RESUMEN
OBJECTIVE: To investigate the biological mechanisms underlying the effect of the Chinese herbal medicine Oxalis corniculata on human prostate cancer PC-3 cells. METHODS: Through in vitro experiment, we treated human prostate cancer PC-3 cells with different concentrations of Oxalis corniculata, assessed the viability of the cells by MTT assay, examined their apoptosis by flow cytometry, evaluated their migration and invasiveness by Transwell assay, and determined the expressions of the proteins p65, p-p65, IκBα and p-IκBα in the NF-κB pathway using protein imprinting technology. RESULTS: Compared with the blank control, Oxalis corniculata significantly inhibited the proliferation and induced the apoptosis of the PC-3 cells (P< 0.05), suppressed their migration and invasiveness in a dose-dependent manner (P< 0.05), and upregulated the expression of IκBα and downregulated those of p-p65 and p-IκBα in the NF-κB pathway (P< 0.05). CONCLUSION: Oxalis corniculata can inhibit the proliferation, migration and invasiveness and induce the apoptosis of human prostate cancer PC cells, which may be attributed to its abilities of inhibiting the expressions of p-p65 and p-IκBα and regulating the activity of the NF-κB pathway.
Asunto(s)
Oxalidaceae , Neoplasias de la Próstata , Masculino , Humanos , FN-kappa B/metabolismo , Inhibidor NF-kappaB alfa/farmacología , Células PC-3 , Oxalidaceae/metabolismo , Neoplasias de la Próstata/metabolismo , ApoptosisRESUMEN
It has been reported that the aqueous extract from Oxalis corniculate has excellent pharmacological effects, but its polysaccharide as the major ingredient in the aqueous extract has not been reported. When the temperature of 50°C, ultrasonic power of 270 W, time of 25 min, solid to liquid ratio of 30 ml·g-1 , the optimal O. corniculate polysaccharide (OCP) yield was 9.45%. The physicochemical properties indicated that OCP-3, as the major fraction of OCP, was an acidic polysaccharide with 31.5 kDa, and it mainly consisted of arabinose (47.83%), galacturonic acid (17.81%), and galactose (14.25%). In addition, OCP-3 displayed an excellent antioxidant activity in vitro, including scavenging free radical, anti-lipid peroxidation, and protecting plasmid DNA from oxidative damage. Meanwhile, OCP-3 significantly reduced the levels of malondialdehyde and protein carbonyl by significantly increasing the activity of superoxide dismutase, catalase, and glutathione peroxidase, which protected the HEK 293 cell and Caenorhabditis elegans from oxidative damage. All the results suggested that OCP-3 might be the major active ingredient of the aqueous extract from O. corniculate, and OCP-3 might be a potent antioxidant supplement in the food, cosmetics, and medical industries. PRACTICAL APPLICATIONS: Oxalis corniculate is a kind of wild vegetable and ethnomedicine, and it is widely distributed in temperate zones. Unfortunately, its utilization rate is low compared to its yield. Our research suggested that the polysaccharide of OCP-3 from O corniculate might be used as a potent antioxidant supplement in the food, cosmetics, and medical industries.
Asunto(s)
Antioxidantes , Oxalidaceae , Ácidos , Antioxidantes/química , Células HEK293 , Humanos , Peroxidación de Lípido , Oxalidaceae/química , Oxalidaceae/metabolismo , Polisacáridos/química , Polisacáridos/farmacologíaRESUMEN
The objective of this study was to profile the chemical components and biological activity analysis of crude extract of Bryophyllum pinnatum and Oxalis corniculata. Results revealed that the analyzed plant materials encompass the high amount of total phenolic and flavonoids content and have significant antioxidant activities. Furthermore, methanol extracts are the potential source of α-amylase, α-glucosidase, lipase, tyrosinase and elastase inhibitors. High resolution mass spectrometry revealed the presence of diverse metabolites such as quercetin 3-O-α-L-rhamnopyranoside, myricetin 3-rhamnoside, bersaldegenin 1,3,5-orthoacetate, bryophyllin C, syringic acid, caffeic acid, p-coumaric acid, and quercetin in B. pinnatum and isoorientin, swertisin, apigenin 7,4'-diglucoside, vitexin, 4-hydroxybenzoic acid, vanillic acid, ethyl gallate, 3,3',4'-trihydroxy-5,7-dimethoxyflavone, and diosmetin-7-O-ß-D-glucopyranoside in O. corniculata. Our finding suggested that these two plant species have high medicinal importance and are potential source of inhibitors for modern pharmaceuticals, nutraceuticals and cosmetics industries.
Asunto(s)
Inhibidores Enzimáticos/química , Kalanchoe/química , Oxalidaceae/química , Extractos Vegetales/química , Cromatografía Líquida de Alta Presión , Inhibidores Enzimáticos/metabolismo , Flavonoides/química , Flavonoides/metabolismo , Kalanchoe/metabolismo , Monofenol Monooxigenasa/antagonistas & inhibidores , Monofenol Monooxigenasa/metabolismo , Nepal , Oxalidaceae/metabolismo , Fenoles/química , Fenoles/metabolismo , Extractos Vegetales/metabolismo , Espectrometría de Masa por Ionización de Electrospray , alfa-Amilasas/antagonistas & inhibidores , alfa-Amilasas/metabolismo , alfa-Glucosidasas/química , alfa-Glucosidasas/metabolismoRESUMEN
Oca (Oxalis tuberosa) is an underutilized species and represents a novel starch source. Composition and structure of starches from tubers of two commercial oca varieties grown in New Zealand were compared to those of normal maize and potato starches. The phosphorus content of oca starch was â¼60% of that of potato starch. The amylose content of oca starch (â¼21%) was lower than that of maize and potato starches (concanavalin A precipitation method). The fine structure of oca amylopectin was much more similar to that of potato amylopectin than to that of maize amylopectin. Oca amylopectin had a shorter internal chain length and less fingerprint B-chains than potato amylopectin. The two oca starches were structurally and compositionally similar. Oca starch granules had a volume moment mean size of 34.5⯵m and B-type polymorph. Comparative analysis suggested that oca starch has the potential to be developed as a novel starch source.
Asunto(s)
Oxalidaceae/metabolismo , Solanum tuberosum/metabolismo , Almidón/química , Zea mays/metabolismo , Amilopectina/análisis , Amilosa/análisis , Cromatografía Líquida de Alta Presión , Microscopía Electrónica de Rastreo , Nueva Zelanda , Fósforo/análisis , Difracción de Rayos XRESUMEN
The present study investigated remediation of mercury-contaminated soils using Oxalis corniculata L. combined with various enhancers (sodium thiosulfate, ammonium thiosulfate, ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid). The experiment was conducted using Oxalis corniculata seedlings planted in pots containing mercury loaded soils. Investigations included analysis of soil properties, plant growth conditions, ability of the plants to accumulate and extract mercury, and rhizosphere microorganism distribution. The maximal mercury content of the aerial parts and the mercury-translocation ratio of Oxalis corniculata treated with enhancers increased compared to Oxalis corniculata without enhancers. Compared with no enhancers, the theoretical reduction in phytoremediation time was about 50%, 25%, 20% and 21% when Oxalis corniculata was treated with sodium thiosulfate (Na2S2O3), ammonium thiosulfate ((NH4)2S2O3), ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA), respectively. The results indicated that the dominant species in rhizosphere soils varied with different enhancers. However, the evenness of background soils, rhizosphere soils of Oxalis corniculata, Oxalis corniculata treated with Na2S2O3, (NH4)2S2O3, EDTA and DTPA was not largely different at 0.62, 0.61, 0.57, 0.64, 0.61 and 0.63, respectively. These findings demonstrate that Oxalis corniculata treated with Na2S2O3 has the potential to recover and reclaim mercury-contaminated soils in pots.