Your browser doesn't support javascript.
loading
Effect of the sterilization method on the properties of Bombyx mori silk fibroin films.
George, Karina A; Shadforth, Audra M A; Chirila, Traian V; Laurent, Matthieu J; Stephenson, Sally-Anne; Edwards, Grant A; Madden, Peter W; Hutmacher, Dietmar W; Harkin, Damien G.
Afiliación
  • George KA; Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.
  • Shadforth AM; Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia.
  • Chirila TV; Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia; Faculty o
  • Laurent MJ; Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Ecole Supérieure d'Ingénieurs de Luminy (ESIL), Université de la Méditerranée Aix-Marseille II, Luminy case 925 13288, Marseille, Cedex 09, France.
  • Stephenson SA; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
  • Edwards GA; Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia.
  • Madden PW; Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia.
  • Hutmacher DW; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Built Environment and Engineering, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
  • Harkin DG; Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.
Mater Sci Eng C Mater Biol Appl ; 33(2): 668-74, 2013 Mar 01.
Article en En | MEDLINE | ID: mdl-25427472
We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bombyx / Materiales Biocompatibles / Técnicas de Cultivo de Célula / Fibroínas Límite: Animals / Humans Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2013 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bombyx / Materiales Biocompatibles / Técnicas de Cultivo de Célula / Fibroínas Límite: Animals / Humans Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2013 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos