RESUMEN
At present, there are few reports on the glycosylation modification of soybean proteins under irradiation. In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure. The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed. When the irradiation dose was 7.5 kGy, the solubility of modified products increased by 23 ± 0.21% compared with that of the control group. The foaming property and foam stability increased by 62.5 ± 0.34% and 41 ± 0.47%, respectively. Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly. Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement. This experiment combined irradiation technology with the glycosylation modification method. It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.
Asunto(s)
Rayos gamma , Proteínas de Soja , Manipulación de Alimentos , Glicosilación , Interacciones Hidrofóbicas e Hidrofílicas , Reacción de Maillard , Maltosa/química , Solubilidad , Proteínas de Soja/análisis , Proteínas de Soja/química , Proteínas de Soja/efectos de la radiaciónRESUMEN
The effect of power, time and temperature of ultrasound on the structure of ß-conglycinin (7S) and glycinin (11S), and on the antioxidant activity of their hydrolysates were investigated. All ultrasound treated 7S and 11S fractions showed an increase in the α-helix and ß-turn proportions, and a decrease in ß-sheet and random coil proportions. The polarity of 7S and 11S microenvironment increased after ultrasound treatment. Ultrasound treatment significantly increased the reduction capacity and iron chelating capacity of 7S and 11S hydrolysates. The degree of hydrolysis and free SH groups of 7S and 11S hydrolysates increased after ultrasound pre-treatment. The relative content of high molecular weight peptides reduced, and the relative content of low molecular weight peptides increased in ultrasound treated 7S and 11S hydrolysates. The ultrasonication exposed certain groups of 7S and 11S fractions, improved contact with enzymes, and increased the content of highly active soybean antioxidant peptides.
Asunto(s)
Antígenos de Plantas/química , Antioxidantes/metabolismo , Globulinas/química , Glycine max/química , Extractos Vegetales/química , Proteínas de Almacenamiento de Semillas/química , Proteínas de Soja/química , Ultrasonido , Antígenos de Plantas/efectos de la radiación , Antioxidantes/análisis , Globulinas/efectos de la radiación , Oxidación-Reducción , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/efectos de la radiación , Proteínas de Almacenamiento de Semillas/efectos de la radiación , Proteínas de Soja/efectos de la radiación , TemperaturaRESUMEN
The aim of this study was to investigate the structural characteristics and antioxidant activities of soy protein isolate- (SPI-) dextran conjugates obtained by TiO2 photocatalysis treatment. Results revealed that the UV-vis absorption and the fluorescence intensity increased as the photocatalytic power increased (P < 0.05). Higher photocatalytic power could promote the extent of glycation and the formation of high molecular weight SPI-dextran conjugates, which were evidenced by free amino group content and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The Fourier transform infrared (FT-IR) spectra suggested that the amide I, II, and III bands of SPI were altered by the glycation induced by TiO2 photocatalysis. Moreover, significant changes of secondary structure occurred in SPI-dextran conjugates. The α-helix, ß-sheet, ß-turns, and random coil were changed from approximately 10.6%, 37.9%, 12.9%, and 38.6% to 3.8%, 10.4%, 17.7%, and 68.8%, respectively, after treatment at photocatalytic power of 1000 W. In addition, SPI-dextran conjugates obtained by TiO2 photocatalysis treatment exhibited high hydroxyl radical scavenging activity and possessed increased reducing power. All data indicated that TiO2 photocatalysis was an efficient method for promoting protein-polysaccharide copolymerisation.
Asunto(s)
Antioxidantes/síntesis química , Dextranos/química , Nanoconjugados/química , Proteínas de Soja/química , Titanio/química , Antioxidantes/análisis , Catálisis , Dextranos/efectos de la radiación , Luz , Nanoconjugados/efectos de la radiación , Fotoquímica/métodos , Proteínas de Soja/efectos de la radiación , Titanio/efectos de la radiaciónRESUMEN
This study investigated pulsed ultraviolet (PUV) illumination at different distances from the PUV source on soybean lipoxygenase (LOX) (0.4 mg/mL in 0.01 M Tris-HCl buffer, pH 9) activity. Samples (5 mL) were illuminated for 1, 2, 4, 8, and 16 s at 3 distances 6, 8.5, and 11 cm from the PUV lamp's quartz window. The temperature of 33.5 ± 1.8°C was observed for the highest treatment time of 16 s at the shortest distance of 6 cm, and resulted in a 3.5 log reduction (99.95%) in initial LOX activity. Illumination time and distance from the lamp significantly (P ≤ 0.05) affected LOX inactivation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed on treated LOX samples and further protein profile for treated LOX filtrate (≤10 kDa), was analyzed by reverse phase high-performance liquid chromatography (RP-HPLC). The protein profile analysis revealed that LOX protein degradation was influenced significantly (P ≤ 0.05) by PUV illumination time.
