RESUMEN

Bioprinting is an emerging tissue engineering technique that has attracted the attention of researchers around the world, for its ability to create tissue constructs that recapitulate physiological function. While the technique has been receiving hype, there are still limitations to the use of bioprinting in practical applications, much of which is due to inappropriate bioink design that is unable to recapitulate complex tissue architecture. Silk fibroin (SF) is an exciting and promising bioink candidate that has been increasingly popular in bioprinting applications because of its processability, biodegradability, and biocompatibility properties. However, due to its lack of optimum gelation properties, functionalization strategies need to be employed so that SF can be effectively used in bioprinting applications. These functionalization strategies are processing methods which allow SF to be compatible with specific bioprinting techniques. Previous literature reviews of SF as a bioink mainly focus on discussing different methods to functionalize SF as a bioink, while a comprehensive review on categorizing SF functional methods according to their potential applications is missing. This paper seeks to discuss and compartmentalize the different strategies used to functionalize SF for bioprinting and categorize the strategies for each bioprinting method (namely, inkjet, extrusion, and light-based bioprinting). By compartmentalizing the various strategies for each printing method, the paper illustrates how each strategy is better suited for a target tissue application. The paper will also discuss applications of SF bioinks in regenerating various tissue types and the challenges and future trends that SF can take in its role as a bioink material.

Asunto(s)

Bioimpresión/instrumentación , Bombyx/metabolismo , Fibroínas/fisiología , Animales , Bioimpresión/métodos , Fibroínas/biosíntesis , Impresión Tridimensional , Ingeniería de Tejidos/métodos , Andamios del Tejido

RESUMEN

Fibroin of the silkworm consists of fibroin heavy chain (Fib-H) with hydrophobic intermediate repeats flanked by hydrophilic N and C terminal domains (NTD and CTD, respectively), fibroin light chain (Fib-L), and P25. However, the respective roles of each polypeptide in silk processing remain largely unknown. Here, a series of transgenic silkworms with different fusion gene expression cassettes were created in order to selectively express different fluorescent fusion proteins in silk glands. The roles of different components in silk processing were investigated via observing and analyzing the movement and distribution of these proteins in the silk gland and in cocoon silk. The data showed that hydrophilic NTDs were distributed on the surface of micelles, providing sufficient electrostatic repulsion to prevent premature crystallization of silk proteins. Hydrophilic CTD==Ls ("==" represents the disulfide bond) were located on the inner layer of micelles to control the solubility of large micelles. The results presented here elucidated the underlying mechanisms of silkworm silk processing in vivo. This is significant for the development of artificial spinning technology, novel silk biomaterials, and silk gland expression systems.

Asunto(s)

Bombyx/metabolismo , Fibroínas/química , Fibroínas/metabolismo , Animales , Animales Modificados Genéticamente/genética , Materiales Biocompatibles/metabolismo , Secreciones Corporales/metabolismo , Bombyx/química , Fibroínas/fisiología , Proteínas de Insectos/genética , Dominios Proteicos/fisiología , Seda/metabolismo

RESUMEN

Silk fibroin is a novel biomaterial for enhancing transplanted islet cell function and survival. This study investigated whether silk fibroin may have unique properties that improve islet function in the face of inflammatory-mediated stress during transplantation. Murine islet function was tested in vitro with either silk fibroin or alginate and challenged with inflammatory cytokines. The glucose-stimulated insulin secretion index for all conditions decreased with inflammatory cytokines, but was better preserved for islets exposed to silk compared to those exposed to alginate or medium. GLUT2 transporter expression on the cell surface of islets exposed to silk was increased compared to alginate or medium alone. Upon cytokine stress, a greater percentage of islet cells exposed to silk expressed GLUT2 on their surface. We conclude that preconditioning islets with silk fibroin stimulates islet cell surface GLUT2 expression, an increase, which persists under inflammatory stress, and may improve islet engraftment and function after transplantation.

