RESUMEN
BACKGROUND: The horn fly, Haematobia irritans irritans, causes significant production losses to the cattle industry. Horn fly control relies on insecticides; however, alternative control methods such as vaccines are needed due to the fly's capacity to quickly develop resistance to insecticides, and the pressure for eco-friendly options. METHODS: We used a reverse vaccinology approach comprising three vaccine prediction and 11 annotation tools to evaluate and rank 79,542 translated open reading frames (ORFs) from the horn fly's transcriptome, and selected 10 transcript ORFs as vaccine candidates for expression in Pichia pastoris. The expression of the 10 selected transcripts and the proteins that they encoded were investigated in adult flies by reverse transcription polymerase chain reaction (RT-PCR) and mass spectrometry, respectively. Then, we evaluated the immunogenicity of a vaccine candidate in an immunization trial and the antigen's effects on horn fly mortality and fecundity in an in vitro feeding assay. RESULTS: Six of the ten vaccine candidate antigens were successfully expressed in P. pastoris. RT-PCR confirmed the expression of all six ORFs in adult fly RNA. One of the vaccine candidate antigens, BI-HS009, was expressed in sufficient quantity for immunogenicity and efficacy trials. The IgG titers of animals vaccinated with BI-HS009 plus adjuvant were significantly higher than those of animals vaccinated with buffer plus adjuvant only from days 42 to 112, with a peak on day 56. Progeny of horn flies feeding upon blood from animals vaccinated with BI-HS009 plus adjuvant collected on day 56 had 63% lower pupariation rate and 57% lower adult emergence than the control group (ANOVA: F (1, 6) = 8.221, P = 0.028 and F (1, 6) = 8.299, P = 0.028, respectively). CONCLUSIONS: The reverse vaccinology approach streamlined the discovery process by prioritizing possible vaccine antigen candidates. Through a thoughtful process of selection and in vivo and in vitro evaluations, we were able to identify a promising antigen for an anti-horn fly vaccine.
Asunto(s)
Enfermedades de los Bovinos/prevención & control , Inmunogenicidad Vacunal , Muscidae/genética , Muscidae/inmunología , Vacunas/inmunología , Vacunología/métodos , Animales , Antígenos/genética , Antígenos/inmunología , Bovinos , Femenino , Masculino , Reacción en Cadena de la Polimerasa/métodos , Transcripción ReversaRESUMEN
Thrombostasin (TS) is an anticlotting protein found in saliva of Haematobia irritans (horn flies). The polymorphic nature of the ts gene was first associated with success of horn flies blood feeding on a laboratory host, New Zealand White rabbits. In this study, we report results of similar studies testing blood uptake of horn flies feeding on a natural host, cattle. These studies confirmed the association of ts genotype with blood uptake of horn flies and showed that it was host species specific. In contrast to rabbits, blood uptake volumes of homozygous ts10 horn flies were lower than those of other ts genotypes when fed on control (ovalbumin-vaccinated) cattle. Cattle vaccinated with recombinant protein isoforms, rTS9 or rTB8, resisted horn fly feeding by yielding lower blood volumes compared with flies feeding on control cattle. The specific impact of vaccination, however, varied by ts genotype of flies. Cattle vaccinated with isoform rTS9 resisted flies of ts2, ts9, and tb8 genotype. Vaccination with isoform rTB8 produced resistance to ts8, ts9, and tb8 genotype flies. Horn flies of genotype ts10 were not affected by vaccination with either TS isoform and fed as well on rTS9- and rTB8-vaccinated as on control-vaccinated cattle. These experimental results confirm the efficacy of vaccines targeting horn fly salivary proteins and provide new insight into the dynamics of horn fly-cattle interactions in nature.
Asunto(s)
Control de Insectos/métodos , Proteínas de Insectos/inmunología , Muscidae/genética , Muscidae/inmunología , Vacunación , Vacunas Sintéticas/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos/sangre , Bovinos , Conducta Alimentaria , Femenino , Proteínas de Insectos/química , Proteínas de Insectos/genética , Datos de Secuencia Molecular , Muscidae/fisiología , Isoformas de Proteínas , Conejos , Glándulas Salivales/metabolismoRESUMEN
Blood-sucking arthropods are vectors responsible for the transmission of several pathogens and parasites to vertebrate animals. The horn fly Haematobia irritans irritans (Diptera: Muscidae) and the tick Boophilus microplus are important hematophagous ectoparasites that cause losses in cattle production. A serine protease inhibitor from a thorax extract of the fly H. irritans irritans (HiTI) was previously isolated, characterized and cloned. In the present study we described the expression, purification, and characterization of the recombinant HiTI (rHiTI) and its possible role in the control of different endogenous and bacterial proteases. rHiTI was successfully expressed using the pPIC9 expression vector with a yield of 4.2 mg/L of active rHiTI. The recombinant HiTI purified by affinity chromatography on trypsin-Sepharose had a molecular mass of 6.53 kDa as determined by LS-ESI mass spectrometry and inhibition constants (Kis) similar to those of native HiTI for bovine trypsin and human neutrophil elastase of 0.4 and 1.0 nM, respectively. Purified rHiTI also showed inhibitory activity against the trypsin-like enzyme of H. i. irritans using its possible natural substrates, fibrinogen and hemoglobin; and also inhibited the OmpT endoprotease of Escherichia coli using fluorogenic substrates. The present results confirm that HiTI may play a role in the control of fly endogenous proteases but also suggest a role in the inhibition of pathogen proteases.
Asunto(s)
Muscidae/química , Inhibidores de Serina Proteinasa/fisiología , Secuencia de Aminoácidos , Animales , Bovinos , Cromatografía de Afinidad , Clonación Molecular , Electroforesis en Gel de Poliacrilamida , Fibrinógeno/metabolismo , Regulación Enzimológica de la Expresión Génica , Hemoglobinas/metabolismo , Elastasa de Leucocito/antagonistas & inhibidores , Datos de Secuencia Molecular , Peso Molecular , Muscidae/enzimología , Muscidae/inmunología , Reacción en Cadena de la Polimerasa , Inhibidores de Serina Proteinasa/química , Inhibidores de Serina Proteinasa/genética , Inhibidores de Serina Proteinasa/aislamiento & purificación , Espectrometría de Masa por Ionización de Electrospray , Tripsina/efectos de los fármacos , Tripsina/metabolismoRESUMEN
To evaluate an immunization procedure using antigens from Haematobia irritans intestine (AgHiI), four bovines (group I) were inoculated with AgHiI mixed with Freund's incomplete adjuvant containing Lactobacillus casei, three bovines (group II) received AgHiI, and three bovines (group III) received saline solution. At day 35, blood was collected from each animal to feed H. irritans flies. There was no difference in the fly mortality observed in the three groups. The percentage of reduction of eggs oviposited by each female in 8 days (%RE), as compared with group III, was 29.45 for group I and 11.02 for group II. Antibody levels (AbL) to AgHiI were higher in group I than in groups II and III. A high correlation between %RE and AbL was observed.
Asunto(s)
Antígenos/inmunología , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/prevención & control , Inmunización/veterinaria , Muscidae/inmunología , Muscidae/patogenicidad , Animales , Formación de Anticuerpos , Bovinos , Femenino , Adyuvante de Freund/administración & dosificación , Lacticaseibacillus casei/inmunología , Lacticaseibacillus casei/fisiologíaRESUMEN
The potential for controlling blood-feeding by the cattle pest, Haematobia irritans irritans (horn fly), was tested by vaccination against thrombostasin (TS), an inhibitor of mammalian thrombin that is released into skin during horn fly blood-feeding. The increase in blood meal size that occurred for flies feeding on sensitized non-vaccinated hosts was blocked and egg development in female flies was delayed when horn flies fed on rabbits and cattle immunized with recombinant TS. This demonstration of the impact of disrupting TS action by vaccination provides a novel approach toward control of this veterinary pest and offers a paradigm for limiting blood-feeding in other medically-important insect species.
Asunto(s)
Control de Insectos/métodos , Proteínas de Insectos/inmunología , Muscidae/inmunología , Muscidae/fisiología , Vacunas Sintéticas/inmunología , Animales , Sangre , Coagulación Sanguínea , Bovinos , Conducta Alimentaria , Inmunoglobulina G/inmunología , Proteínas de Insectos/genética , Muscidae/crecimiento & desarrollo , Conejos , Vacunación , Vacunas Sintéticas/administración & dosificaciónRESUMEN
Several peritrophins of larvae of Lucilia cuprina (sheep blowfly) have demonstrated potential as vaccine antigens, and some have been characterised and cloned. These proteins are tightly associated with the peritrophic matrix, a chitinous tube or sac lining the lumen of the gut of most insects. The peritrophins require strong denaturants for their removal from peritrophic matrix. We now report the preliminary characterisation of peritrophins of the adult stage of L. cuprina and Haematobia irritans exigua (buffalo fly). Similar SDS-PAGE profiles were obtained for proteins extracted in SDS or urea from isolated adult peritrophic matrices of both species. Radioiodination of urea-extracted peritrophins improved sensitivity, indicating numerous proteins of 15-75 kDa. Direct radioiodination of L. cuprina peritrophic matrix preferentially labelled high molecular weight complexes and proteins of 80-90 kDa. Two-dimensional gel analyses of a urea extract of adult L. cuprina peritrophic matrix revealed that most proteins were moderately acidic. Antibodies produced against SDS-extracted peritrophins, or against sonicated peritrophic matrices of these two flies were crossreactive. The sera also appeared to recognise SDS-extracted components of Triton X-100 treated and washed adult peritrophic matrix of the mosquito, Aedes vigilax (Skause). This profile altered as the peritrophic matrix matured. In concordance with extracts from the adult L. cuprina and H.i. exigua peritrophic matrices, proteins in the 50-75 kDa region were immunodominant. The vaccine potential of the peritrophins of these Diptera were examined following vaccination of cattle and rabbits with adult H.i. exigua or L. cuprina peritrophins. When the adult life stages of H.i. exigua or two mosquitoes, A. vigilax and A. aegypti (Linnaeus), were fed on the sera or blood of vaccinated hosts, there were no detrimental effects to any life cycle stages of these Diptera.
