RESUMEN

Calcium (Ca) is an essential mineral for eggshell formation and muscle contraction, and a lack of it can result in poor egg quality and decreased egg output in laying ducks. This study aims to see how feeding the mineral Ca in a ration containing Shrimp head meal and Bilis fish head affects the laying performance and quality of hatching eggs in Mojosari ducks. A total of 105 female and 15 male Mojosari ducks, aged 78 weeks, were raised for three months and randomly divided into 15 flocks (each flock containing seven females and one male duck). There were three kinds of treatment, namely P0 (control, without mineral), P1 (ratio of Shrimp head meal and Bilis fish head 2:1 + 1% mineral), and P2 ((ratio of Shrimp head meal and Bilis fish head 1:2 + 1% mineral). The findings revealed that adding mineral Ca to feed, including Shrimp head meal and Bilis fish head, had no significant influence (P>0.05) on laying Mojosari duck performance in terms of feed intake, egg production, egg weight, egg quality, ducklings produced, and income over feed cost (IOFC). Furthermore, Ca addition in the ration did not result in substantial increases (P>0.05) in fertility, hatchability, or egg size characteristics. Based on the findings of this study, feeding ducks with shrimp head meal and Bilis fish head can be used as an alternate calcium-free feed formulation.

Asunto(s)

Alimentación Animal , Calcio , Patos , Animales , Patos/fisiología , Femenino , Alimentación Animal/análisis , Masculino , Calcio/análisis , Oviposición/efectos de los fármacos , Oviposición/fisiología , Óvulo/efectos de los fármacos , Óvulo/fisiología , Distribución Aleatoria

RESUMEN

The papaya (Carica papaya L.) is among the mainly fruit species produced in tropical and subtropical climate. The salinity of water in agricultural areas is considered a limiting factor for the expansion of papaya. This study aimed to evaluate calcium-enriched microalgae extract (EMa-Ca) as an attenuator of saline stress in irrigation water on the growth and physiology of Formosa papaya seedlings, hybrid Tainung. The experiment was conducted in a protected environment, with treatments distributed in a 5 × 2 factorial scheme, comprising five electrical conductivities of irrigation water (0.50; 1.10; 2.50; 3.90 and 4.50 dSm-1) with the presence and absence of EMa-Ca in the substrate. Evaluated characteristics were: plant height, number of leaves, stem diameter, leaf area, dry masses weight of roots, aboveground parts and total. Gas exchanges and chlorophyll indices (a, b and total) were also evaluated. The application of EMa-Ca resulted in an increase of 6.05% in height and 6.33% in trunk diameter. The number of leaves decreased with an increase in electrical conductivity, and the leaf area was reduced by 33%. All seedling dry masses showed greater declines in the absence of EM-Ca. The EMa-Ca increased net photosynthesis, CO2 concentration, transpiration and stomatal conductance by 39.13%, 30.43%, 38.88% and 42.85%, respectively. For chlorophyll without the use of EMa-Ca, a decrease rate of 1.21%, 0.41% and 1.62% was observed for Chla, Chlb and Chlt, respectively. Therefore, the EMa-Ca application (1.0 ml/L) significantly enhance the vegetative development, gas exchanges, and chlorophyll indices of papaya seedlings under saline stress conditions.

Asunto(s)

Calcio , Carica , Microalgas , Plantones , Carica/química , Carica/efectos de los fármacos , Plantones/efectos de los fármacos , Calcio/análisis , Microalgas/efectos de los fármacos , Microalgas/fisiología , Clorofila/análisis , Estrés Salino/efectos de los fármacos , Fotosíntesis/efectos de los fármacos , Salinidad

RESUMEN

Background: Calcium intake is below recommendations in several parts of the world. Improving calcium intake has benefits not only for bone health but also helps to prevent pregnancy hypertension disorders. Calcium concentration of tap water is usually low The aim of the present study was to determine the maximum amount of calcium that can be added to tap water while complying with drinking water Argentine regulations. Methods: Tap water samples were collected from the Province of Buenos Aires (Argentina). Physicochemical properties and saturation index were measured. Different incremental concentrations of calcium chloride were added to the experimental aliquots. Results: Baseline water had a mean calcium concentration of 22.00 ± 2.54 mg/L, water hardness of 89.9 ± 6.4 mg/L CaCO 3, and a saturation index of -1.50 ± 0.11. After the addition of 0.4554 ± 0.0071 g of salt, water hard-ness reached 355.0 ± 7.1 mg/L CaCO 3, a calcium concentration of 140.50 ± 2.12 mg/L, and a saturation index -0.53 ± 0.02. Conclusions: This study shows that at laboratory level it is feasible to increase calcium concentration of drinking water by adding calcium chloride while complying with national standards. Calcium concentration of drinking tap water could be evaluated and minimum calcium concentration of tap water regulated so as to improve calcium intake in populations with low calcium intake.

Asunto(s)

Calcio , Agua Potable , Agua Potable/química , Agua Potable/normas , Humanos , Argentina , Calcio/análisis , Estudios de Factibilidad , Calcio de la Dieta/análisis , Calidad del Agua/normas , Abastecimiento de Agua/normas , Femenino

RESUMEN

Accurately predicting phosphorous (P) and calcium (Ca) dietary requirements is critical for optimizing dairy cattle performance, and minimizing mineral excretions and ecosystems eutrophication. This study provides a new factorial system to determine net and dietary P and Ca requirements for maintenance and lactation, derived from a meta-regression of mineral trials involving lactating dairy cows. A comprehensive global database was constructed from 57 peer-reviewed articles of mineral balance trials, with a wide range of dietary and animal performance data. We estimated the net requirements for maintenance from the intercept of a nonlinear equation between mineral intake and the sum of total fecal and urinary excretions, which is an estimate of endogenous mineral loss. Mineral secreted in milk was used to obtain net requirements for lactation. The mineral metabolizable coefficient was quantified through observed (treatment means) mineral intake and total fecal and urinary excretions, discounting the estimated endogenous excretions from our proposed models. The nonlinear models of total fecal and urinary mineral excretion were evaluated (observed versus predicted values) using a 5-fold cross validation approach. The models to estimate the sum of endogenous fecal and urinary excretions of P (0.135±0.043 g P/kg BW0.75) and Ca (0.360±0.144 g Ca/kg BW0.75) exhibited suitable precision and accuracy; r = 0.89 and 0.79, concordance correlation coefficient = 0.85 and 0.77, and root mean square prediction error = 24.1 and 20.5% observed means, respectively. Dietary variables (forage level, fiber, starch, crude protein, and ether extract) did not affect the metabolizable coefficient (MC) of P and Ca; therefore, an overall dietary MC of P (0.69±0.01) and Ca (0.65±0.02) were proposed. Our new system estimates lower net and dietary P requirements for lactating dairy cows compared to the NASEM-2021 and NRC-2001 models, but slightly higher Ca requirements than NASEM-2021.This proposed system holds potential to reduce the use of phosphorus in diets for dairy cows, and thus to enhance economic efficiency and environmental sustainability of the dairy industry.

Asunto(s)

Calcio , Lactancia , Fósforo , Animales , Bovinos , Femenino , Fósforo/metabolismo , Fósforo/orina , Calcio/orina , Calcio/metabolismo , Calcio/análisis , Heces/química , Calcio de la Dieta/metabolismo , Calcio de la Dieta/análisis , Alimentación Animal/análisis , Industria Lechera , Leche/metabolismo , Leche/química , Fenómenos Fisiológicos Nutricionales de los Animales , Fósforo Dietético/metabolismo , Fósforo Dietético/orina , Necesidades Nutricionales , Dieta/veterinaria

RESUMEN

This study investigated the effects of ovariectomy and caffeine intake on bone health in rats on calcium-deficient diet. Forty adults female Wistar rats were divided into 4 groups in a 2x2 factorial design: Ovary (OVX/SHAM) and Caffeine (placebo/caffeine). The animals were housed in individual cages for 8 weeks, receiving 18-20g of AIN-93M diet per day, containing 50% of the daily recommended intake of calcium. The rats underwent ovariectomy (OVX) or laparotomy (SHAM) surgery. Caffeine groups received 6mg of caffeine/kg/day. After euthanasia, the tibia and femur were dissected to determine the calcium content and bone fracture strength, respectively. Blood sample was collected to determine serum Ostase. 24-hour urine was analyzed for excreted calcium and NTx. Reduced bone fracture strength and calcium content were observed in OVX and Caffeine groups. When observed separately, OVX group showed increased urinary NTx and lower bone weight, blood ostase, and urinary calcium. Caffeine groups showed elevated urinary calcium. There was a positive correlation between bone fracture strength and calcium content. NTx correlated negatively with bone calcium, fracture strength and thickness. In conclusion, both OVX and caffeine intake debilitate bone health in rats on calcium-deficient diet.

Asunto(s)

Densidad Ósea , Cafeína , Calcio , Ovariectomía , Ratas Wistar , Animales , Femenino , Cafeína/administración & dosificación , Calcio/sangre , Calcio/orina , Calcio/análisis , Densidad Ósea/efectos de los fármacos , Ratas , Calcio de la Dieta/administración & dosificación , Calcio de la Dieta/análisis , Osteoporosis , Fracturas Óseas

RESUMEN

The effect of the substitution of emulsifying salt by the young bamboo flour (BF) (0, 25, 50, 75, 100 % w/w) on requeijão cremoso processed cheese [REQ, REQ 25, REQ 75 REQ 100]) processing was investigated. Gross composition, calcium and sodium values, functional properties (melting rate), color parameters (L, a*, b*, C*, and Whiteness Index, WI), texture profile, fatty acid profile, volatile organic compounds (VOCs), and sensory profiling were evaluated. No effect was observed on the gross composition; however, sodium and melting rate values were decreased, and calcium values presented the opposite behavior. BF could modify the optical parameters, observing an increase in WI values. Higher BF addition increased hardness and lowered elasticity, and regarding the fatty acid profile, there is no significant difference. Different volatile compounds were noted in a proportional form with the BF addition, which was reflected in similar sensory acceptance for REQ 25 and control samples. Although some aspects require further in-depth studies, using BF as a substitute for emulsifying salt in requeijão cremoso processed cheese appears to be a viable option, especially when considering partial replacements.

Asunto(s)

Queso , Harina , Manipulación de Alimentos , Compuestos Orgánicos Volátiles , Queso/análisis , Harina/análisis , Compuestos Orgánicos Volátiles/análisis , Manipulación de Alimentos/métodos , Humanos , Gusto , Ácidos Grasos/análisis , Color , Emulsiones/química , Dureza , Calcio/análisis , Calcio/química

RESUMEN

OBJECTIVE: To assess the effects of arginine, with or without sodium fluoride (NaF; 1,450 ppm), on saliva-derived microcosm biofilms and enamel demineralization. METHODS: Saliva-derived biofilms were grown on bovine enamel blocks in 0.2 % sucrose-containing modified McBain medium, according to six experimental groups: control (McBain 0.2 %); 2.5 % arginine; 8 % arginine; NaF; 2.5 % arginine with NaF; and 8 % arginine with NaF. After 5 days of growth, biofilm viability was assessed by colony-forming units counting, laser scanning confocal microscopy was used to determine biofilm vitality and extracellular polysaccharide (EPS) production, while biofilm metabolism was evaluated using the resazurin assay and lactic acid quantification. Demineralization was evaluated by measuring pH in the culture medium and calcium release. Data were analyzed by Kruskal-Wallis' and Dunn's tests (p < 0.05). RESULTS: 8 % arginine with NaF showed the strongest reduction in total streptococci and total microorganism counts, with no significant difference compared to arginine without NaF. Neither 2.5 % arginine alone nor NaF alone significantly reduced microbial counts compared to the control, although in combination, a reduction in all microbial groups was observed. Similar trends were found for biofilm vitality and EPS, and calcium released to the growth medium. CONCLUSIONS: 8 % Arginine, with or without NaF, exhibited the strongest antimicrobial activity and reduced enamel calcium loss. Also, NaF enhanced the effects of 2.5 % arginine, yielding similar results to 8 % arginine for most parameters analyzed. CLINICAL SIGNIFICANCE: The results provided further evidence on how arginine, with or without NaF, affects oral microcosm biofilms and enamel mineral loss.

Asunto(s)

Arginina , Biopelículas , Cariostáticos , Esmalte Dental , Microscopía Confocal , Saliva , Fluoruro de Sodio , Desmineralización Dental , Biopelículas/efectos de los fármacos , Arginina/farmacología , Fluoruro de Sodio/farmacología , Esmalte Dental/efectos de los fármacos , Esmalte Dental/microbiología , Bovinos , Animales , Desmineralización Dental/prevención & control , Desmineralización Dental/microbiología , Cariostáticos/farmacología , Saliva/microbiología , Saliva/metabolismo , Saliva/efectos de los fármacos , Concentración de Iones de Hidrógeno , Viabilidad Microbiana/efectos de los fármacos , Calcio/análisis , Calcio/metabolismo , Streptococcus/efectos de los fármacos , Xantenos/farmacología , Recuento de Colonia Microbiana , Oxazinas/farmacología

RESUMEN

INTRODUCTION: The decline in dental caries has been attributed to the widespread use of fluoride (F). Two forms of presentation are fluoridated toothpaste (FT) and mouthwash (MW), widely used by the population. MATERIALS AND METHODS: This study aimed to evaluate in vitro the effects of combining FT and MW, whether supplemented with sodium trimetaphosphate (TMP) or not, on dental enamel demineralization. Bovine enamel blocks (n = 60) were selected based on initial surface hardness (SHi) and divided into 5 experimental groups (n = 12 each): I) Placebo Toothpaste (without F/TMP); II) 1100 ppm F Toothpaste (FT); III) 1100F associated with a MW at 100 ppm F (FT + MW 100F); IV) 1100F associated with a MW at 225 ppm F (FT + MW 250F); and V) 1100F associated with a MW at 100 ppm F supplemented with 0.4 % TMP (FT + MW 100F-TMP). The blocks were treated twice a day, undergoing 5 pH cycles over 7 days. Thus, the percentage change in surface hardness (%SH), integrated subsurface hardness loss (ΔKHN), and the concentration of F, phosphorus (P), and calcium (Ca) in the enamel were determined. The data were submitted to ANOVA and Student-Newman-Keuls test (p < 0.001). RESULTS: The 1100F group was statistically inferior to the groups associated with MW for %SH, ΔKHN, and the concentration of P and Ca in the enamel (p < 0.001). Blocks treated with FT + MW 225F and FT + MW 100F-TMP showed significantly lower %SH compared to the other groups (p < 0.001). The FT + MW 100F - TMP group exhibited the lowest depth mineral loss (ΔKHN), and higher concentration de P in enamel (p < 0.001). CONCLUSION: The adjunct use of MW with FT produces a greater protective effect in inhibiting enamel demineralization, and the supplementation of TMP to the MW with 100F provides a superior effect compared to MW with 225F. CLINICAL SIGNIFICANCE: This combination of treatments could be regarded as one of several alternative fluoride supplements for subjects at elevated risk of caries.

Asunto(s)

Cariostáticos , Esmalte Dental , Fluoruros , Dureza , Antisépticos Bucales , Polifosfatos , Desmineralización Dental , Pastas de Dientes , Animales , Bovinos , Polifosfatos/uso terapéutico , Polifosfatos/farmacología , Desmineralización Dental/prevención & control , Esmalte Dental/efectos de los fármacos , Cariostáticos/uso terapéutico , Pastas de Dientes/uso terapéutico , Pastas de Dientes/química , Antisépticos Bucales/uso terapéutico , Fluoruros/uso terapéutico , Concentración de Iones de Hidrógeno , Calcio/uso terapéutico , Calcio/análisis , Ensayo de Materiales

RESUMEN

OBJECTIVE: This scoping review aimed (1) to map, analyse, interpret and synthesize data from in situ studies on which substances were added to acidic beverages to reduce the erosive potential on tooth enamel and (2) to establish the level of evidence of the effectiveness of adding substances to acidic beverages to reduce the erosive potential on tooth enamel. DESIGN: This is a scoping review, according to the methods from the Joanna Briggs Institute and PRISMA-ScR, with high-sensitivity searches in the databases PubMed (MEDLINE), Virtual Health Library, Embase (Elsevier), Scopus (Elsevier), Web of Science and ScienceDirect (Elsevier). In situ studies published in English until December 2022 were included. Studies that did not report a control group were excluded. To map and summarize the results, tables and figures were used. RESULTS: From 895 potentially eligible articles, nine were included. Blackcurrant juices (n = 5) with the addition of calcium (n = 3) or xanthan gum (n = 2) formulations were the most tested in terms of reducing the erosive potential of the beverages on tooth enamel. The profilometer was the equipment of choice (n = 8) for evaluating the loss of enamel structure after the experimental tests. CONCLUSIONS: Calcium and xanthan gum are among the most tested by in situ studies, offering promising results in reducing the erosive potential of fruit juices and carbonated and energy drinks.

Asunto(s)

Bebidas , Esmalte Dental , Erosión de los Dientes , Humanos , Erosión de los Dientes/prevención & control , Erosión de los Dientes/etiología , Esmalte Dental/efectos de los fármacos , Bebidas/efectos adversos , Ácidos/efectos adversos , Concentración de Iones de Hidrógeno , Calcio/análisis , Calcio/uso terapéutico , Jugos de Frutas y Vegetales/efectos adversos

RESUMEN

This research paper analyzes the stability of raw cow milk in the alcohol test and seeks to understand to know the factors that influence milk stability and the occurrence of unstable non-acid milk. Milk samples were collected from the cooling tanks of rural farmers in the state of Paraná twice in summer and twice in winter. The farms were classified according to the production system: pasture with supplementation and feedlot. The following variables were analyzed: stability in the alcohol test, titratable acidity, ionized calcium concentration (iCa), chemical composition of milk, somatic cell count and standard plate count. The results showed that milk stability was greater in winter vs. summer, when the milk contained higher iCa, and in the feedlot vs. pasture system. The Pearson Correlation between variables (ethanol stability, milk composition, iCa, cooling tank temperature, milk volume, number of milking, number of cows milked, fat/protein ratio, distance and travel time) were analyzed. Stability was negatively correlated with iCa concentration and positively with lactose content. Logistic regression of the risk of unstable non-acid milk at 72% alcohol (UNAM72) showed that only iCa and lactose were determinants, while evaluation of the same risk at 78% alcohol revealed iCa, titratable acidity, lactose and milk urea nitrogen as risk factors. Under the dairy farming conditions of Paraná state, the frequency of UNAM72 was low (12.16%) and was higher in summer and in pasture systems with supplementation. In conclusion, in dairy herds bred with high technological level, with adequate nutritional and health management, the frequency of UNAM is low and is related to nutritional management abnd, perhaps, heat stress, factors that alter iCa and lactose levels.

Asunto(s)

Industria Lechera , Leche , Estaciones del Año , Animales , Leche/química , Bovinos , Femenino , Industria Lechera/métodos , Calcio/análisis , Lactosa/análisis , Etanol/análisis , Concentración de Iones de Hidrógeno , Recuento de Células/veterinaria

RESUMEN

OBJECTIVES: To evaluate the anti-demineralizing effect of a mouthwash comprising pomegranate peel extract (PPE 3%), sodium trimetaphosphate (TMP 0.3%), and fluoride (F 225 ppm) in an in situ study, and to assess its irritation potential in an ex vivo study. METHODS: This double-blind crossover study was conducted in four phases with 7 days each. Twelve volunteers used palatal appliances containing enamel blocks, which were subjected to cariogenic challenges. The ETF formulation (PPE + TMP + F, pH 7.0), TF formulation (TMP + F, pH 7.0), deionized water (W, pH 7.0), and essential oil commercial mouthwash (CM, 220 ppm F, pH 4.3) were dropped onto the enamel twice daily. The percentage of surface hardness loss, integrated loss of subsurface hardness, calcium, phosphorus, and fluoride in enamel and biofilms were determined. In addition, alkali-soluble extracellular polysaccharide concentrations were analyzed in the biofilms. The irritation potential was evaluated using the hen's egg chorioallantoic membrane test through the vascular effect produced during 300-s of exposure. RESULTS: ETF was the most efficacious in preventing demineralization. It also showed the highest concentrations of calcium and phosphorus in the enamel and in the biofilm, as well as the lowest amount of extracellular polysaccharides in the biofilm. In the eggs, ETF produced light reddening, whereas CM led to hyperemia and hemorrhage. CONCLUSIONS: The addition of PPE to formulations containing TMP and F increased its anti-demineralizing property, and this formulation presented a lower irritation potential than the CM. CLINICAL RELEVANCE: ETF can be a promising alternative alcohol-free mouthwash in patients at high risk of caries.

Asunto(s)

Antisépticos Bucales , Extractos Vegetales , Granada (Fruta) , Desmineralización Dental , Humanos , Calcio/análisis , Estudios Cruzados , Esmalte Dental , Fluoruros , Dureza , Antisépticos Bucales/química , Antisépticos Bucales/farmacología , Fósforo , Polifosfatos , Desmineralización Dental/prevención & control , Extractos Vegetales/farmacología

RESUMEN

OBJECTIVE: The study aims to evaluate the effect of a glass ionomer cement (GIC; Fuji 9 Gold Label, GC) with added calcium orthophosphate particles and a calcium silicate cement (CSC; Biodentine, Septodont) regarding ion release, degradation in water, mineral content, and mechanical properties of demineralized dentin samples. METHODS: GIC, GIC + 5% DCPD (dicalcium phosphate dihydrate), GIC + 15% DCPD, GIC + 5% ß-TCP (tricalcium phosphate), GIC + 15% ß-TCP (by mass), and CSC were evaluated for Ca2+/Sr2+/F- release in water for 56 days. Cement mass loss was evaluated after 7-day immersion in water. Partially demineralized dentin disks were kept in contact with materials while immersed in simulated body fluid (SBF) at 37 °C for 56 days. The "mineral-to-matrix ratio" (MMR) was determined by ATR-FTIR spectroscopy. Dentin hardness and elastic modulus were obtained by nanoindentation. Samples were observed under scanning and transmission electron microscopy. Data were analyzed by ANOVA/Tukey test (α = 0.05). RESULTS: Ca2+ release from CSC and GIC (µg/cm2) were 4737.0 ± 735.9 and 13.6 ± 1.6, respectively. In relation to the unmodified GIC, the addition of DCPD or ß-TCP increased ion release (p < 0.001). Only the dentin disks in contact with CSC presented higher MMR (p < 0.05) and mechanical properties than those restored with a resin composite used as control (p < 0.05). Mass loss was similar for GIC and CSC; however, the addition of DCPD or ß-TCP increased GIC degradation (p < 0.05). CONCLUSION: Despite the increase in ion release, the additional Ca2+ sources did not impart remineralizing capability to GIC. Both unmodified GIC and CSC showed similar degradation in water. CLINICAL RELEVANCE: CSC was able to promote dentin remineralization.

Asunto(s)

Compuestos de Calcio , Fosfatos de Calcio , Calcio , Cementos de Ionómero Vítreo , Silicatos , Cementos de Ionómero Vítreo/farmacología , Cementos de Ionómero Vítreo/química , Calcio/análisis , Fosfatos/análisis , Cemento de Silicato/análisis , Cemento de Silicato/farmacología , Dentina , Agua/química , Ensayo de Materiales

RESUMEN

OBJECTIVES: To investigate the chemical and mechanical properties of intraradicular dentin submitted to radiotherapy. MATERIALS AND METHODS: Sixteen mandibular incisors were divided into two groups (n = 8): non-irradiated and irradiated. The irradiated teeth were obtained from head and neck radiotherapy patients, with a total dose ranging from 70.2 to 72 Gy divided into 1.8 Gy daily. After sample preparation, intraradicular dentin slices of each root third were evaluated by Raman spectroscopy, energy dispersive spectroscopy and Knoop microhardness test. Data were analyzed by Two-way ANOVA and Tukey's test (α = 0.05). RESULTS: In Raman spectroscopy, carbonate and amide III showed a significant difference for irradiation and third (carbonate p = 0.021 and p < 0.001; amide III p < 0.001 and p = 0.001, respectively). For amide I, there was a significant difference for third (p < 0.001). For carbonate/mineral ratio, there was a significant difference for irradiation (p = 0.0016) and third (p < 0.001), with the irradiated middle third showing the lowest values. For amide I/amide III ratio, there was a significant difference for irradiation (p = 0.005) in the cervical third. In energy dispersive spectroscopy, carbon (p = 0.004; p = 0.020), phosphorus (p < 0.001; p = 0.009) and calcium (p = 0.008; p = 0.007) showed differences for irradiation and third, with the irradiated groups presenting lower values in cervical and middle thirds. For calcium/phosphorus ratio, there was a significant difference for irradiation (p < 0.001) in cervical and middle thirds. Regarding microhardness, there was a significant difference for irradiation (p < 0.001), with all irradiated groups showing lower microhardness values. CONCLUSIONS: The radiotherapy altered the chemical and mechanical properties of intraradicular dentin, mainly in the cervical and middle root thirds.

Asunto(s)

Calcio , Dentina , Humanos , Dentina/química , Calcio/análisis , Incisivo , Carbonatos/análisis , Fósforo/análisis , Amidas/análisis , Ensayo de Materiales

RESUMEN

This study was dedicated to developing analytical methods for determining macronutrients (Ca, K, and Mg) in soy leaf samples with and without petioles. The study's primary purpose was to present Laser-induced breakdown spectroscopy (LIBS) as a viable alternative for directly analyzing leaf samples using chemometric tools to interpret the data obtained. The instrumental condition chosen for LIBS was 70 mJ of laser pulse energy, 1.0 µs of delay time, and 100 µm of spot size, which was applied to 896 samples: 305 of soy without petioles and 591 of soy with petioles. The reference values of the analytes for the proposition of calibration models were obtained using inductively coupled plasma optical emission spectroscopy (ICP-OES) technique. Twelve normalization modes and two calibration strategies were tested to minimize signal variations and sample matrix microheterogeneity. The following were studied: multivariate calibration using partial least squares and univariate calibration using the area and height of several selected emission lines. The notable normalization mode for most models was the Euclidean norm. No analyte showed promising results for univariate calibrations. Micronutrients, P and S, were also tested, and no multivariate models presented satisfactory results. The models obtained for Ca, K, and Mg showed good results. The standard error of calibration ranged from 2.3 g/kg for Ca in soy leaves without petioles with two latent variables to 5.0 g/kg for K in soy leaves with petioles with two latent variables.

Asunto(s)

Rayos Láser , Espectroscopía de Fotoelectrones/métodos , Análisis Espectral/métodos , Calcio/análisis , Calcio/química , Potasio/análisis , Potasio/química , Magnesio/análisis , Magnesio/química

RESUMEN

OBJECTIVE: to evaluate the effect a glass ionomer cement (GIC) containing hydroxyapatite (HAp) or calcium silicate (CaSi) particles on mineral content and mechanical properties of demineralized dentin. Ion release and compressive strength (CS) of the cements were also evaluated. METHODS: GIC (Fuji 9 Gold Label, GC), GIC+ 5%HAp and GIC+ 5%CaSi (by mass) were evaluated. Ion release was determined by induced coupled plasma optical emission spectroscopy (Ca2+/Sr2+) or ion-specific electrode (F-) (n = 3). A composite (Filtek Z250, 3 M ESPE) was used as control in remineralization tests. Demineralized dentin discs were kept in contact with materials in simulated body fluid (SBF) at 37 °C for eight weeks. Mineral:matrix ratio (MMR) was determined by ATR-FTIR spectroscopy (n = 5). Dentin hardness (H) and elastic modulus (E) were determined by nanoindentation (n = 10). CS was tested after 24 h and 7d in deionized water (n = 12). Data were analyzed by ANOVA/Tukey test (α = 0.05). RESULTS: Ca2+ and Sr2+ release was higher for the modified materials (p < 0.05). Only GIC+ 5%HAp showed higher F- release than the control (p < 0.05). All groups showed statistically significant increases in MMR, with no differences among them after 8 weeks (p > 0.05). No differences in dentin H or E were observed among groups (p > 0.05). HAp-modified GIC showed increased initial CS, while adding CaSi had the opposite effect (p < 0.05). After 7 days, GIC+ 5%CaSi presented lower CS in relation to control and GIC+ 5%HAp (p < 0.05). SIGNIFICANCE: GIC modification with HAp or CaSi affected CS and increased ion release; however, none of the groups showed evidence of dentin remineralization in comparison to the negative control.

Asunto(s)

Calcio , Cementos de Ionómero Vítreo , Calcio/análisis , Ensayo de Materiales , Cementos de Ionómero Vítreo/farmacología , Cementos de Ionómero Vítreo/química , Durapatita/farmacología , Durapatita/química , Dentina/química

RESUMEN

This study aimed to evaluate the effect of human saliva in vitro and salivary flow in situ on the roughness and mineral content of bleached enamel. Dental specimens were divided into five groups (n=15): not bleached (NB); bleached (35% hydrogen peroxide) and exposed to distilled water (DW); human saliva in vitro (IV); normal salivary flow in situ (NSF); and low salivary flow (LSF) in situ. Enamel roughness (Ra, Rz) and calcium/phosphorus contents were evaluated with laser profilometry and energy-dispersive spectroscopy, respectively, at baseline (T1), after bleaching (T2), and after seven days (T3). Salivary pH and buffer capacity were evaluated with colorimetric strips and salivary calcium and phosphorus with absorbance spectrophotometry. Data were analyzed with non-parametric tests and linear regression (α=0.05). After contact with saliva, Ra and Rz of LSF=DW>IV=NSF=NB was found. For DW and LSF, the roughness of T1

Asunto(s)

Blanqueadores Dentales , Blanqueamiento de Dientes , Humanos , Blanqueamiento de Dientes/métodos , Blanqueadores Dentales/química , Calcio/análisis , Peróxido de Hidrógeno/química , Esmalte Dental , Minerales/análisis , Minerales/farmacología , Fósforo

RESUMEN

Ca and Mg are the most important chemical elements in lime. Properly measuring Ca and Mg contents is essential to assess the quality of lime products. Quality control guarantees the adequate use of lime in industrial processes, in soils, and helps avoiding adulteration. Proximal sensors can aid in this process by determining Ca and Mg contents easily, rapidly and without producing chemical waste. The objective of this study was to evaluate the use an environmentally-friendly method of analyzing the quality of lime. We studied 1) the use of portable X-ray fluorescence (pXRF) to predict concentrations of Ca and Mg in lime, 2) tested if NixPro™ sensor can improve prediction accuracy and 3) tested if sample preparation methods (grinding) affect analyses. 74 samples of lime were analyzed by two different laboratories (lab. 1 = 38, lab. 2 = 36). All samples submitted to pXRF and NixPro™ analyses. Sensor analyses were done in whole (CP) and ground (AQ) samples to test the effect of sample preparation in prediction performance. High correlation was found between Ca and Mg contents measured via pXRF and laboratory analyses. Mg-CP presented the highest correlation coefficient (r = 0.81); Mg-AQ, the lowest (0.57). Predictions presented good performance (R2 > 0.68); Mg had the best results (0.86). Separating models per laboratory showed that some datasets are harder to model, probably due to variability in the source material (limestone). The addition of NixPro™ data contributed to improve prediction accuracy, although slightly. Predictions using CP samples presented the best results, especially for Mg, indicating that grinding is not necessary. This pioneer study demonstrated that fused proximal sensors can be used to rapidly and easily determine contents of Ca and Mg in soil amendments without producing chemical waste.

Asunto(s)

Calcio , Contaminantes del Suelo , Calcio/análisis , Magnesio/análisis , Contaminantes del Suelo/análisis , Monitoreo del Ambiente/métodos , Espectrometría por Rayos X/métodos , Suelo/química

Asunto(s)

Calcio , AMP Cíclico , Inflamación , Humanos , AMP Cíclico/análisis , Calcio/análisis , Inflamación/diagnóstico

