paramesenteroides WpK4 strain isolated from healthy piglets as an alternative prophylactic or therapeutic treatment against Salmonella Typhimurium. Out of 37 lactic acid bacteria isolates, 24 strains belonging to the Weissella and Lactobacillus genera were analysed in vitro for desirable probiotic characteristics. The W. paramesenteroides WpK4 strain fulfilled all in vitro tests: resistance to acidic pH and bile salts, hydrophobic cell surface, antagonism against bacterial pathogens, H2O2 production and exopolysaccharide secretion, and non-transferable resistance to antibiotics. Mice fed with WpK4 showed no signs of bacterial translocation to the liver or spleen and decreased Salmonella translocation to these organs. Significantly, WpK4 intake attenuated the weight loss, fostered the preservation of intestinal architecture and integrity, and promoted survival in mice following infection with Salmonella Typhimurium. In addition, WpK4 modulated immune cellular response by inhibiting the production of pro-inflammatory cytokines and inducing anti-inflammatory mediators. These findings validate the probiotic properties of W. paramesenteroides WpK4 strain, and its eventual use in piglets.
Asunto(s)
Citocinas/genética , Probióticos/uso terapéutico , Salmonelosis Animal/dietoterapia , Porcinos/microbiología , Fiebre Tifoidea/dietoterapia , Weissella , Animales , Peso Corporal , Citocinas/metabolismo , Modelos Animales de Enfermedad , Femenino , Expresión Génica , Inmunoglobulina A/biosíntesis , Intestinos/inmunología , Intestinos/microbiología , Masculino , Ratones , Salmonella typhimurium , Weissella/aislamiento & purificaciónRESUMEN
This study was conducted to examine the effects of fructooligosaccharide (FOS) supplementation on growth performance, lymphoid organ weight, intestinal morphology, and immunological status in broilers (n=180) challenged with Salmonella Enteritidis lipopolysaccharides (LPS). Birds were randomly assigned into a 3×2 factorial arrangement that included 1) 3 dietary treatments from d one to 21: positive control (PC), wheat-corn-soybean meal based diet contained antibiotics (virginiamycin and monensin); negative control (NC), as PC without antibiotics; and NC+FOS, as NC supplemented with 0.5% FOS, and 2) 2 intraperitoneal injections: 2 mg/kg Salmonella Enteritidis LPS or sterile phosphate buffered saline (PBS) on d 21. Growth performance and relative lymphoid organ weight were not significantly different among the treatments. Villus height, crypt depth, and total mucosa thickness were significantly increased (P<0.05) in the ileum of broiler chickens fed NC+FOS when compared to PC and NC. Birds in NC+FOS treatment had reduced heterophil but increased monocyte count when compared to NC (P<0.05). Significant diet×challenge interaction was observed on natural IgY levels (P<0.0001), and a significant dietary effect was observed on specific IgY levels in chickens fed NC+FOS (P=0.003). Supplementation of FOS also increased the expression of interleukin (IL)-1ß, -10, and interferon (IFN)-γ mRNA in the ileum of the birds. In summary, Salmonella Enteritidis LPS challenge established significant differences in the immune responses in broiler chickens. FOS supplementation increased ileal mucosa thickness and elevated the expressions of certain cytokine genes. It also led to the alteration of leukocyte compositions and serum IgY levels in response to LPS challenge, suggesting FOS supplementation may be effective to induce protective outcomes in gut health and immunity of broiler chickens.
Asunto(s)
Pollos , Inmunidad Innata/efectos de los fármacos , Oligosacáridos/farmacología , Enfermedades de las Aves de Corral/dietoterapia , Salmonelosis Animal/dietoterapia , Salmonella enteritidis/fisiología , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos , Animales , Dieta/veterinaria , Suplementos Dietéticos/análisis , Lipopolisacáridos/administración & dosificación , Masculino , Oligosacáridos/administración & dosificación , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/microbiología , Salmonelosis Animal/inmunología , Salmonelosis Animal/microbiologíaRESUMEN
The dietary supplementation of Saccharomyces cerevisiae var. boulardii was evaluated in broilers challenged or not challenged with Salmonella Enteritidis (SE) using a 2 × 2 factorial arrangement. Depending on yeast inclusion at 0 (C) or 1 × 109 cfu/kg diet (Y) and SE challenge (0 or log 6.3 cfu/bird) on d 15, the experiment had four treatments C, Y, C-SE, and Y-SE, respectively. Each treatment had seven replicate floor pens with 15 broilers. Growth performance responses were determined weekly and overall for the 5 week experimental period. Salmonella levels and prevalence in ceca, cloacae, and carcass skin were determined by culture procedures, while cecal microbiota was determined by real time PCR. Yeast supplementation had no effect (PY > 0.05) on growth performance. For the overall post SE-challenge period (i.e., wk 3 to wk 5), Salmonella reduced body weight gain (BWG) (PSE < 0.001), feed intake (FI) (PSE = 0.032), and the European production efficiency (EPEF) factor (PSE = 0.005). Broilers Y-SE had higher (P < 0.001) overall BW gain compared to C-SE ones. Overall mortality was 2.14% and did not differ (P > 0.05) between treatments. Reduced Salmonella levels in the cloacae (P = 0.014) and on the breast skin (P = 0.006) and lower prevalence on the neck skin (P = 0.007) were noted for treatment Y-SE compared to C-SE. Yeast supplementation did not have an effect (P > 0.05) on cecal microbiota composition at d 1 and d 21 post SE-challenge. On the contrary, SE-challenge reduced cecal levels of total bacteria (PSE = 0.002), E. coli (PSE = 0.006), Bifidobacterium spp. (PSE = 0.006), Bacteroides spp. (PSE = 0.010), and Clostridial populations belonging to cluster I and cluster XIVa, (PSE = 0.047 and PSE = 0.001, respectively) on d 1 post SE-challenge. At 21 d post SE-challenge, only the levels of cecal Lactobacillus spp. (PSE = 0.001) and Bifidobacterium spp. (PSE = 0.049) were reduced compared to the non SE-challenged groups. In conclusion, yeast supplementation in SE challenged broilers (Y-SE) was beneficial for growth performance and reduced Salmonella presence compared to C-SE ones. The disturbance of cecal microbiota balance by SE merits further investigation for potential implications in gut and overall bird health.
Asunto(s)
Pollos , Enfermedades de las Aves de Corral/dietoterapia , Salmonelosis Animal/dietoterapia , Salmonella enteritidis/aislamiento & purificación , Levadura Seca/farmacología , Alimentación Animal/análisis , Animales , Ciego/microbiología , Cloaca/microbiología , Dieta/veterinaria , Suplementos Dietéticos/análisis , Contenido Digestivo/microbiología , Masculino , Microbiota/efectos de los fármacos , Enfermedades de las Aves de Corral/microbiología , Distribución Aleatoria , Salmonelosis Animal/microbiología , Piel/microbiología , Levadura Seca/administración & dosificaciónRESUMEN
This study assessed the effect of both embryonic thermal manipulation and dietary threonine level on the response of broilers inoculated with Salmonella Enteritidis, considering bacterial counts in the cecal contents, intestinal morphology, mucin and heat shock protein 70 gene expression, body weight and weight gain. Thermal manipulation was used from 11 days of incubation until hatch, defining three treatments: standard (37.7°C), continuous high temperature (38.7°C) and continuous low temperature (36.7°C). After hatch, chicks were distributed according to a 3x2+1 factorial arrangement (three temperatures and two threonine levels and one sham-inoculated control). At two days of age, all chicks were inoculated with Salmonella Enteritidis, except for the sham-inoculated control group. There was no interaction between the factors on any analyses. High temperature during incubation was able to reduce colonization by Salmonella Enteritidis in the first days, reducing both Salmonella counts and the number of positive birds. It also increased mucin expression and decreased Hsp70 expression compared with other inoculated groups. High temperature during incubation and high threonine level act independently to reduce the negative effects associated to Salmonella Enteritidis infection on intestinal morphology and performance, with results similar to sham-inoculated birds. The findings open new perspectives for practical strategies towards the pre-harvest Salmonella control in the poultry industry.
Asunto(s)
Pollos/inmunología , Dieta/veterinaria , Íleon/inmunología , Enfermedades de las Aves de Corral/inmunología , Salmonelosis Animal/inmunología , Treonina/administración & dosificación , Animales , Peso Corporal/efectos de los fármacos , Peso Corporal/inmunología , Embrión de Pollo , Pollos/genética , Pollos/crecimiento & desarrollo , Pollos/microbiología , Recuento de Colonia Microbiana , Regulación de la Expresión Génica , Proteínas HSP70 de Choque Térmico/genética , Proteínas HSP70 de Choque Térmico/inmunología , Íleon/metabolismo , Íleon/microbiología , Mucina 2/genética , Mucina 2/inmunología , Enfermedades de las Aves de Corral/dietoterapia , Enfermedades de las Aves de Corral/genética , Enfermedades de las Aves de Corral/microbiología , Salmonelosis Animal/dietoterapia , Salmonelosis Animal/genética , Salmonelosis Animal/microbiología , Salmonella enteritidis/inmunología , Temperatura , Aumento de Peso/efectos de los fármacos , Aumento de Peso/inmunologíaRESUMEN
AIMS: To assess the efficacy of a ß-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. METHODS AND RESULTS: Three different doses (0.5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out in a small fattening unit (≈ 100 pigs). Treatment was randomly assigned to half of the pens. Individual serum samples (20-25 per group) were collected at different times during the fattening period and a similar number of faecal samples during the fattening period and at slaughter. In addition, mesenteric lymph nodes were collected at slaughter. Herdcheck(®) Swine Salmonella ELISA was used for serological analyses, the ISO 6579:2002/Amd 1 : 2007 for bacteriology and the PFGE for molecular characterization of Salmonella strains. The addition of ≥ 2 kg t(-1) of ß-GMOS to the pig diet during the entire fattening period was associated with a reduction in Salmonella prevalence, shedding and seroconversion. CONCLUSIONS: Feed supplementation with ß-GMOS may be a useful complementary tool for the control of salmonellosis in fattening pigs. SIGNIFICANCE AND IMPACT OF THE STUDY: ß-GMOS may be a complementary way of reducing Salmonella shedding and infection in fattening pigs.
Asunto(s)
Suplementos Dietéticos , Mananos/administración & dosificación , Salmonelosis Animal/dietoterapia , Enfermedades de los Porcinos/dietoterapia , Animales , Derrame de Bacterias , Galactosa/análogos & derivados , Mananos/uso terapéutico , Oligosacáridos/administración & dosificación , Oligosacáridos/uso terapéutico , Salmonella/clasificación , Salmonella/inmunología , Salmonella/aislamiento & purificación , Salmonelosis Animal/microbiología , Estudios Seroepidemiológicos , Porcinos , Enfermedades de los Porcinos/microbiologíaRESUMEN
Salmonellosis is an insidious and potentially epidemic problem in pre-weaned dairy calves. Managing this disease, or any other diarrheal disease, is a financial burden to producers. Calf mortalities and medicinal treatments are overt costs of salmonellosis, while hidden costs include hampered weight gains and persistent intestinal colonization of the pathogen. In this study, we examined the anti-Salmonella effects of Saccharomyces cerevisiae fermentation products (SCFP) incorporated into both the milk replacer and the starter grain. In a blinded study, 2-8 day-old calves were fed SCFP (n=20 calves) or an SCFP-free Control (n=20 calves) for two weeks before and three weeks after experimental challenge with Salmonella enterica serotype Typhimurium. Following the challenge, calves were monitored for clinical signs and parameters associated with salmonellosis. Calves were then euthanized and examined for rumen development and intestinal Salmonella colonization. When compared to calves that received milk replacer and feed lacking SCFP, calves fed SCFP had fewer bouts of diarrhea and fever. Rumens from these calves were more developed, as measured by the length of papillae, which is consistent with the enhanced weight gain observed in this treatment group. Additionally, Salmonella intestinal colonization was reduced in SCFP-fed calves and Salmonella fecal shedding disappeared at an earlier stage in these calves. This study revealed that the combination of two proprietary S. cerevisiae fermentation products provide marked benefit for preventing the negative effects of salmonellosis in pre-weaned dairy calves, while also boosting productivity. The mechanism of action needs to be clarified, but it may be related to the observed decrease in colonization by the pathogen and increase in rumen development.
Asunto(s)
Enfermedades de los Bovinos/dietoterapia , Diarrea/dietoterapia , Sustitutos de la Leche/administración & dosificación , Saccharomyces cerevisiae/química , Salmonelosis Animal/dietoterapia , Alimentación Animal , Animales , Bovinos , Enfermedades de los Bovinos/microbiología , Diarrea/microbiología , Diarrea/veterinaria , Heces/microbiología , Fermentación , Masculino , Rumen/efectos de los fármacos , Rumen/crecimiento & desarrollo , Rumen/microbiología , Saccharomyces cerevisiae/metabolismo , Salmonelosis Animal/microbiología , Salmonella enterica/patogenicidad , Salmonella enterica/fisiología , Destete , Aumento de PesoRESUMEN
Reducing Salmonella in poultry has been a paramount goal of the poultry industry in order to improve food safety. Inclusion of high-fiber fermentable feedstuffs in chicken diets has been shown to reduce the incidence of Salmonella colonization in laying hens, but no work has been performed in growing birds. Therefore, the present study was designed to quantify differences in artificially inoculated cecal Salmonella Typhimurium populations in growing layer chicks (n = 60 in each of two replications) fed 0%, 25%, and 50% of their diet (w/w) replaced with alfalfa meal from day (d) 7 to d14 after hatch. Alfalfa supplementation reduced cecal populations of Salmonella by 0.95 and 1.25 log10 colony-forming unit per gram in the 25% and 50% alfalfa groups compared to controls. Alfalfa feeding reduced (p < 0.05) the number of cecal- and crop-positive birds compared to controls. Increasing levels of alfalfa increased (p < 0.05) total volatile fatty acids (VFA) and the proportion of acetate in the cecum. Surprisingly, alfalfa inclusion did not negatively impact average daily gain (ADG) in birds over the 7-d feeding period. Alfalfa inclusion at 50% of the diet increased (p < 0.05) the number of bacterial genera detected in the cecum compared to controls, and also altered proportions of the microbial population by reducing Ruminococcus and increasing Clostridia populations. Results support the idea that providing a fermentable substrate can increase gastrointestinal VFA production and bacterial diversity which in turn can reduce colonization by Salmonella via natural competitive barriers. However, further studies are obviously needed to more fully understand the impact of changes made in diet or management procedures on poultry production.
Asunto(s)
Alimentación Animal , Pollos/microbiología , Medicago sativa , Salmonelosis Animal/dietoterapia , Salmonella typhimurium/crecimiento & desarrollo , Animales , Enfermedades de las Aves/dietoterapia , Enfermedades de las Aves/microbiología , Enfermedades de las Aves/prevención & control , Ciego/microbiología , Recuento de Colonia Microbiana , Ácidos Grasos Volátiles/análisis , Femenino , Fermentación , Contaminación de Alimentos/prevención & control , Microbiología de Alimentos , Incidencia , Masculino , Salmonelosis Animal/microbiología , Salmonelosis Animal/prevención & control , Salmonella typhimurium/aislamiento & purificación , Células MadreRESUMEN
A Salmonelose é uma importante zoonose, considerada a principal causa de infecções bacterianas, sendo associada ao consumo de produtos avícolas. Como alternativa de controle, ácidos orgânicos têm sido amplamente usados. No entanto, pouco se conhece sobre o estado imunológico de aves de produção, e uma avaliação deste status é necessária para proteger frente a enfermidades e para garantir à aplicação segura de agentes terapêuticos ou imunização profilática. Este trabalho teve como objetivo verificar o comportamento do sistema imunológico das aves previamente infectadas com Salmonella Enteritidis (SE) tratadas com um composto de ácidos orgânicos em diferentes concentrações administrado via água e ração comparando com as aves infectadas e não tratadas. Foram inoculados 120 frangos de corte com 1mL de SE, via oral, na concentração de 1,0 x 108 UFC/mL, no 1º e 2º dia de idade, divididos em seis tratamentos com duas repetições, utilizando 200, 400, 500 e 1000ppm do ácido orgânico. Aos 35 dias de vida das aves, foram coletados, de todos os grupos, alíquotas de sangue de 3mL em tubo contendo EDTA para a avaliação das células imunes através de citometria de fluxo. Foram analisadas as porcentagens circulantes de células CD4+, CD8β+, MHC I+, MHC II+, TCRVβ1+, TCRVβ2+ e CD28+. Para análise microbiológica foram coletadas tonsilas cecais destas aves. Observou-se com esse estudo que os ácidos orgânicos nas dosagens 1000ppm na água e 500ppm na ração durante, dois e sete dias respectivamente antes do abate, foram eficazes na redução da infecção por SE em frangos de corte, comprovadas pelo método microbiológico e demonstradas através do comportamento das células do sistema imune. No presente estudo as aves infectadas apresentaram uma proporção menor de células T auxiliares circulantes quando comparadas às aves infectadas, mas tratadas com o AO ou com o grupo não infectado. A mesma tendência pode ser observada para as células CD28+, TCRVβ1+ e MHC IIbright+, e, com menor resolução, para CD8β+.
Salmonellosis is an important zoonosis, considered the leading cause of bacterial infections, and is associated with the consumption of poultry products. As alternative control, organic acids have been widely used. However, little is known about the immune status of poultry production, and an evaluation of this status is necessary to protect against disease and to ensure the safe application of therapeutic agents or prophylactic vaccination. This study aimed to verify the behavior of the immune system of birds previously infected with Salmonella Enteritidis (SE) treated with a compound of organic acids in different concentrations administered via water and food, compared with the infected birds and untreated. One hundred and twenty broilers were orally inoculated with 1ml of SE at a concentration of 1.0x108 CFU/mL, at 1 and 2-days-old and divided into six treatments with two repetitions of 200, 400, 500 and 1000ppm organic acid. From 35-days-old birds of all groups were collected aliquots of 3mL of blood into a tube containing EDTA for the evaluation of immune cells by flow cytometry. We then analyzed the percentages of circulating CD4+, CD8β+, MHC I+ MHC II+, TCRVβ1+, CD28+ + and TCRVβ2. For microbiological analysis were collected caecal tonsils of these birds. We found that organic acids in dosages 1000ppm 500ppm in water and in feed for 2 to 7 days before slaughter, respectively, were effective in reducing SE infection in broilers, proven by microbiological method and demonstrated through the behavior of immune cells. The infected birds showed a lower proportion of circulating T helper cells compared with infected poultry, but treated with AO or with the uninfected group. The same trend can be observed for CD28+ cells, and MHC IIbright+ TCRVβ 1+, and with lower resolution, for CD8β+.
Asunto(s)
Animales , Aves de Corral/inmunología , Aves de Corral/microbiología , Ácidos Orgánicos , Salmonella enteritidis/aislamiento & purificación , Salmonelosis Animal/dietoterapia , Salmonelosis Animal/prevención & control , Autopsia , Ácido Ascórbico/administración & dosificación , Ácido Cítrico/uso terapéutico , Ácido Láctico/administración & dosificaciónRESUMEN
AIMS: To screen from pickled vegetables the potential probiotic lactic acid bacteria (LAB) strains with antagonistic activity against Salmonella invasion in host. METHODS AND RESULTS: Probiotic properties including acid and bile tolerance as well as inhibition on pathogenic bacteria were used for screening of LAB strains from pickled vegetables. Two strains, i.e Pediococcus pentosaceus MP12 and Lactobacillus plantarum LAP6, were selected and further assayed for their activities against Salmonella invasion in mouse liver and spleen. For these two LAB strains, strain LAP6 was able to adhere to the mouse intestinal epithelium cells. CONCLUSIONS: In screening of the probiotic strains able to inhibit the Salmonella invasion in host, factors other than the adherence to host intestinal epithelium may contribute some roles. SIGNIFICANCE AND IMPACT OF THE STUDY: Probiotic LAB strains with activity against Salmonella invasion in host could be isolated from vegetable origins. These strains may be used for vegetable processing.
Asunto(s)
Conservación de Alimentos , Lactobacillaceae/aislamiento & purificación , Probióticos , Salmonelosis Animal/dietoterapia , Verduras , Animales , Antibiosis , Adhesión Bacteriana , Células Cultivadas , Recuento de Colonia Microbiana , Mucosa Intestinal/microbiología , Lactobacillus plantarum/aislamiento & purificación , Hígado/microbiología , Ratones , Ratones Endogámicos BALB C , Pediococcus/aislamiento & purificación , Salmonelosis Animal/microbiología , Bazo/microbiologíaRESUMEN
In an experiment with broilers infected with a rifampicin-resistant Salmonella Typhimurium strain at 15 days of age, it was demonstrated that whole wheat feeding influenced the course of infection. The pH in the contents of the gizzard decreased as the amount of whole wheat in the diet increased over time. Following infection, lower numbers of Salmonella Typhimurium were found in the gizzard and ileum of birds receiving whole wheat compared to pellet-fed birds. However, there was no difference with respect to the numbers of Salmonella Typhimurium in the ceca and rectum between the two experimental groups. Quantitative cultivation of different groups of bacteria belonging to the natural intestinal microflora showed that whole wheat feeding significantly reduced the numbers of Clostridium perfringens in the intestinal tract of the birds. The present results indicate that the gizzard has an important function as a barrier organ, one that prevents pathogenic bacteria from entering the distal digestive tract.
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Pollos , Tracto Gastrointestinal/microbiología , Enfermedades de las Aves de Corral/dietoterapia , Enfermedades de las Aves de Corral/microbiología , Salmonelosis Animal/dietoterapia , Salmonella typhimurium , Triticum , Crianza de Animales Domésticos/métodos , Animales , Clostridium perfringens , Recuento de Colonia MicrobianaRESUMEN
In three experiments the effects of prophylactic or therapeutic dietary inclusion of capsaicin, the pungent component of peppers, were evaluated as a nonantibiotic alternative for reduction of Salmonella in broiler chickens through culture and morphologic assessment of cecal tissue. Expt. 1 evaluated the effects of 0 or 10 ppm purified capsaicin (CAP) in the starter phase (days 1-16) on chicks challenged with Salmonella Enteritidis (SE) on day of age. Therapeutic inclusion of 10 ppm purified CAP increased (P < 0.05) liver/spleen (L/S) and ceca positive results for SE. In Expt. 2, capsaicin oleoresin (CO) was included in the finisher diet (days 30-37) at 0, 5, or 20 ppm with SE challenge on day 31. Inclusion of 5 ppm CO increased ceca positive results for SE, and a linear decrease in cecal lamina propria thickness of SE-challenged birds was observed with increased CO concentration in the diet. Expt. 3 evaluated prophylactic CO treatment at 0, 5, or 20 ppm in starter, grower, and finisher diets for resistance to SE or Salmonella Typhimurium (ST) challenge on day 14 or 29. With challenge on day 14, 5 and 20 ppm prophylactic CO feeding reduced ceca SE positive results by 37% and 26%, respectively, and ST culture rate was reduced similarly with 5 ppm CO. Lamina propria thickness of the ceca increased with 5 ppm CO feeding in SE-challenged birds, whereas a decrease was observed in nonchallenged birds fed 5 ppm CO. Challenge on day 29 of birds fed 20 ppm CO resulted in reduced L/S positive results for SE. Lamina propria thickness decreased with 5 ppm CO and SE or ST challenge compared with nonchallenged birds fed 5 ppm. An increase was observed in ST- or SE-challenged birds fed 20 ppm CO compared with nonchallenged birds fed 20 ppm CO. No differences were observed in mast cell number in either Expt. 2 or 3. These data provide evidence that prophylactic or therapeutic dietary capsaisin differentially affects broiler susceptibility to Salmonella.
Asunto(s)
Capsaicina/administración & dosificación , Pollos/microbiología , Enfermedades de las Aves de Corral/prevención & control , Salmonelosis Animal/prevención & control , Alimentación Animal , Animales , Capsaicina/uso terapéutico , Ciego/microbiología , Hígado/microbiología , Enfermedades de las Aves de Corral/dietoterapia , Enfermedades de las Aves de Corral/microbiología , Salmonelosis Animal/dietoterapia , Salmonelosis Animal/microbiología , Salmonella enteritidis/aislamiento & purificación , Salmonella enteritidis/patogenicidad , Salmonella typhimurium/aislamiento & purificación , Salmonella typhimurium/patogenicidad , Bazo/microbiologíaRESUMEN
BACKGROUND: Protection against intestinal infections by lactulose fermentation might be enhanced by calcium phosphate, due to the increased buffering capacity and/or inhibition of the cytotoxicity of luminal contents. METHODS: SPF rats were fed a low-calcium control diet, a low-calcium/lactulose diet or a high-calcium/lactulose diet and orally infected with Salmonella enteritidis. Faeces and urine were sampled for microbiological and chemical analyses. RESULTS: The lactulose-fed rats had a better colonization resistance. Translocation was reduced by dietary calcium, whereas lactulose was ineffective. In addition, calcium supplementation stimulated lactulose fermentation, reversed the lactulose-induced increase in cytotoxicity of faecal water and normalized growth and faecal consistency. CONCLUSIONS: The combination of dietary lactulose and calcium phosphate is protective against Salmonella infection.
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Fosfatos de Calcio/uso terapéutico , Calcio de la Dieta/uso terapéutico , Fármacos Gastrointestinales/uso terapéutico , Lactulosa/uso terapéutico , Salmonelosis Animal/prevención & control , Salmonella enteritidis , Animales , Traslocación Bacteriana , Heces/química , Heces/microbiología , Fermentación , Ratas , Salmonelosis Animal/dietoterapia , Salmonella enteritidis/fisiología , Organismos Libres de Patógenos EspecíficosRESUMEN
Providing direct-fed-microbial (DFM) bacteria and fructooligosaccharides (FOS) for the control of potential escalation of Salmonella colonization during simulated feed withdrawal and confinement was assessed. Eight hundred and eighty broilers (16 pens; 55 chicks per pen) were reared to 6 wk of age. Chicks were sprayed with a solution containing 10(6) nalidixic-acid resistant Salmonella typhimuriumNR cells per milliliter on the 2nd d after hatching. Because this first challenge did not yield a high infection rate, chickens were rechallenged per Os at Day 18 by providing water containing 10(7) cells of S. typhimuriumNR per milliliter. At 3 and 5 wk of age, 10 birds per pen were euthanatized and cecal Salmonella were quantified (log colony-forming units per gram). Feed was removed from all pens at 6 wk, and pens were randomly assigned to be either the treatment group or the control group. The treatment groups were provided a DFM (mixture of nine bacteria) and FOS 50 (R) (10%) in the drinking water. The control groups received drinking water only. After 6 h of feed withdrawal, chickens were cooped (eight per coop) and held 10 h. Immediately after confinement, 10 chickens were used for cecal enumeration of S. typhimuriumNR. Salmonella colonization declined from 99% at 3 wk to 44% at 5 wk. After feed withdrawal, application of the treatment, and confinement, 11 and 14% of the treated and control groups, respectively, yielded S. typhimuriumNR by direct plating from ceca (3.87 and 3.75 log 10 cfu/g, respectively). No difference (P > 0.05) in Salmonella colonization occurred between the treated and the control groups; however, enrichment of ceca (incubation in nutrient broth at 37 C for 24 h) yielded a higher incidence of S. typhimuriumNR in the control groups (32% in the treated vs 51% in the control). Ceca weights were greater in the treated group (P < 0.05). Simulated feed withdrawal and confinement did not escalate Salmonella colonization in the chicken ceca.
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Dieta/veterinaria , Privación de Alimentos/fisiología , Lactobacillus/fisiología , Oligosacáridos/farmacología , Enfermedades de las Aves de Corral/prevención & control , Salmonelosis Animal/prevención & control , Salmonella/crecimiento & desarrollo , Animales , Ciego/microbiología , Pollos , Dieta/normas , Ingestión de Líquidos/fisiología , Ingestión de Alimentos/fisiología , Enterococcus faecium/aislamiento & purificación , Enterococcus faecium/fisiología , Lactobacillus/aislamiento & purificación , Lactococcus lactis/aislamiento & purificación , Lactococcus lactis/fisiología , Oligosacáridos/administración & dosificación , Pediococcus/aislamiento & purificación , Pediococcus/fisiología , Enfermedades de las Aves de Corral/dietoterapia , Enfermedades de las Aves de Corral/fisiopatología , Propionibacterium/aislamiento & purificación , Propionibacterium/fisiología , Salmonella/aislamiento & purificación , Salmonelosis Animal/dietoterapia , Salmonelosis Animal/fisiopatología , Salmonella typhimurium/aislamiento & purificación , Microbiología del AguaRESUMEN
The effect of dietary lactose and Lactobacillus acidophilus on the colonization of Salmonella enteritidis (phage type 4) in newly hatched leghorn chicks concurrently infected with Eimeria tenella was studied. There were six groups of birds in each of two replicate trials: uninfected controls, birds infected with S. enteritidis, birds infected with S. enteritidis and E. tenella, birds infected with S. enteritidis and E. tenella that were fed with dietary lactose (5%), birds infected with S. enteritidis and E. tenella that were provided with L. acidophilus, and birds infected with S. enteritidis and E. tenella that were fed both L. acidophilus and dietary lactose (5%). Chickens were necropsied at 2, 5, and 8 days after S. enteritidis inoculation for bacteriological examination. The cecal population of S. enteritidis was significantly increased by infection with E. tenella. Lactose alone and the combination of lactose and L. acidophilus significantly reduced the population of S. enteritidis in the ceca of birds infected with E. tenella (P < 0.05), but the combination was more effective than lactose alone (P < 0.05). Adding anaerobic culture of L. acidophilus alone did not significantly decrease the population of S. enteritidis in the ceca. No significant reduction of colonization of S. enteritidis in the liver and spleen of birds with coccidiosis was observed.
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Coccidiosis/dietoterapia , Coccidiosis/veterinaria , Eimeria tenella/efectos de los fármacos , Lactobacillus acidophilus/fisiología , Lactosa/uso terapéutico , Salmonelosis Animal/dietoterapia , Salmonella enteritidis/crecimiento & desarrollo , Animales , Animales Recién Nacidos/microbiología , Animales Recién Nacidos/parasitología , Ciego/microbiología , Pollos , Eimeria tenella/fisiología , Hígado/microbiología , Salmonella enteritidis/efectos de los fármacos , Bazo/microbiologíaRESUMEN
Mice received rations containing a mixture of lard with sunflower oil, butter, cooking fat (hydrogenated), fish fat. Hydrogenated fat and fish fat induced a decrease in the portion of polyunsaturated fatty acid omega 6 in lipids of lymphocytes, Peyer's patches, mesenteric lymph nodes and the spleen. At the same time fish fat induced an increase of the portion of polyunsaturated fatty acid omega 3 in lipids studied. A relationship has been noted between the animal survival after intraperitoneal infection with S. Typhimurium and the fatty acid composition in lymphoid tissues. The highest survival rate has been recorded in the groups of animals that received cooking (hydrogenated) fat and fish fat.