Asunto(s)

Fibroínas/metabolismo , Fibroínas/farmacología , Islotes Pancreáticos/efectos de los fármacos , Alginatos/farmacología , Animales , Fibroínas/fisiología , Transportador de Glucosa de Tipo 2/genética , Transportador de Glucosa de Tipo 2/metabolismo , Inflamación , Células Secretoras de Insulina/efectos de los fármacos , Islotes Pancreáticos/fisiología , Trasplante de Islotes Pancreáticos/métodos , Trasplante de Islotes Pancreáticos/fisiología , Masculino , Ratones , Ratones Endogámicos C57BL , Seda/fisiología , Estrés Fisiológico/efectos de los fármacos

RESUMEN

AIM: This in vitro and in vivo study reports on silk fibroin (SF) scaffold, functionalized for in situ delivery of GABA and/or allopregnanolone (ALLO), as biomaterial for potential application in tissue engineering and nerve regeneration. MATERIALS & METHODS: We evaluated the feasibility to design 2D scaffolds (films) made of regenerated Bombyx mori SF, functionalized with GABA and/or ALLO to enhance in vitro biological functions, health, survival and growth of Schwann cells and sensitive neurons of the dorsal root ganglia. RESULTS & CONCLUSION: Our 2D-SF film showed an efficient loading and controllable release of drugs promoting nerve regeneration. SF functionalized film may be helpful for the development of bioengineered conduits and, in principle, have great potential for long-gap nerve injury repair.

Asunto(s)

Fibroínas/fisiología , Neuronas/citología , Células de Schwann/citología , Seda , Ingeniería de Tejidos/métodos , Animales , Materiales Biocompatibles , Bombyx , Fibroínas/química , Ensayo de Materiales , Regeneración Nerviosa , Pregnanolona/química , Pregnanolona/fisiología , Ratas , Ratas Sprague-Dawley , Ácido gamma-Aminobutírico/química , Ácido gamma-Aminobutírico/fisiología

RESUMEN

Biocomposite matrices with high mechanical strength, high stability, and the ability to direct matrix-specific stem cell differentiation are essential for the reconstruction of lesioned tissues in tissue engineering and cell therapeutics. Toward this end, we used the electrospinning technique to fabricate well-aligned composite fibers from collagen and spider dragline silk protein, obtained from the milk of transgenic goats, mimicking the native extracellular matrix (ECM) on a similar scale. Collagen and the dragline silk proteins were found to mix homogeneously at all ratios in the electrospun (E-spun) fibers. As a result, the ultimate tensile strength and elasticity of the fibers increased monotonically with silk percentage, whereas the stretchability was slightly reduced. Strikingly, we found that the incorporation of silk proteins to collagen dramatically increased the matrix stability against excessive fiber swelling and shape deformation in cell culture medium. When human decidua parietalis placental stem cells (hdpPSCs) were seeded on the collagen-silk matrices, the matrices were found to support cell proliferation at a similar rate as that of the pure collagen matrix, but they provided cell adhesion with reduced strengths and induced cell polarization at varied levels. Matrices containing 15 and 30 wt % silk in collagen (CS15, CS30) were found to induce a level of neural differentiation comparable to that of pure collagen. In particular, CS15 matrix induced the highest extent of cell polarization and promoted the development of extended 1D neural filaments strictly in-line with the aligned fibers. Taking the increased mechanical strength and fiber stability into consideration, CS15 and CS30 E-spun fibers offer better alternatives to pure collagen fibers as scaffolds that can be potentially utilized in neural tissue repair and the development of future nanobiodevices.

Asunto(s)

Células Madre Adultas/fisiología , Materiales Biocompatibles , Diferenciación Celular/fisiología , Colágeno Tipo I/fisiología , Colágeno/fisiología , Fibroínas/fisiología , Células Madre Adultas/efectos de los fármacos , Animales , Materiales Biocompatibles/administración & dosificación , Materiales Biocompatibles/química , Fenómenos Biomecánicos/fisiología , Bovinos , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Colágeno/administración & dosificación , Colágeno/química , Colágeno Tipo I/administración & dosificación , Colágeno Tipo I/química , Femenino , Fibroínas/administración & dosificación , Fibroínas/química , Humanos , Placenta/citología , Embarazo , Seda/administración & dosificación , Seda/química , Seda/fisiología , Ingeniería de Tejidos/métodos

RESUMEN

We investigated the natural variation in silk composition and mechanical performance of the orb-weaving spider Argiope trifasciata at multiple spatial and temporal scales in order to assess how protein composition contributes to the remarkable material properties of spider dragline silk. Major ampullate silk in orb-weaving spiders consists predominantly of two proteins (MaSp1 and MaSp2) with divergent amino acid compositions and functionally different microstructures. Adjusting the expression of these two proteins therefore provides spiders with a simple mechanism to alter the material properties of their silk. We first assessed the reliability and precision of the Waters AccQ-Tag amino acid composition analysis kit for determining the amino acid composition of small quantities of spider silk. We then tested how protein composition varied within single draglines, across draglines spun by the same spider on different days, and finally between spiders. Then, we correlated chemical composition with the material properties of dragline silk. Overall, we found that the chemical composition of major ampullate silk was in general homogeneous among individuals of the same population. Variation in chemical composition was not detectable within silk spun by a single spider on a single day. However, we found that variation within a single spider's silk across different days could, in rare instances, be greater than variation among individual spiders. Most of the variation in silk composition in our investigation resulted from a small number of outliers (three out of sixteen individuals) with a recent history of stress, suggesting stress affects silk production process in orb web spiders. Based on reported sequences for MaSp genes, we developed a gene expression model showing the covariation of the most abundant amino acids in major ampullate silk. Our gene expression model supports that dragline silk composition was mostly determined by the relative abundance of MaSp1 and MaSp2. Finally, we showed that silk composition (especially proline content) strongly correlated with some measures of mechanical performance, particularly how much fibers shrunk during supercontraction as well as their breaking strains. Our findings suggest that spiders are able to change the relative expression rates of different MaSp genes to produce silk fibers with different chemical compositions, and hence, different material properties.

Asunto(s)

Fibroínas/química , Fibroínas/fisiología , Seda/química , Seda/fisiología , Resistencia a la Tracción/fisiología , Animales , Fenómenos Biomecánicos/fisiología , Femenino , Arañas

RESUMEN

Novel hybrid functional materials are formed by combining hydrotalcite-like compounds and silk fibroin (SF-HTlc) via an environmental friendly aqueous process. The nanocomposites can be prepared with different weight ratio of the constituting components and preserve the conformational properties of the silk protein and the lamellar structure of hydrotalcites. Optical microscopy, scanning electron microscopy, and atomic force microscopy analyses show a good dispersion degree of the inorganic nanoparticles into the organic silk matrix. A mutual benefit on the stability of both organic and inorganic components was observed in the nanocomposites. SF-HTlc displayed limited dissolution of hydrotalcite in acidic medium, enhanced mechanical properties, and higher protease resistance of silk protein. The transparency, flexibility, and acidic environment resistance of silk fibroin combined to the protective and reinforcing properties of hydrotalcites generate a hybrid material, which is very attractive for applications in recently reported silk based opto-electronic and photonics technologies.

Asunto(s)

Hidróxido de Aluminio/química , Fibroínas/química , Hidróxido de Magnesio/química , Nanocompuestos/química , Seda/química , Animales , Bombyx , Sinergismo Farmacológico , Fibroínas/fisiología , Seda/fisiología

RESUMEN

We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.

Asunto(s)

Materiales Biocompatibles/química , Bombyx , Técnicas de Cultivo de Célula/métodos , Fibroínas/química , Animales , Materiales Biocompatibles/farmacología , Adhesión Celular/efectos de los fármacos , Línea Celular , Fibroínas/farmacología , Fibroínas/fisiología , Humanos , Esterilización , Propiedades de Superficie , Resistencia a la Tracción/fisiología , Ingeniería de Tejidos

RESUMEN

Wound healing is a biological process directed to the restoration of tissue that has suffered an injury. An important phase of wound healing is the generation of a basal epithelium able to wholly replace the epidermis of the wound. A broad range of products derived from fibroin and sericin from Bombyx mori silk are used to stimulate wound healing. However, so far the molecular mechanism underlying this phenomenon has not been elucidated. The aim of this work was to determine the molecular basis underlying wound healing properties of silk proteins using a cell model. For this purpose, we assayed fibroin and sericin in a wound healing scratch assay using MDA-MB-231 and Mv1Lu cells. Both proteins stimulated cell migration. Furthermore, treatment with sericin and fibroin involved key factors of the wound healing process such as upregulation of c-Jun and c-Jun protein phosphorylation. Moreover, fibroin and sericin stimulated the phosphorylation of ERK 1/2 and JNK 1/2 kinases. All these experiments were done in the presence of specific inhibitors for some of the cell signalling pathways referred above. The obtained results revealed that MEK, JNK and PI3K pathways are involved in fibroin and sericin stimulated cells migration. Inhibition of these three kinases prevented c-Jun upregulation and phosphorylation by fibroin or sericin. Fibroin and sericin were tested in the human keratinocyte cell line, HaCaT, with similar results. Altogether, our results showed that fibroin and sericin initiate cell migration by activating the MEK, JNK and PI3K signalling pathways ending in c-Jun activation.

Asunto(s)

Movimiento Celular/fisiología , Fibroínas/fisiología , Proteínas Proto-Oncogénicas c-jun/metabolismo , Sericinas/fisiología , Seda/química , Regulación hacia Arriba/fisiología , Animales , Bombyx , Línea Celular , Humanos , Fosforilación , Inhibidores de Proteínas Quinasas/farmacología , Proteínas Quinasas/metabolismo

RESUMEN

Spider silk is a self-assembling biopolymer that outperforms many known materials in terms of its mechanical performance despite being constructed from simple and inferior building blocks. While experimental studies have shown that the molecular structure of silk has a direct influence on the stiffness, toughness, and failure strength of silk, few molecular-level analyses of the nanostructure of silk assemblies in particular under variations of genetic sequences have been reported. Here we report atomistic-level structures of the MaSp1 protein from the Nephila Clavipes spider dragline silk sequence, obtained using an in silico approach based on replica exchange molecular dynamics (REMD) and explicit water molecular dynamics. We apply this method to study the effects of a systematic variation of the poly-alanine repeat lengths, a parameter controlled by the genetic makeup of silk, on the resulting molecular structure of silk at the nanoscale. Confirming earlier experimental and computational work, a structural analysis reveals that poly-alanine regions in silk predominantly form distinct and orderly ß-sheet crystal domains while disorderly regions are formed by glycine-rich repeats that consist of 3(10)-helix type structures and ß-turns. Our predictions are directly validated against experimental data based on dihedral angle pair calculations presented in Ramachandran plots combined with an analysis of the secondary structure content. The key result of our study is our finding of a strong dependence of the resulting silk nanostructure depending on the poly-alanine length. We observe that the wildtype poly-alanine repeat length of six residues defines a critical minimum length that consistently results in clearly defined ß-sheet nanocrystals. For poly-alanine lengths below six, the ß-sheet nanocrystals are not well-defined or not visible at all, while for poly-alanine lengths at and above six, the characteristic nanocomposite structure of silk emerges with no significant improvement of the quality of the ß-sheet nanocrystal geometry. We present a simple biophysical model that explains these computational observations based on the mechanistic insight gained from the molecular simulations. Our findings set the stage for understanding how variations in the spidroin sequence can be used to engineer the structure and thereby functional properties of this biological superfiber, and present a design strategy for the genetic optimization of spidroins for enhanced mechanical properties. The approach used here may also find application in the design of other self-assembled molecular structures and fibers and in particular biologically inspired or completely synthetic systems.

Asunto(s)

Proteínas de Insectos/química , Seda/química , Arañas , Animales , Fibroínas/química , Fibroínas/fisiología , Proteínas de Insectos/fisiología , Modelos Moleculares , Estructura Molecular , Nanoestructuras/química , Seda/fisiología

RESUMEN

Fibrous proteins in nature fulfill a wide variety of functions in different structures ranging from cellular scaffolds to very resilient structures like tendons and even extra-corporal fibers such as silks in spider webs or silkworm cocoons. Despite their different origins and sequence varieties many of these fibrous proteins share a common building principle: they consist of a large repetitive core domain flanked by relatively small non-repetitive terminal domains. Amongst protein fibers, spider dragline silk shows prominent mechanical properties that exceed those of man-made fibers like Kevlar. Spider silk fibers assemble in a spinning process allowing the transformation from an aqueous solution into a solid fiber within milliseconds. Here, we highlight the role of the non-repetitive terminal domains of spider dragline silk proteins during storage in the gland and initiation of the fiber assembly process.

Asunto(s)

Fibroínas/química , Arañas/fisiología , Secuencia de Aminoácidos , Animales , Bombyx/fisiología , Fibroínas/fisiología , Concentración de Iones de Hidrógeno , Micelas , Modelos Moleculares , Datos de Secuencia Molecular , Estructura Terciaria de Proteína , Alineación de Secuencia , Homología de Secuencia de Aminoácido

RESUMEN

Spider dragline silk is an outstanding material made up of unique proteins-spidroins. Analysis of the amino acid sequences of full-length spidroins reveals a tripartite composition: an N-terminal non-repetitive domain, a highly repetitive central part composed of approximately 100 polyalanine/glycine rich co-segments and a C-terminal non-repetitive domain. Recent molecular data on the terminal domains suggest that these have different functions. The composite nature of spidroins allows for recombinant production of individual and combined regions. Miniaturized spidroins designed by linking the terminal domains with a limited number of repetitive segments recapitulate the properties of native spidroins to a surprisingly large extent, provided that they are produced and isolated in a manner that retains water solubility until fibre formation is triggered. Biocompatibility studies in cell culture or in vivo of native and recombinant spider silk indicate that they are surprisingly well tolerated, suggesting that recombinant spider silk has potential for biomedical applications.

Asunto(s)

Materiales Biocompatibles , Fibroínas , Arañas , Secuencia de Aminoácidos , Animales , Fibroínas/química , Fibroínas/genética , Fibroínas/fisiología , Humanos , Datos de Secuencia Molecular , Plantas Modificadas Genéticamente , Estructura Terciaria de Proteína , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/fisiología , Medicina Regenerativa , Análisis de Secuencia , Relación Estructura-Actividad

RESUMEN

The spirally arrayed viscous capture threads of spider orb-webs are formed of small, regularly spaced adhesive droplets and are responsible for retaining insects that strike the web, giving a spider more time to subdue these prey. These sticky threads are deposited from the perimeter of the web inward. We tested the hypothesis that depletion of silk reserves during web construction affects the properties of capture threads spun by adult female Argiope aurantia and Argiope trifasciata. In both species the droplet volume (DV) per millimeter thread length was the same in outer and inner capture threads and in early and late season webs. However, the outer threads of both species were stickier than their inner threads and, consequently, had a greater stickiness per DV. Thus, dwindling silk reserves during web construction appeared to reduce the stickiness of both species' threads by changing the composition rather than the volume of their viscous droplets. In A. trifasciata, which we studied later in the fall than A. aurantia, there were also seasonal declines in both thread stickiness and stickiness per DV, which may result from either the depletion of silk reserves or the reallocation of these resources. Early season webs of A. trifasciata also had greater stickiness per square centimeter of capture area than late season webs, better equipping these early webs to retain insects.

Asunto(s)

Fibroínas/fisiología , Arañas/fisiología , Animales , Femenino , Fibroínas/metabolismo , Conducta Predatoria/fisiología , Estaciones del Año , Arañas/metabolismo , Viscosidad

RESUMEN

Spiders spin high-performance silks through the expression and assembly of tissue-restricted fibroin proteins. Spider silks are composite protein biopolymers that have complex microstructures. Retrieval of cDNAs and genomic DNAs encoding silk fibroins has revealed an association between the protein sequences and structure-property relationships. However, before spider silks can be subject to genetic engineering for commercial applications, the complete protein sequences and their functions, as well as the details of the spinning mechanism, will require additional progress and collaborative efforts in the areas of biochemistry, molecular biology and material science. Novel approaches to reveal additional molecular constituents embedded in the spider fibers, as well as cloning strategies to manipulate the genes for expression, will continue to be important aspects of spider biology research. Here we summarize the molecular characteristics of the different spider fibroins, the mechanical properties and assembly process of spidroins and the advances in protein expression systems used for recombinant silk production. We also highlight different technical approaches being used to elucidate the molecular constituents of silk fibers.

Asunto(s)

Fibroínas/biosíntesis , Fibroínas/genética , Proteínas de Insectos/biosíntesis , Seda/química , Arañas/química , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Animales , Fibroínas/química , Fibroínas/fisiología , Proteínas de Insectos/genética , Datos de Secuencia Molecular , Proteínas Recombinantes , Secuencias Repetitivas de Aminoácido , Homología de Secuencia de Aminoácido , Seda/fisiología , Seda/ultraestructura