RESUMEN

Developing dental materials for the prevention of remineralization or demineralization is important for high-risk caries patients. This study aimed to evaluate the physicochemical and microbiological effects of adding 45S5 bioglass to resin-modified glass ionomer cement (RMGIC). Samples belonged to the following groups: GIC: conventional glass ionomer cement (Vitro Fil), RMGIC: resin-modified GIC (Vitro Fil LC), and RMGIC/45S5: RMGIC with 10% (wt %) of 45S5. Changes in pH and release of fluoride, calcium, and phosphorus ions under acidic (pH 4) and neutral (pH 7) pH conditions were evaluated. Antibacterial activity was verified based on colony-forming units. Material sorption and solubility were analyzed after bacterial exposure. After 28 days, the bioactivity of the materials was evaluated using scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS). Analysis of variance, post hoc Scheffe, and Tukey (α = 0.05) tests were employed for statistical analysis. RMGIC/45S5 showed higher alkalization activity, calcium release at pH 4 and 7, and sorption than GIC and RMGIC (p < .05). Release of phosphorus and fluoride at pH 4 and 7 was higher for GIC than that for RMGIC and RMGIC/45S5 (p < .05). RMGIC/45S5 showed higher values than RMGIC (p < .05). However, antibacterial activity did not differ among the groups. Precipitates of calcium and phosphorus were visualized in RMGIC/45S5 samples via SEM/EDS. These results indicate that the RMGIC/45S5 promotes alkalization and increases the release of calcium, phosphorus, and fluoride ions, resulting in precipitate deposition rich in calcium and phosphorus, thereby being a promising option to improve the bioactivity of RMGIC.

Asunto(s)

Calcio , Fluoruros , Humanos , Fluoruros/farmacología , Fluoruros/química , Calcio/farmacología , Calcio/análisis , Ensayo de Materiales , Antibacterianos/farmacología , Fósforo/farmacología , Cementos de Ionómero Vítreo/farmacología , Cementos de Ionómero Vítreo/química

RESUMEN

O dejeto de suíno (DLS) é usado como fertilizante cujos nutrientes são perdidos por erosão e contaminam o ambiente se o dejeto é usado inadequadamente. Objetivou-se avaliar o efeito de DLS nosolo e na erosão, num Nitossolo Bruno. Os tratamentos, 0; 50; 100; e 200 m³ ha-1de DLS foram aplicados superficialmente após a semeadura uma vez em cada um dos cinco cultivos, e na palhada do último cultivo, totalizando 0, 250, 500 e 1000 m³ ha-1, na aveia (Avena strigosa), milho (Zea mays), nabo (Raphanus sativus L.) e soja (Glycine max), e nos resíduos de aveia. A chuva simulada (65 mm h-1e 75 minutos) foi aplicada três vezes no milho e quatro vezes no nabo, na soja, e nos resíduos, com um simulador debraços rotativos. Antes e depois da pesquisa, determinou-se o teor de K, Ca e Mg no solo. As chuvas aplicadas em cada momento compuseram um teste (T). O T1 ocorreu após a aplicação do DLS e os demais testes em intervalos que variaram entre 14 e 70 dias, em função do clima. Durante o escoamento, em intervalos de cincominutos coletaram-se amostras de enxurrada para determinar as perdas de água e o teor de K, Ca e Mg na água. O DLS não influenciou o teor dos nutrientes no solo. O teore as perdas totais dos nutrientes foram maiores com 1000 m3ha-1de DLS do que na ausência do dejeto. Com o aumento de escoamento, diminuiu o teor na enxurrada e aumentou a perda total, a qual aumentou também com o aumento de teor dos nutrientes na enxurrada. Recomenda-se aplicar menos do que 500 m3ha-1de DLS total no solo, mesmo em aplicações intervalares, devido à elevada perda de K, Ca e Mg pela erosão hídrica.(AU)

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Pigg slurry (PS) is used as a fertilizer whose nutrients are lost through erosion and contaminate the environment if the manure is used inappropriately. The objective was to evaluate the effect of PS on soil and erosion, in a Alfisol soil. The treatments, 0; 50; 100; and 200 m³ ha-1of PS were applied superficially after sowing once in each of the five crops, and in the straw of the last crop, totaling 0, 250, 500 and 1000 m³ ha-1, in oat (Avena strigosa), corn (Zea mays), turnip (Raphanus sativusL.) and soybean (Glycine max), and in oat residues. The simulated rain (65 mm h-1and 75 minutes) was applied three times to corn and four times to turnip, soybeans, and residues, with a rotating arms simulator. Before and after the research, the content of K, Ca and Mg in thesoil was determined. The rainfall applied at each moment made up a test (T). T1 occurred after the application of the PS and the other tests at intervals that varied between 14 and 70 days, depending on the weather. During the runoff, at five-minute intervals, runoff samples were collected to determine water losses and the content of K, Ca and Mg in the water. The PS did not influence the nutrient content in the soil. The content and total losses of nutrients were higher with 1000 m3ha-1of PS than in theabsence of manure. With the increase in runoff, the content inthe runoff decreased and the total loss increased, which also increased with the increase in the nutrient content in the runoff. It is recommended to apply less than 500 m3ha-1of total PS inthe soil, even in interval applications, due to the high loss of K, Ca and Mg by water erosion.(AU)

Asunto(s)

24444 , Análisis del Suelo , Administración de Residuos/métodos , Potasio/análisis , Porcinos/fisiología , Erosión Hídrica , Calcio/análisis , Magnesio/análisis