Asunto(s)
Dípteros/inmunología , Infestaciones Ectoparasitarias/veterinaria , Proteínas de Insectos/inmunología , Glicoproteínas de Membrana/inmunología , Vacunas/inmunología , Aedes/inmunología , Animales , Anticuerpos/sangre , Bovinos , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/prevención & control , Reacciones Cruzadas , Infestaciones Ectoparasitarias/inmunología , Infestaciones Ectoparasitarias/prevención & control , Muscidae/inmunología , ConejosRESUMEN
Antibody response to feeding by the horn fly, Haematobia irritans irritans (L.), was measured in naturally infested cattle as well as in controlled infestations. Humoral antibody level was not correlated with fly abundance in natural infestations. Correlations between the antibody response and fly abundance were extremely variable among animals. Antibody response to horn fly salivary antigens peaked within 4 wk after fly abundance reached 150 flies per animal. In controlled infestations, antibody response was weak during an initial exposure to 200 flies per animal, but increased after flies were removed from the cattle. Horn fly salivary antigen may have an immunomodulatory effect on the host.
Asunto(s)
Formación de Anticuerpos , Enfermedades de los Bovinos/parasitología , Mordeduras y Picaduras de Insectos/veterinaria , Muscidae/inmunología , Animales , Bovinos , Enfermedades de los Bovinos/inmunología , Femenino , Mordeduras y Picaduras de Insectos/inmunología , Masculino , Glándulas Salivales/inmunologíaRESUMEN
The incorporation of soybean trypsin inhibitor (SBTI) into the diet of the buffalo fly, Haematobia irritans exigua (De Meijere), results in increased mortality and reduced fecundity. A trypsin-like enzyme which binds to SBTI was isolated by affinity chromatography on a Sepharose-SBTI column followed by ion-exchange chromatography. The enzyme was inhibited by benzamidine, phenylmethylsulfonyl fluoride, ovomucoid, leupeptin and alpha-2 macroglobulin. The enzyme was not inhibited by EDTA or p-chloromecuribenzoic acid and had a broad pH optimum of pH 7-9. Vaccination of sheep produced antibodies specific for the trypsin-like enzyme which inhibited enzyme activity in vitro but did not affect the survival of flies maintained in in vitro culture.
Asunto(s)
Endopeptidasas/aislamiento & purificación , Muscidae/enzimología , Animales , Bovinos , Endopeptidasas/química , Concentración de Iones de Hidrógeno , Muscidae/inmunología , Ovinos/inmunología , Tripsina/química , Inhibidores de Tripsina/farmacología , VacunaciónRESUMEN
Pairs of rabbits were immunised with three antigenic preparations derived from Stomoxys calcitrans gut, abdominal section and whole flies. Immunoblotting studies demonstrated that a humoral response was mounted against eight antigens from the gut preparation and 12 each from the abdominal and whole fly preparations. In vitro feeding experiments showed higher mortality between Days 4 and 7 in the group of flies which had fed upon blood from rabbits inoculated with the gut derived antigen. This group also produced the lowest percentage of viable eggs (15.5%).
Asunto(s)
Animales Domésticos/parasitología , Antígenos/inmunología , Infestaciones Ectoparasitarias/veterinaria , Muscidae/inmunología , Vacunación/veterinaria , Animales , Formación de Anticuerpos , Infestaciones Ectoparasitarias/prevención & control , Femenino , Immunoblotting , Masculino , ConejosRESUMEN
Naturally acquired immunity to buffalo fly (Haematobia irritans exigua) infestation was examined in cattle. Animals exposed to flies had serum antibodies to buffalo fly antigens at levels that correlated with the intensity of exposure. Two weeks of intense exposure to buffalo fly induced an increase in peripheral blood eosinophil numbers and a concomitant rise in serum antibody levels in exposed animals. Antigens specific for antibody induced by natural exposure were identified using antisera from exposed cattle to probe Western blots of whole fly homogenate separated using SDS-PAGE. Similar immunoreactive bands were found with buffalo fly saliva. Immunoreactive proteins were partially purified from whole fly homogenates by anion-exchange chromatography. Fractions eluted from columns were screened using Western blots probed with serum from exposed animals. Exposed animals showed immediate hypersensitivity to partially purified antigens and to buffalo fly saliva. Flies which fed on exposed animals with high serum levels of antibody to fly antigens did not show greater mortality than flies fed on unexposed animals.