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Arginine and sodium fluoride affect the microbial composition and reduce biofilm metabolism and enamel mineral loss in an oral microcosm model.
Sampaio, Caio; Méndez, Daniela Alejandra Cusicanqui; Buzalaf, Marília Afonso Rabelo; Pessan, Juliano Pelim; Cruvinel, Thiago.
Afiliación
  • Sampaio C; Department of Preventive and Restorative Dentistry, São Paulo State University, School of Dentistry, Araçatuba, Brazil.
  • Méndez DAC; Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Vila Universitária SP, Bauru 17012-901, Brazil.
  • Buzalaf MAR; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
  • Pessan JP; Department of Preventive and Restorative Dentistry, São Paulo State University, School of Dentistry, Araçatuba, Brazil.
  • Cruvinel T; Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Vila Universitária SP, Bauru 17012-901, Brazil. Electronic address: thiagocruvinel@fob.usp.br.
J Dent ; 145: 104997, 2024 06.
Article en En | MEDLINE | ID: mdl-38621525
ABSTRACT

OBJECTIVE:

To assess the effects of arginine, with or without sodium fluoride (NaF; 1,450 ppm), on saliva-derived microcosm biofilms and enamel demineralization.

METHODS:

Saliva-derived biofilms were grown on bovine enamel blocks in 0.2 % sucrose-containing modified McBain medium, according to six experimental groups control (McBain 0.2 %); 2.5 % arginine; 8 % arginine; NaF; 2.5 % arginine with NaF; and 8 % arginine with NaF. After 5 days of growth, biofilm viability was assessed by colony-forming units counting, laser scanning confocal microscopy was used to determine biofilm vitality and extracellular polysaccharide (EPS) production, while biofilm metabolism was evaluated using the resazurin assay and lactic acid quantification. Demineralization was evaluated by measuring pH in the culture medium and calcium release. Data were analyzed by Kruskal-Wallis' and Dunn's tests (p < 0.05).

RESULTS:

8 % arginine with NaF showed the strongest reduction in total streptococci and total microorganism counts, with no significant difference compared to arginine without NaF. Neither 2.5 % arginine alone nor NaF alone significantly reduced microbial counts compared to the control, although in combination, a reduction in all microbial groups was observed. Similar trends were found for biofilm vitality and EPS, and calcium released to the growth medium.

CONCLUSIONS:

8 % Arginine, with or without NaF, exhibited the strongest antimicrobial activity and reduced enamel calcium loss. Also, NaF enhanced the effects of 2.5 % arginine, yielding similar results to 8 % arginine for most parameters analyzed. CLINICAL

SIGNIFICANCE:

The results provided further evidence on how arginine, with or without NaF, affects oral microcosm biofilms and enamel mineral loss.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arginina / Saliva / Fluoruro de Sodio / Cariostáticos / Desmineralización Dental / Microscopía Confocal / Biopelículas / Esmalte Dental Límite: Animals Idioma: En Revista: J Dent Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arginina / Saliva / Fluoruro de Sodio / Cariostáticos / Desmineralización Dental / Microscopía Confocal / Biopelículas / Esmalte Dental Límite: Animals Idioma: En Revista: J Dent Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido