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Detection of canine kobuvirus RNA in diarrheic fecal samples of dogs with parvoviruses.
Miyabe, Flavia Megumi; Ribeiro, Juliane; Alfieri, Alice Fernandes; Alfieri, Amauri Alcindo.
Afiliación
  • Miyabe FM; Laboratory of Animal Virology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR, 86057-970, Brazil.
  • Ribeiro J; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR, 86057-970, Brazil.
  • Alfieri AF; Laboratory of Animal Virology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR, 86057-970, Brazil.
  • Alfieri AA; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR, 86057-970, Brazil.
Braz J Microbiol ; 50(3): 871-874, 2019 Jul.
Article en En | MEDLINE | ID: mdl-31140097
Canine kobuvirus (CaKV) is a member of the Picornaviridae family and the Kobuvirus genus. CaKV was first described in fecal samples from diarrheic dogs in the USA in 2011, with subsequent reports in the UK, Italy, South Korea, China, Tanzania, and Japan. CaKV is frequently identified in feces of animals with or without clinical signs of gastroenteritis. The present study investigated the presence of CaKV in fecal samples from 53 diarrheic dogs from Londrina, southern Brazil. Using a RT-PCR assay, CaKV RNA was identified in three dogs, resulting in an overall occurrence rate of 5.7%. In addition, coinfection with canine parvovirus subtype 2b was detected in all CaKV-positive diarrheic fecal samples. Using a phylogenetic analysis based on the VP1 gene sequence, the Brazilian CaKV field strains were found to be very similar to a previously identified CaKV strain from Brazil that was found in the tissue of a puppy and were also found to be clustered with other CaKV strains detected worldwide and other kobuvirus strains identified in mouse, feline, and human hosts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parvovirus / Infecciones por Parvoviridae / Diarrea / Enfermedades de los Perros / Kobuvirus / Heces Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parvovirus / Infecciones por Parvoviridae / Diarrea / Enfermedades de los Perros / Kobuvirus / Heces Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil