RESUMEN
JC polyomavirus (JCPyV) is the causative agent for progressive multifocal leukoencephalopathy (PML) in immunocompromised patients. More than 40% of healthy population excretes JCPyV particles in their urine. As JCPyV is ubiquitous in human, the definition of genotype distribution can help trace population migration. In this study, to define the frequency of JCPyV in southwest of Iran, urine samples of 161 volunteers including 80 healthy individuals and 81 HIV-infected patients were collected. PCR assays and sequence analysis were performed using JCPyV-specific primers designed against VP1 coding region. JCPyV DNA was detected in 65 out of 81 urine samples (80.2%) of HIV-infected, and in 43 out of 80 urine samples (53.8%) of healthy individuals (P = 0.001). The shedding of JCPyV among HIV-infected patients revealed an age-related pattern while such relationship was not observed in healthy individuals group. The most common genotype found in this region was genotype 3A (80.8%), followed by genotype 2D (11.5%), 4 (3.8%), and 7 (3.8%). The frequency of JCPyV in the urine of HIV-infected patients was found significantly higher than in the healthy individuals (P = 0.001).
Asunto(s)
Infecciones por VIH/complicaciones , Virus JC/aislamiento & purificación , Infecciones por Polyomavirus/epidemiología , Infecciones Tumorales por Virus/epidemiología , Adolescente , Adulto , Factores de Edad , Proteínas de la Cápside/genética , Niño , Preescolar , ADN Viral/orina , Femenino , Genotipo , Infecciones por VIH/epidemiología , Infecciones por VIH/virología , Voluntarios Sanos , Humanos , Huésped Inmunocomprometido , Lactante , Recién Nacido , Irán/epidemiología , Virus JC/genética , Masculino , Persona de Mediana Edad , Infecciones por Polyomavirus/orina , Esparcimiento de Virus , Adulto JovenRESUMEN
Canine kobuvirus (CaKV) is a member of the Picornaviridae family and the Kobuvirus genus. CaKV was first described in fecal samples from diarrheic dogs in the USA in 2011, with subsequent reports in the UK, Italy, South Korea, China, Tanzania, and Japan. CaKV is frequently identified in feces of animals with or without clinical signs of gastroenteritis. The present study investigated the presence of CaKV in fecal samples from 53 diarrheic dogs from Londrina, southern Brazil. Using a RT-PCR assay, CaKV RNA was identified in three dogs, resulting in an overall occurrence rate of 5.7%. In addition, coinfection with canine parvovirus subtype 2b was detected in all CaKV-positive diarrheic fecal samples. Using a phylogenetic analysis based on the VP1 gene sequence, the Brazilian CaKV field strains were found to be very similar to a previously identified CaKV strain from Brazil that was found in the tissue of a puppy and were also found to be clustered with other CaKV strains detected worldwide and other kobuvirus strains identified in mouse, feline, and human hosts.
Asunto(s)
Diarrea/veterinaria , Enfermedades de los Perros/virología , Heces/virología , Kobuvirus/aislamiento & purificación , Infecciones por Parvoviridae/veterinaria , Parvovirus/aislamiento & purificación , Animales , Brasil , Coinfección/veterinaria , Coinfección/virología , Diarrea/virología , Perros , Kobuvirus/clasificación , Kobuvirus/genética , Infecciones por Parvoviridae/virología , Parvovirus/clasificación , Parvovirus/genética , Filogenia , ARN Viral/genéticaRESUMEN
Background: Foot and mouth disease (FMD) is the causative agent and one of the most transmissible livestock diseaseswhich cause important economic losses. The genome of FMD virus is a positive-sense, single-stranded RNA and it wrappedwith 60 copies of 4 structural proteins, VP1, VP2, VP3 and VP4. The VP1 is a major immunogenic antigen with criticalepitopes for inducing immune responses. The current vaccine, which successfully prevents disease, includes inactivatedwhole virus antigen. However, it is not without problems. The aim of this study is enhancement of immune responsesagainst Iranian isolate of FMD-type O/IRN/1/2010 based on VP1 and human HSP70 fusion protein in BALB/c mice.Materials, Methods & Results: In this study FMD virus type O/IRN/2010 was isolated from vesicles of the infected cattlein Qoum, and serotyped as a new linage of type O (PanAsia-linage 2) in Iran. The isolated FMD virus was propagated onIBRS2 cell line and whole RNA of the infected cells was extracted by commercial kit instruction. The extracted RNA wasamplifi ed using VP1 gene-specifi c primer pairs by means of one-step RT-PCR. The specifi c primer pair was designed byAllelID6 software. There are sequences of Kpn I and BamH I restriction enzymes and three overhanging nucleotides atthe start of forward and reverse primers, respectively. The VP1 nucleotide sequence was deposit in Genbank-NCBI database under accession number JN 676146. The purifi ed VP1 gene was sub-cloned into PTZ57R/T vector. Then digestedVP1 gene by KpnI and BamHI enzymes was ligated in pcDNA3.1+ vector as a DNA vaccine. Also, the improved DNAimmunization system was constructed using pcDNA3.1+ plasmid contains VP1 gene of Iranian isolate FMD virus typeO/IRN/1/2010 and human HSP70 gene and expression of VP1-HSP70 fusion protein confi rmed in BHKT7 cell line byGuinea pig specifi c polyclonal antibody against FMD virus type O and conjugated rabbit...
Asunto(s)
Animales , Ratones , Genoma Viral , Proteínas Virales/aislamiento & purificación , Vacunas de ADN/análisis , Virus de la Fiebre Aftosa/inmunología , Virus de la Fiebre Aftosa/aislamiento & purificación , Ratones Endogámicos BALB CRESUMEN
Background: Foot and mouth disease (FMD) is the causative agent and one of the most transmissible livestock diseaseswhich cause important economic losses. The genome of FMD virus is a positive-sense, single-stranded RNA and it wrappedwith 60 copies of 4 structural proteins, VP1, VP2, VP3 and VP4. The VP1 is a major immunogenic antigen with criticalepitopes for inducing immune responses. The current vaccine, which successfully prevents disease, includes inactivatedwhole virus antigen. However, it is not without problems. The aim of this study is enhancement of immune responsesagainst Iranian isolate of FMD-type O/IRN/1/2010 based on VP1 and human HSP70 fusion protein in BALB/c mice.Materials, Methods & Results: In this study FMD virus type O/IRN/2010 was isolated from vesicles of the infected cattlein Qoum, and serotyped as a new linage of type O (PanAsia-linage 2) in Iran. The isolated FMD virus was propagated onIBRS2 cell line and whole RNA of the infected cells was extracted by commercial kit instruction. The extracted RNA wasamplifi ed using VP1 gene-specifi c primer pairs by means of one-step RT-PCR. The specifi c primer pair was designed byAllelID6 software. There are sequences of Kpn I and BamH I restriction enzymes and three overhanging nucleotides atthe start of forward and reverse primers, respectively. The VP1 nucleotide sequence was deposit in Genbank-NCBI database under accession number JN 676146. The purifi ed VP1 gene was sub-cloned into PTZ57R/T vector. Then digestedVP1 gene by KpnI and BamHI enzymes was ligated in pcDNA3.1+ vector as a DNA vaccine. Also, the improved DNAimmunization system was constructed using pcDNA3.1+ plasmid contains VP1 gene of Iranian isolate FMD virus typeO/IRN/1/2010 and human HSP70 gene and expression of VP1-HSP70 fusion protein confi rmed in BHKT7 cell line byGuinea pig specifi c polyclonal antibody against FMD virus type O and conjugated rabbit...(AU)
Asunto(s)
Animales , Ratones , Genoma Viral , Proteínas Virales/aislamiento & purificación , Virus de la Fiebre Aftosa/inmunología , Virus de la Fiebre Aftosa/aislamiento & purificación , Vacunas de ADN/análisis , Ratones Endogámicos BALB C , Proteínas HSP70 de Choque TérmicoRESUMEN
In the present study, epidemiological survey and molecular characterization of hepatitis A virus during an outbreak in five Tunisian childcare centers in El-Mahres during October and November 2006 were carried out. Five well-water and five drinking water samples were included in the present study. Serological investigation and molecular characterization were carried out. All patients were IgM seropositive and the viral genome was detected in all clinical and well-water samples whereas it was not detected in drinking water from the five childcare centers. Sequence analysis showed that all Tunisian strains belong to sub-genotype IA. The genetic profile of the VP1/2A junction showed that the outbreak isolates underwent an amino acid substitution which was absent in virus's strains detected previously in Tunisia. Further studies need to be conducted to evaluate the emergence of the virus's strains in clinical and water samples and more epidemiological data need to be collected about the risk factors which may contribute to acute hepatitis.
Asunto(s)
Humanos , Brotes de Enfermedades , Inmunoglobulina M , Análisis de Secuencia de ADN , Análisis de Secuencia de Proteína , Virus de Hepatitis/genética , Técnicas y Procedimientos Diagnósticos , Métodos , Pacientes , Muestras de AguaRESUMEN
In the present study, epidemiological survey and molecular characterization of hepatitis A virus during an outbreak in five Tunisian childcare centers in El-Mahres during October and November 2006 were carried out. Five well-water and five drinking water samples were included in the present study. Serological investigation and molecular characterization were carried out. All patients were IgM seropositive and the viral genome was detected in all clinical and well-water samples whereas it was not detected in drinking water from the five childcare centers. Sequence analysis showed that all Tunisian strains belong to sub-genotype IA. The genetic profile of the VP1/2A junction showed that the outbreak isolates underwent an amino acid substitution which was absent in virus's strains detected previously in Tunisia. Further studies need to be conducted to evaluate the emergence of the virus's strains in clinical and water samples and more epidemiological data need to be collected about the risk factors which may contribute to acute hepatitis.
RESUMEN
In the present study, epidemiological survey and molecular characterization of hepatitis A virus during an outbreak in five Tunisian childcare centers in El-Mahres during October and November 2006 were carried out. Five well-water and five drinking water samples were included in the present study. Serological investigation and molecular characterization were carried out. All patients were IgM seropositive and the viral genome was detected in all clinical and well-water samples whereas it was not detected in drinking water from the five childcare centers. Sequence analysis showed that all Tunisian strains belong to sub-genotype IA. The genetic profile of the VP1/2A junction showed that the outbreak isolates underwent an amino acid substitution which was absent in virus's strains detected previously in Tunisia. Further studies need to be conducted to evaluate the emergence of the virus's strains in clinical and water samples and more epidemiological data need to be collected about the risk factors which may contribute to acute hepatitis.