Asunto(s)
Glycine max/enzimología , Lipooxigenasa/efectos de la radiación , Rayos Ultravioleta , Electroforesis en Gel de Poliacrilamida , Proteínas de Soja/efectos de la radiación , TemperaturaRESUMEN
Novel chitosan (cts) and soybean protein isolate (SI) blended membranes were prepared. These membranes were produced by solvent casting. Besides combining the advantages of both materials, cts/SI membranes exhibit a biphasic structure that will eventually originate in situ porous formation, through a two-step degradation mechanism. In this particular work the effect of beta-radiation over the properties of these membranes was evaluated. beta-radiation sterilisation was performed at three different doses (25, 50 and 100 kGy) and eventual surface chemical changes were evaluated by Fourier transformed infrared--with attenuated total reflection and contact angle measurements. Moreover, eventual bulk properties changes due to beta-radiation were assessed by means of mechanical tensile tests and water uptake measurements. In general, no substantial changes were detected on the studied properties, with the exception of the surface energy that was found to be slightly increased for higher applied doses.
Asunto(s)
Partículas beta , Regeneración Ósea , Quitina/análogos & derivados , Quitina/química , Quitina/efectos de la radiación , Proteínas de Soja/química , Proteínas de Soja/efectos de la radiación , Esterilización/métodos , Materiales Biocompatibles/química , Materiales Biocompatibles/efectos de la radiación , Quitosano , Elasticidad/efectos de la radiación , Ensayo de Materiales , Membranas Artificiales , Peso Molecular , Conformación Proteica/efectos de la radiación , Propiedades de Superficie , Resistencia a la Tracción/efectos de la radiación , Ingeniería de Tejidos/métodos , Agua/químicaRESUMEN
Sterilized biofilms based on soy protein isolate (SPI, S system) and a 1:1 mixture of SPI and whey protein isolate (WPI, SW system) were achieved through the formation of cross-links by means of gamma-irradiation combined with thermal treatments. The effect of the incorporation of carboxymethylcellulose (CMC) and poly(vinyl alcohol) was also examined. gamma-Irradiation combined with thermal treatment improved significantly the mechanical properties, namely, puncture strength and puncture deformation, for all types of films. Irradiated formulations that contain CMC behave more similarly as elastomers. CMC showed also significant improvements of the barrier properties, namely, water vapor permeability, for irradiated films of the S system and for non-irradiated films of the SW system.
Asunto(s)
Proteínas de la Leche/química , Proteínas de Soja/química , Carboximetilcelulosa de Sodio/química , Rayos gamma , Calor , Proteínas de la Leche/efectos de la radiación , Alcohol Polivinílico/química , Proteínas de Soja/efectos de la radiación , Proteína de Suero de LecheRESUMEN
Protein solubility (PS) values of different soy protein isolate (SPI) films were determined in water, 0.01 N HCl, 0.01 N NaOH, 4 M urea, and 0.2 M 2-mercaptoethanol. Tensile and color (L, a, and b values) properties of films also were determined. Control films were cast from heated (70 degrees C for 20 min), alkaline (pH 10) aqueous solutions of SPI (5 g/100 mL of water) and glycerin (50% w/w of SPI). Additional films were cast after incorporation of dialdehyde starch (DAS) at 10% w/w of SPI or small amounts of formaldehyde in the film-forming solutions. Also, control film samples were subjected to heat curing (90 degrees C for 24 h), UV radiation (51.8 J/m(2)), or adsorption of formaldehyde vapors. PS of control films was highest (P < 0.05) in 2-mercaptoethanol, confirming the importance of disulfide bonds in SPI film formation. All treatments were effective in reducing (P < 0.05) film PS in all solvents. Both DAS and adsorbed formaldehyde rendered the protein in films practically insoluble in all solvents. Adsorption of formaldehyde vapors and heat curing also substantially increased (P < 0.05) film tensile strength from 8.2 to 15.8 or 14.7 MPa, respectively. However, heat curing decreased (P < 0.05) film elongation at break from 30 to 6%. Most treatments had small but significant (P < 0.05) effects on b color values, with DAS-containing films having the greatest (P < 0. 05) mean b value (most yellowish). Also, DAS-containing, heat-cured, and UV-irradiated films were darker, as evidenced by their lower (P < 0.05) L values, than control films. It was demonstrated that PS of SPI films can be notably modified through chemical or physical treatments prior to or after casting.