RESUMEN

The regenerated liquid silk fibroin with an average molecular mass of about 60 kDa consists of 18 kinds of amino acids containing approximately 10% of polar amino acids with hydroxyl and amino groups such as serine and lysine. The liquid silk fibroin is coupled covalently with insulin molecules through these strongly polar side groups by using glutaraldehyde. The physicochemical properties of the silk fibroin-insulin (SF-Ins) bioconjugates were investigated by enzyme-linked immunosorbent assay for the quantitative measurement of insulin. The biological activities of the insulin bioconjugates were characterized in vitro and in vivo. The SF-Ins constructs obtained by 5 h of covalent crosslinking showed much higher recovery (about 70%) and in vitro stability in human serum than bovine serum albumin-insulin (BSA-Ins) derivatives. The results in human serum indicated that the half-life in vitro of the biosynthesized SF-Ins derivatives was 2.1 and 1.7 times more than that of BSA-Ins conjugates and native insulin, respectively. The immunogenicity of the regenerated silk fibroin and the antigenicity of silk fibroin-modified insulin were not observed in both rabbits and rats. The pharmacological activity of the SF-Ins bioconjugates in diabetic rats evidently lengthened and was about 3.5 times as long as that of the native insulin, nearly 21 h. The bioconjugation of insulin with the regenerated silk fibroin greatly improved its physicochemical and biological stability.

Asunto(s)

Fibroínas/biosíntesis , Insulina/biosíntesis , Animales , Bombyx , Reactivos de Enlaces Cruzados , Fibroínas/química , Fibroínas/inmunología , Fibroínas/fisiología , Insulina/química , Insulina/inmunología , Insulina/fisiología , Masculino , Conejos , Ratas

RESUMEN

Unlike man-made fibers, the silks of spiders are spun from aqueous solutions and at atmospheric pressure in a process still poorly understood. The molecular mechanism of this process involves the conversion of a highly concentrated, predominantly disordered silk protein (spidroin) into beta-sheet-rich structures. To help store and transport the spidroins in solution, as well as probably control their conversion, a liquid crystalline arrangement is established in the storage region in the ampulla and persists into the duct. Although it has been suggested that changes in the concentration of hydrogen and metal ions play a role in the formation of the solid thread, there is no reported evidence that these ions influence the secondary structure of native spidroin in solution. Here, we demonstrate that pH values between approximately 3.5 and 4.5 induce a slow change of conformation from the disordered to the beta-sheet-rich form. We also report that Al(3+), K(+), and Na(+) ions induce similar changes in structure, while Ca(2+) and Mg(2+) stabilize the predominantly disorder state of the protein. Cs(+) and Li(+) have no apparent effect. Direct volumetric and spectrophotometric titration showed a pI of 4.22 +/- 0.33 and apparent pK values of 6.74 +/- 0.71 and 9.21 +/- 0.27, suggesting a mechanism for the effect of low pH on the protein and a rationale for the observed reduction in pH in the duct. We discuss the importance of these findings for the spinning process and the active role played by the spider to alter the kinetics of the transition.

Asunto(s)

Cationes Bivalentes/química , Cationes Monovalentes/química , Fibroínas/química , Arañas/química , Animales , Cationes Monovalentes/metabolismo , Dicroismo Circular , Femenino , Fibroínas/metabolismo , Fibroínas/fisiología , Concentración de Iones de Hidrógeno , Cinética , Transición de Fase , Conformación Proteica , Estructura Secundaria de Proteína , Soluciones , Espectrofotometría Ultravioleta , Arañas/metabolismo , Arañas/fisiología , Volumetría

RESUMEN

Silk fibroin of Bombyx mori is secreted from the posterior silk gland (PSG) as a 2.3-MDa elementary unit, consisting of six sets of a disulfide-linked heavy chain (H-chain)-light chain (L-chain) heterodimer and one molecule of fibrohexamerin (fhx)/P25. Fhx/P25, a glycoprotein, associates noncovalently with the H-L heterodimers. The elementary unit was found and purified from the endoplasmic reticulum (ER) extract of PSG cells. A substantial amount of fhx/P25 unassembled into the elementary unit was also present in ER. In normal-level fibroin-producing breeds (J-139 and C108), the elementary unit contained fhx/P25 of either 30 kDa (major) or 27 kDa (minor). The 27-kDa fhx/P25 was produced from the 30-kDa form by digestion with the bacterial alpha1,2-mannosidase in vitro. The elementary unit in the ER extract contained only the 30-kDa fhx/P25, whereas both 30- and 27-kDa forms of fhx/P25 were present in the ER plus Golgi mixed extracts. In naked-pupa mutants [Nd(2), Nd-s and Nd-sD], extremely small amounts of fibroin were produced and they consisted of one molecule of 27-kDa fhx/P25 and six molecules of H-chain but no L-chain. When the Nd-sD mutant was subjected to transgenesis with the normal L-chain gene, the (H-L)6fhx1-type elementary unit containing the 30-kDa fhx/P25, was produced. These results suggest that fhx/P25 in the elementary unit is largely protected from digestion with Golgi alpha1,2-mannosidases when L-chains are present in the unit. Models suggesting a role of L-chain for the protection of alpha1,2-mannose residues of fhx/P25 are presented.

Asunto(s)

Retículo Endoplásmico/metabolismo , Fibroínas/metabolismo , Fibroínas/fisiología , Glicoproteínas/metabolismo , Proteínas de Insectos/metabolismo , Proteínas de Insectos/fisiología , Manosidasas/metabolismo , Animales , Animales Modificados Genéticamente , Western Blotting , Bombyx/fisiología , Ensayo de Inmunoadsorción Enzimática , Aparato de Golgi/enzimología , Larva/genética , Larva/metabolismo , Manosa/metabolismo , Pupa/genética , Pupa/metabolismo , Seda , Transformación Genética , Transgenes

RESUMEN

The mechanical properties of regenerated cartilage tissue were measured to evaluate changes in their visco-elastic properties during cultivation. An indentation test and dynamic visco-elasticity measurements were carried out on cartilage tissue cultured with rabbit chondrocytes that had been inoculated into the fibroin sponge. A 1.5-mm-diameter porous indentor was used for the indentation test, in which time-dependent strain curves were derived from measurements taken under several loading conditions. Dynamic visco-elasticity measurements were performed under compressive loading to evaluate the load-bearing function of the articular cartilage. Although the amount of permanent deformation was not influenced by the duration of cartilage regeneration, the amount of creep deformation increased with longer cultivation. The E' value of the regenerated cartilage increased and the peak value of tan delta and the frequency at the peak became lower with longer cultivation. It is suggested that the changes in the time-dependent strain curves and dynamic visco-elastic properties of the regenerated cartilage were caused by maturation of the cultured cartilage tissue.

Asunto(s)

Cartílago Articular/fisiología , Condrocitos/fisiología , Condrogénesis/fisiología , Fibroínas/fisiología , Regeneración/fisiología , Soporte de Peso/fisiología , Animales , Técnicas de Cultivo/métodos , Elasticidad , Fémur/fisiología , Pruebas de Dureza/métodos , Húmero/fisiología , Conejos , Valores de Referencia , Sensibilidad y Especificidad , Especificidad de la Especie , Porcinos , Tibia/fisiología , Recolección de Tejidos y Órganos/métodos , Viscosidad

RESUMEN

Components of desmosomes, filaments, and keratohyaline granules were studied by electron microscope and biochemical methods to clarify their role in the stabilization and keratinization of the epidermis. Isolated desmosomes are composed of 76% protein, 17% carbohydrate, and 10% lipid. The bulk of protein consists of a "spectrin"-like fibrous protein, presumably present in the plaque, and of glycoproteins in the desmosomal interspace. The main component of filaments, prekeratin, is a low-sulfur alpha-protein composed of a pair of three-chain subunits with non-alpha-helical segments separated by 200 A-long alpha-helical regions. The major component of isolated keratohyaline granules, the amorphous particulate material, is formed by a high-sulfur protein with a single-type of polypeptide chain. Polypeptide chains comparable to those found in prekeratin and keratohyaline granules were recovered from extracts of horny cells. Within the living part of the epidermis, filaments hypothetically form a cytoskeletal system which is anchored to desmosomes by a filamentous plaque protein. Glycoproteins are involved in the formation of strong junctions between the cells which enable the living part of the epidermis to respond as a whole to mechanical stress. The stratum corneum is stabilized by a similar system in a consolidated state which is less extensible. Horny cells are enveloped by a thickened membrane and the interfilamentous spaces are filled with various proteins including the sulfur-rich amorphous protein found in keratohyaline granules.

Asunto(s)

Desmosomas/análisis , Fibroínas/análisis , Hialina/análisis , Queratinas/biosíntesis , Fenómenos Fisiológicos de la Piel , Animales , Animales Recién Nacidos , Bovinos , Adhesión Celular , Agregación Celular , Membrana Celular/ultraestructura , Desmosomas/fisiología , Desmosomas/ultraestructura , Electroforesis en Gel de Poliacrilamida , Fibroínas/fisiología , Glicoproteínas/análisis , Hialina/fisiología , Uniones Intercelulares/análisis , Microscopía Electrónica , Conformación Proteica , Proteínas/análisis , Ratas , Piel/análisis , Piel/ultraestructura